<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Silver nanoparticles (AgNPs) are nanomaterials obtained by nanotechnology and due to their antimicrobial properties have a major importance in the control of various species of bacteria, fungi and viruses, with applications in medicine, cosmetics or food industry. The goal of the paper was to present the results of the research carried out on rapid extracellular biosynthesis of silver nanoparticles mediated by culture filtrate of lactic acid bacteria <italic>Lactobacillus</italic> sp. strain LCM5 and to assess the antimicrobial activity. Analysis of transmission electron microscopy (TEM) micrographs evidenced that the size of AgNPs synthesized using culture filtrates of lactic acid bacteria strain LCM5 ranged between 3 and 35 nm diameter, with an average particle size of 13.84±4.56 nm. AgNPs presented a good dispersion, approximately spherical shape, with parallel stripes certifying crystal structure. Frequency distribution revealed that preponderant dimensions of biosynthesized AgNPs were below 20 nm (94%). Antimicrobial activity of AgNPs was variable depending on both species and group of test microorganisms (bacteria or fungi) involved. Diameter of growth inhibition zone of <italic>Aspergillus flavus</italic> and <italic>Aspergillus ochraceus</italic> caused by silver nanoparticles synthesized by lactic acid bacteria strain LCM5 were similar (12.39 ± 0.61mm and 12.86 ± 0.78 mm) but significant stronger inhibition was registered against <italic>Penicillium expansum</italic> (15.87 ± 1.01mm). The effectiveness of biosynthesized silver nanoparticles was more pronounced against Gram-negative bacteria <italic>Chromobacterium violaceum</italic> with larger zone of inhibition (18 ± 0.69 mm diameter) when compared to those from fungi. Results recommend the silver nanoparticles biosynthesized using culture filtrate of the lactic acid bacteria <italic>Lactobacillus</italic> sp. strain LCM5 for biotechnological purposes, as promising antimicrobial agents.</p></abstract>

Silver nanoparticles (AgNPs) are nanomaterials obtained by nanotechnology and due to their antimicrobial properties have a major importance in the control of various species of bacteria, fungi and viruses, with applications in medicine, cosmetics or food industry. The goal of the paper was to present the results of the research carried out on rapid extracellular biosynthesis of silver nanoparticles mediated by culture filtrate of lactic acid bacteria Lactobacillus sp. strain LCM5 and to assess the antimicrobial activity. Analysis of transmission electron microscopy (TEM) micrographs evidenced that the size of AgNPs synthesized using culture filtrates of lactic acid bacteria strain LCM5 ranged between 3 and 35 nm diameter, with an average particle size of 13.84±4.56 nm. AgNPs presented a good dispersion, approximately spherical shape, with parallel stripes certifying crystal structure. Frequency distribution revealed that preponderant dimensions of biosynthesized AgNPs were below 20 nm (94%). Antimicrobial activity of AgNPs was variable depending on both species and group of test microorganisms (bacteria or fungi) involved. Diameter of growth inhibition zone of Aspergillus flavus and Aspergillus ochraceus caused by silver nanoparticles synthesized by lactic acid bacteria strain LCM5 were similar (12.39 ± 0.61mm and 12.86 ± 0.78 mm) but significant stronger inhibition was registered against Penicillium expansum (15.87 ± 1.01mm). The effectiveness of biosynthesized silver nanoparticles was more pronounced against Gram-negative bacteria Chromobacterium violaceum with larger zone of inhibition (18 ± 0.69 mm diameter) when compared to those from fungi. Results recommend the silver nanoparticles biosynthesized using culture filtrate of the lactic acid bacteria Lactobacillus sp. strain LCM5 for biotechnological purposes, as promising antimicrobial agents.

Biosynthesis of silver nanoparticles mediated by culture filtrate of lactic acid bacteria, characterization and antifungal activity

The EuroBiotech Journal

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Silver nanoparticles (AgNPs) are nanomaterials obtained by nanotechnology and due to their antimicrobial properties have a major importance in the control of...

Test microorganism	Inhibition zone (mm) Ag NO₃	Inhibition zone (mm) Ag NPs
Aspergillus flavus	9.00 ± 1.06c Data represent the mean of three replicates ± standard deviation; values in each column followed by the same letter are not significantly different for p<0.05 (Student test)	12.39 ± 0.61c
Aspergillus ochraceus	7.00 ± 0.37d	12.86 ± 0.78c
Penicillium expansum	11.00 ± 0.46b	15.87 ± 1.01b
Chromobacterium violaceum	16.00 ± 0.67a	18.00 ± 0.69a

Biosynthesis of silver nanoparticles mediated by culture filtrate of lactic acid bacteria, characterization and antifungal activity

Article Category: Research Article

Data publikacji: 30 kwi 2020

Zakres stron: 97 - 103

DOI: https://doi.org/10.2478/ebtj-2020-0011

Słowa kluczowe
silver nanoparticles, lactic acid bacteria, capping agent, antimicrobial activity, phytopathogenic fungi

© 2020 Adrian Matei, Sorin Matei, Gabi-Mirela Matei, Gina Cogălniceanu and Călina Petruța Cornea, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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Antimicrobial activity of silver nanoparticles

Biosynthesis of silver nanoparticles mediated by culture filtrate of lactic acid bacteria, characterization and antifungal activity

Article Category: Research Article

Data publikacji: 30 kwi 2020

Zakres stron: 97 - 103

DOI: https://doi.org/10.2478/ebtj-2020-0011

Słowa kluczowesilver nanoparticles, lactic acid bacteria, capping agent, antimicrobial activity, phytopathogenic fungi

© 2020 Adrian Matei, Sorin Matei, Gabi-Mirela Matei, Gina Cogălniceanu and Călina Petruța Cornea, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Figure 1

Figure 2

Figure 3

Figure 4

Figure 6

Figure 5

Figure 7

Antimicrobial activity of silver nanoparticles

Słowa kluczowe
silver nanoparticles, lactic acid bacteria, capping agent, antimicrobial activity, phytopathogenic fungi