Established and advanced approaches for recovery of microbial polyhydroxyalkanoate (PHA) biopolyesters from surrounding microbial biomass
| 19 lip 2020
O artykule
Article Category: Review
Data publikacji: 19 lip 2020
Zakres stron: 113 - 126
DOI: https://doi.org/10.2478/ebtj-2020-0013
Słowa kluczowe
© 2020 Martin Koller, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.
Figure 1
Comparison of different PHA isolation methods (update and extensive expansion of [14])
| Recovery method | Method suitable for strains / types of PHA: | Time expenditure | Throughput of chemicals | Scalability to industrial scale? | Recovery yields | Product purity | Reduction of molar mass | Selected references |
|---|---|---|---|---|---|---|---|---|
| Chloroform extraction | All strains | Medium | High | No | High | High | Medium | [ |
| Combined application of chloroform and aqueous NaOCl solution | All strains | Medium | High | No | High | High | Medium | [ |
| “Agroferm method” based on cyclic carbonates) | All strains | Medium | Medium | Yes | Medium | High | High | [ |
| Brazilian “PHB/ISA method” using fusel alcohols | All strains | High | Extraction solvents available in-house | Yes | High | Low/ Medium | High | [ |
| Extraction with lactic acid esters | All strains | Medium/ High | High | Yes | Low/ Medium | Medium | High | [ |
| Ethanol extraction | Mutant strains, | Medium | Low- Medium (easily recyclable solvent) | Yes | Low | Low/ Medium | High | [ |
| GBL extraction | All strains | Medium | High | No | Low | Medium | Low | [ |
| Cyclohexanone extraction | All strains | Medium | High | Yes | Medium | Medium | Low | [ |
| Dimethyl carbonate (DMC) | All strains | Low | Medium | To be tested | Medium | High | Medium | [ |
| 1,2-propylene carbonate | All strains | Low | Low- Medium (easily recyclable solvent) | Yes | Medium | High | Medium | |
| Acetone under reflux | Pseudomonads ( | Medium | Medium (easily recyclable solvent) | No | High | High | Low | [ |
| Acetone under elevated temperature and pressure | All strains | Low | Medium (easily recyclable solvent) | Adequate equipment to be developed; data only for lab-scale | High | High | Low | [ |
| Ethyl acetate | All strains | Medium | Medium (easily recyclable solvent) | To be tested | High | Medium | Medium-High | [ |
| Supercritical sCO2 | Tested only for restricted number of strains; | Medium | High (addition of modifiers needed) | No | Low | Low | Low | [ |
| C2mim][MeO(H)PO2] | All strains | Low | High | To be tested | Medium | Medium | Low | [ |
| Digestion of non- PHA cell material by enzyme cocktails (“ICI process”) | All strains | Low | High | Yes | High | Low | No | [ |
| Biological digestion of non-PHA biomass by | All non-toxic microbes | High | No | Doubtful | High | Medium | Low | [ |
| Alkaline (NaOH) digestion of non-PHA cell material | Rec. | Low | Low | Yes | Medium | High | Depends on incubation time and temperature | [ |
| Combined SDS/ hypochlorite treatment | All strains | Medium | Medium | Yes | High | High | Negligible (depends on hypochlorite concentration) | [ |
| Hypochlorite digestion of non-PHA cell material | All strains | Medium | Medium | No | Medium | Medium | Medium-High | [ |
| Switchable anionic surfactants (NH-4laurate) | All strains | Medium | Medium | Yes | Medium | High | Low | [ |
| Mechanical disintegration of PHA-rich cells (High-pressure homogenization, ultrasonication, vortexing, bead mills) | All strains (Ultrasonication for fragile strains like | Low | No | Yes | Medium | Low | No | [ |
| Cell disruption in hypotonic medium | Tested for highly osmophilic strains like | Low | No | Yes | High | Medium | No | [ |