<abstract xmlns="http://www.w3.org/1999/xhtml">

<bold>Objectives.</bold> The present study was designed to assess whether apoptosis-related genes as <italic>parp-1</italic> and <italic>bax</italic> could be targets for treatment of diabetes mellitus and whether vitamin D may exert beneficial effects.
<bold>Methods.</bold> Vitamin D3 treatment for 4 weeks, starting after 4 weeks of the diabetes duration. The expression of <italic>parp-1</italic> and <italic>bax</italic> genes was estimated on mRNA levels using real time quantitative polymerase chain reaction.
<bold>Results.</bold> After 8 weeks, diabetic rats had weight loss, while blood glucose was increased about 4.9-fold compared to control group. Vitamin D3 administration to diabetic animals had no effect on these parameters. It was found that total serum alkaline phosphatase activity was significantly elevated in diabetic rats as compared to control animals and was restored by vitamin D3. Diabetes was accompanied by reduction of nicotinamidadenindinucleotide, a substrate of poly-ADP-ribosylation, level by 31.7% as compared to control rats, which was not reversed in response to vitamin D3 treatment. In diabetic hearts, the mRNA expression level of <italic>parp-1</italic> gene was 2.8-fold higher compared to control rats and partially decreased by vitamin D3 treatment. Less significant alterations were observed in diabetic hearts for the mRNA expression level of <italic>bax</italic> gene that was 2.0-fold higher compared to control animals and vitamin D3 normalized it. These results indicate that cardiomyocytes have a tendency to apoptosis.
<bold>Conclusions.</bold> The findings suggest that investigated genes can be targets at the transcriptional level for vitamin D action that may be contributed to the improving metabolic/signaling pathways induced by diabetes mellitus.
</abstract>

Objectives. The present study was designed to assess whether apoptosis-related genes as parp-1 and bax could be targets for treatment of diabetes mellitus and whether vitamin D may exert beneficial effects.
Methods. Vitamin D3 treatment for 4 weeks, starting after 4 weeks of the diabetes duration. The expression of parp-1 and bax genes was estimated on mRNA levels using real time quantitative polymerase chain reaction.
Results. After 8 weeks, diabetic rats had weight loss, while blood glucose was increased about 4.9-fold compared to control group. Vitamin D3 administration to diabetic animals had no effect on these parameters. It was found that total serum alkaline phosphatase activity was significantly elevated in diabetic rats as compared to control animals and was restored by vitamin D3. Diabetes was accompanied by reduction of nicotinamidadenindinucleotide, a substrate of poly-ADP-ribosylation, level by 31.7% as compared to control rats, which was not reversed in response to vitamin D3 treatment. In diabetic hearts, the mRNA expression level of parp-1 gene was 2.8-fold higher compared to control rats and partially decreased by vitamin D3 treatment. Less significant alterations were observed in diabetic hearts for the mRNA expression level of bax gene that was 2.0-fold higher compared to control animals and vitamin D3 normalized it. These results indicate that cardiomyocytes have a tendency to apoptosis.
Conclusions. The findings suggest that investigated genes can be targets at the transcriptional level for vitamin D action that may be contributed to the improving metabolic/signaling pathways induced by diabetes mellitus.

The <italic>parp-1</italic> and <italic>bax</italic> genes as potential targets for treatment of the heart functioning impairments induced by type 1 diabetes mellitus

Department of Vitamin and Coenzyme Biochemistry

Horbachevsky Ternopil State Medical University

Endocrine Regulations

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The  and  genes as potential targets for treatment of the heart functioning impairments induced by type 1 diabetes mellitus

Objectives. The present study was designed to assess whether apoptosis-related genes as parp-1 and bax could be targets for treatment of diabetes mellitus and...

The parp-1 and bax genes as potential targets for treatment of the heart functioning impairments induced by type 1 diabetes mellitus

Data publikacji: 21 maj 2021

Zakres stron: 61 - 71

DOI: https://doi.org/10.2478/enr-2021-0008

Słowa kluczowe
type 1 diabetes mellitus, mRNA expression, gene, gene, vitamin D

© 2021 Tamara Kuchmerovska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The parp-1 and bax genes as potential targets for treatment of the heart functioning impairments induced by type 1 diabetes mellitus

Data publikacji: 21 maj 2021

Zakres stron: 61 - 71

DOI: https://doi.org/10.2478/enr-2021-0008

Słowa kluczowetype 1 diabetes mellitus, mRNA expression, gene, gene, vitamin D

© 2021 Tamara Kuchmerovska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Słowa kluczowe
type 1 diabetes mellitus, mRNA expression, gene, gene, vitamin D