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Morphological and Molecular Characterization of Diplogasteroides sp., a Cryptic Population of the Haslacheri Group (Diplogastridae), and Parasitorhabditis terebranus (Rhabditidae) from Korea

Abraham Okki Mwamula
Abraham Okki Mwamula
, 
Sang Myeong Lee
Sang Myeong Lee
, 
Young Hak Jung
Young Hak Jung
, 
Ho-wook Lee
Ho-wook Lee
, 
Yi Seul Kim
Yi Seul Kim
, 
Young Ho Kim
Young Ho Kim
 oraz 
Dong Woon Lee
Dong Woon Lee
   | 23 maj 2023
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Article Category: Research Paper
Data publikacji: 23 maj 2023
Zakres stron: -
Otrzymano: 29 sty 2023
DOI: https://doi.org/10.2478/jofnem-2023-0017
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© 2023 Abraham Okki Mwamula et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1:

Photomicrographs of Diplogasteroides sp. (A-C, F, and G) and Parasitorhabditis terebranus (D, E, and H-J). Diplogasteroides sp. A, B: Variation in female anterior body region; C: Female tail; F and G: Variation in male tail and copulatory apparatus shape. Parasitorhabditis terebranus D, E, and H: Variation in female anterior body region; I: Male tail with copulatory apparatus; J: Female tail; (Scale bars: A-J = 10 μm).
Photomicrographs of Diplogasteroides sp. (A-C, F, and G) and Parasitorhabditis terebranus (D, E, and H-J). Diplogasteroides sp. A, B: Variation in female anterior body region; C: Female tail; F and G: Variation in male tail and copulatory apparatus shape. Parasitorhabditis terebranus D, E, and H: Variation in female anterior body region; I: Male tail with copulatory apparatus; J: Female tail; (Scale bars: A-J = 10 μm).

Figure 2:

Illustrations of Diplogasteroides sp. (A-H): A: Female whole body; B: Male whole body; C: Male tail region including copulatory apparatus and the arrangement of genital papillae; D-F: Variation in shape of gubernaculum; G: Female tail region; H: Female anterior region, including the shape of pharynx. Abbreviations: Genital papillae arrangement from ventral side (v + number); lateral/dorsal sides (v3d; ad; pd); phasmid (ph); and deirid (d).
Illustrations of Diplogasteroides sp. (A-H): A: Female whole body; B: Male whole body; C: Male tail region including copulatory apparatus and the arrangement of genital papillae; D-F: Variation in shape of gubernaculum; G: Female tail region; H: Female anterior region, including the shape of pharynx. Abbreviations: Genital papillae arrangement from ventral side (v + number); lateral/dorsal sides (v3d; ad; pd); phasmid (ph); and deirid (d).

Figure 3:

Illustrations of Parasitorhabditis terebranus (A-G): A: Male whole body; B: Female whole body; C: Female anterior region; D and F: Female tail region including variation in vulval lip shape; E: Male tail in right lateral view, with arrangement of bursal rays; G: Male tail in ventral view, with arrangement of bursal rays.
Illustrations of Parasitorhabditis terebranus (A-G): A: Male whole body; B: Female whole body; C: Female anterior region; D and F: Female tail region including variation in vulval lip shape; E: Male tail in right lateral view, with arrangement of bursal rays; G: Male tail in ventral view, with arrangement of bursal rays.

Figure 4:

Bayesian tree inferred under the GTR + I + G model from I8S-rRNA sequences of Diplogasteroides spp., Parasitorhabditis spp., and other closely related species from other genera. Posterior probability values exceeding 50% are given on appropriate clades. The studied population is indicated in bold. Outgroup taxon: Rhabditoides inermiformis and Leptolaimus donsi.
Bayesian tree inferred under the GTR + I + G model from I8S-rRNA sequences of Diplogasteroides spp., Parasitorhabditis spp., and other closely related species from other genera. Posterior probability values exceeding 50% are given on appropriate clades. The studied population is indicated in bold. Outgroup taxon: Rhabditoides inermiformis and Leptolaimus donsi.

Figure 5:

Bayesian tree inferred under the GTR + I + G model from D2-D3 expansion segment of 28S-rRNA partial sequences of Diplogasteroides spp., Parasitorhabditis spp., and other closely related species from other genera. Posterior probability values exceeding 50% are given on appropriate clades. The studied population is indicated in bold. Outgroup taxon: Rhabditoides regina.
Bayesian tree inferred under the GTR + I + G model from D2-D3 expansion segment of 28S-rRNA partial sequences of Diplogasteroides spp., Parasitorhabditis spp., and other closely related species from other genera. Posterior probability values exceeding 50% are given on appropriate clades. The studied population is indicated in bold. Outgroup taxon: Rhabditoides regina.

Figure 6:

Bayesian tree inferred under the GTR + I + G model from COI partial sequences of Diplogasteroides spp., Parasitorhabditis spp., and members of closely related genera. Posterior probability values exceeding 50% are given on appropriate clades. The studied population is indicated in bold. Outgroup taxon: Ascaridia columbae.
Bayesian tree inferred under the GTR + I + G model from COI partial sequences of Diplogasteroides spp., Parasitorhabditis spp., and members of closely related genera. Posterior probability values exceeding 50% are given on appropriate clades. The studied population is indicated in bold. Outgroup taxon: Ascaridia columbae.

Comparison of morphometries of Diplogasteroides sp. and Parasitorhabditis terebranus from Korea with topotype populations.a

Character Diplogasteroides sp. D. haslacheri Parasitorhabditis terebranus
Korea (Current study) Rühm (1956), and Kanzaki et al. (2002) Korea (Current study) USA (Massey, 1974)
♀♀ ♂♂ ♀♀b ♂♂ ♀♀ ♂♂ ♀♀ ♂♂
N 20 20 ? ? 20 20 ? ?
L 1061,3±106.9(855.3-1258.3) 909.O±82.8(777.6-1068.1) 825-1005 750-825 109δ.4±78.6(922.4-1244.4) 1015.8±96.9(841.0-1195.6) 770-810 750
A 29.4±2.1(25.1-32.3) 29.2±0.8(27.7-30.5) 26.1 26.2-26.8 26.4±2.9(20.5-30.0) 26.0±2.2(21.1-29.4) 19.7-20.1 19.5
B 6.2±0.5(5.4-7.5) 5.8±0.4(5.2-6.3) 5.6-6.1 5.1-5.2 5.5±0.4(4.6-6.1) 5.2±0.4(4.6-6.1) 4.2-4.3 4.1
C 11.7±0.8(9.8-13.1) I4.9±0.9(13.2-16.2) 9.4-9.6 12.4-15.3 27.1±2.2(22.8-30.2) 27.4±2.3(22.1-30.5) 26.2-27.3 27.3
c’ 4.2±0.3(3.5-4.7) 2.5±0.2(2.2-2.9) 5.0 2.4 1.9±0.2(1.6-2.1) 1.7±0.1(1.4-1.9) - -
V 51.6±1.1(50.3-53.2) - 50.3-52.3 - 93.3±0.5(91.9-93.9) - 93 -
Lip height - - - - 3.3±0.1(3.0-3.6) 3.1 ±0.2(2.8-3.5) - -
Lip diameter 11.9±0.5(10.6-12.6) 11.9±0.6(11.0-12.6) - - 12.6±0.7(11.6-14.0) 12.1 ±0.7(11.0-13.5) - -
Stoma 11.1±0.8(9.8-12.3) 11.0±0.7(9.0-11.8) - - 20.9±1.4(18.5-23.0) 20.3±1.1(18.0-22.0) 21 -
Anterior to median bulb valve 98.2±6.3(87.6-112.7) 90.4±5.3(82.4-102.2) - - 107.2±7.4(95.8-120.2) 101,4±5.1(93.3-112.9) - -
Excretory pore 1340±8.3(123.0-155.4) 126.8±5.7(114.8-138.0) - - 1540±8.7(135.0-162.7) 153.6±8.6(136.0-164.0) - -
Pharynx length 171.4±11.9(154.1-194.2) 156.3±8.6(145.0-170.0) - - 201.0±9.3 (191.3-223.0) 194.3±8.1(181.3-208.5) - -
Maximum body diameter 36.2±3.5(29.0-42.1) 31.7±3.0(27.4-37.7) - - 41.9±4.7(36.0-51.5) 39.1±2.7(33.3-43.0) - -
Vulval body diameter 38.1±3.6(33.6-43.6) - - - 35.2±2.9(30.8-42.6) - - -
Vulva to anus length - - - - 32.5±2.6(28.0-38.0) - - -
Vulva to tail tip - - - - 73.1±3.5(64.0-77.9) - - -
Anal / cloacal body diameter 21.5±1.4(19.2-24.8) 24.2±1.6(22.0-27.0) - - 21.4±1.5(18.9-24.5) 21.8±1.6(19.8-24.9) - -
Tail length 90.5±9.5(73.0-104.6) 60.8±4.6(50.0-69.5) - - 40.6±3.0(36.0-45.8) 37.0±2.5(31.5-41.0) - -
Spicules - 35.5±2.1(30.0-38.0) - 27-30 - 36.6±1.7(33.0-38.6) - 34
Gubernaculum - 17.9±1.5(15.7-21.0) - 15-17 - 16.5±0.6(15.2-18.0) - 17
Tail spike length - 19.1 ±2.3(16.0-23.4) - - - - - -
eISSN:
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Język:
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Częstotliwość wydawania:
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Dziedziny czasopisma:
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