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Gastroprotective effects of methanol extract of Eremomastax speciosa leaf harvested in Southern part of Nigeria in rat

Samuel O. Onoja
Samuel O. Onoja
, 
Osborn Chinagorom
Osborn Chinagorom
, 
Chinyere B. C. Ikpa
Chinyere B. C. Ikpa
, 
Kelechi G. Madubuike
Kelechi G. Madubuike
, 
Ihechiluru I. Ezeigbo
Ihechiluru I. Ezeigbo
, 
Solomon N. Ijioma
Solomon N. Ijioma
, 
Aruh O. Anaga
Aruh O. Anaga
 oraz 
Maxwell I. Ezeja
Maxwell I. Ezeja
   | 27 paź 2018
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Article Category: Research Article
Data publikacji: 27 paź 2018
Zakres stron: 200 - 208
DOI: https://doi.org/10.2478/ebtj-2018-0050
Słowa kluczowe
© 2018 Samuel O. Onoja, Osborn Chinagorom, Chinyere B. C. Ikpa, Kelechi G. Madubuike, Ihechiluru I. Ezeigbo, Solomon N. Ijioma, Aruh O. Anaga, Maxwell I. Ezeja, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Figure 1

GC-MS chromatogram of ESE.1 = benzenesulfonyl chloride, 2 = benzopyran-4-one, 5,7-dihydroxy-2-phenyl-, 3 = benzenesulfonic acid, methyl ester, 4 = 2(5H)-furanone, 4-methoxy-5-phenyl-, 5 = 4,5-dimethylthiazole, 6 = p-chlorobenzenesulfonyl chloride.
GC-MS chromatogram of ESE.1 = benzenesulfonyl chloride, 2 = benzopyran-4-one, 5,7-dihydroxy-2-phenyl-, 3 = benzenesulfonic acid, methyl ester, 4 = 2(5H)-furanone, 4-methoxy-5-phenyl-, 5 = 4,5-dimethylthiazole, 6 = p-chlorobenzenesulfonyl chloride.

Figure 2

Stomach sections of effects of ESE against indomethacin-induced gastric ulcer. Arrows indicate points of gastric ulceration. Plates A- E represents various treatments; groups A = distilled water 5.0 ml/kg, B = cimetidine 100 mg/kg, C = ESE 25 mg/kg, D = ESE 50 mg/kg, E = ESE 100 mg/kg (x 100 magnification).
Stomach sections of effects of ESE against indomethacin-induced gastric ulcer. Arrows indicate points of gastric ulceration. Plates A- E represents various treatments; groups A = distilled water 5.0 ml/kg, B = cimetidine 100 mg/kg, C = ESE 25 mg/kg, D = ESE 50 mg/kg, E = ESE 100 mg/kg (x 100 magnification).

Figure 3

The relaxant effects of ESE on isolated rabbit jejunum ex vivo ESE = Eremomastax speciosa extract, final bath concentration of ESE ranged between 57 – 456 μg/mL.
The relaxant effects of ESE on isolated rabbit jejunum ex vivo ESE = Eremomastax speciosa extract, final bath concentration of ESE ranged between 57 – 456 μg/mL.

Figure 4

Concentration-dependent effects of histamine and acetylcholine on the intrinsic contraction in isolated rabbit jejunum ex vivo HIS = histamine, ACH = acetylcholine, the final bath concentration of histamine was between 0.15 – 0.44 μg/mL while the final bath concentration of acetylcholine was between 0.015 – 0.044 μg/ml.
Concentration-dependent effects of histamine and acetylcholine on the intrinsic contraction in isolated rabbit jejunum ex vivo HIS = histamine, ACH = acetylcholine, the final bath concentration of histamine was between 0.15 – 0.44 μg/mL while the final bath concentration of acetylcholine was between 0.015 – 0.044 μg/ml.

Figure 5

The inhibitory effects of ESE on acetylcholine-induced increased intrinsic contraction of isolated rabbit jejunum ex vivo ACH = acetylcholine, ESE = Eremomastax speciosa extract, the final bath concentration of acetylcholine was 0.029 μg/mL while the final bath concentration of ESE ranged between 114 – 456 μg/mL.
The inhibitory effects of ESE on acetylcholine-induced increased intrinsic contraction of isolated rabbit jejunum ex vivo ACH = acetylcholine, ESE = Eremomastax speciosa extract, the final bath concentration of acetylcholine was 0.029 μg/mL while the final bath concentration of ESE ranged between 114 – 456 μg/mL.

Figure 6

The inhibitory effects of ESE on histamine-induced increased intrinsic contraction of isolated rabbit jejunum ex vivo HIS = histamine, ESE = Eremomastax speciosa extract, the final bath concentration of histamine was 0.29 μg/mL while the final bath concentration of ESE ranged between 114 – 456 μg/mL.
The inhibitory effects of ESE on histamine-induced increased intrinsic contraction of isolated rabbit jejunum ex vivo HIS = histamine, ESE = Eremomastax speciosa extract, the final bath concentration of histamine was 0.29 μg/mL while the final bath concentration of ESE ranged between 114 – 456 μg/mL.

Effects of ESE against indomethacin-induced gastric ulcer

GroupUlcer scoreUlcer severityUlcer index% inhibition
Distilled water, 5 ml/kg9.80 ± 1.4616.00 ± 0.8412.58 ± 0.07-
Cimetidine, 100 mg/kg5.80 ± 1.11*10.80 ± 2.8211.66 ± 0.32*7.31
ESE, 25 mg/kg4.00 ± 0.82*6.50 ± 1.55*11.05 ± 0.18*12.16
ESE, 50 mg/kg8.50 ± 1.2014.25 ± 1.8012.28 ± 0.292.38
ESE, 100 mg/kg8.00 ± 2.3115.67 ± 5.9312.37 ± 0.581.67

Chemical composition of ESE as identified by the GC-MS

S/NRT (min)%MW (g)MFChemical name
130.6040.63176C6H5ClO2SBenzenesulfonyl chloride
233.0021.25254C15H10O4Benzopyran-4-one, 5,7-dihydroxy-2-phenyl-
321.8015.63172C7H8O3SBenzenesulfonic acid, methyl ester
428.8012.50190C11H10O32(5H)-Furanone, 4-methoxy-5-phenyl-
534.504.38113C5H7NS4,5-Dimethylthiazole
632.005.63210C6H4Cl2O2Sp-Chlorobenzenesulfonyl chloride

Effects of ESE on ethanol-induced ulcer

GroupUlcer scoreUlcer severityUlcer index% inhibition
Distilled water, 5 ml/kg8.33 ± 1.6517.33 ± 3.9412.57 ± 0.55-
Misoprostol, 50 μg/kg0.50 ± 0.29*1.00 ± 0.71*5.15 ± 0.07*59.03
ESE, 25 mg/kg3.75 ± 2.259.00 ± 5.936.28 ± 0.63*50.04
ESE, 50 mg/kg7.67 ± 1.1717.67 ± 3.158.53 ± 0.40*32.14
ESE, 100 mg/kg5.00 ± 2.418.40 ± 3.617.34 ± 0.49*41.61

Effect of ESE on total gastric acidity

GroupTotal gastric acidity (mole/L)% reduction
Distilled water, 5 ml/kg0.08 ± 0.00-
Cimetidine, 100 mg/kg0.03 ± 0.01*62.5
ESE, 25 mg/kg0.06 ± 0.0025
ESE, 50 mg/kg0.04 ± 0.01*50
ESE, 100 mg/kg0.07 ± 0.0112.5
eISSN:
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Język:
Angielski
Częstotliwość wydawania:
4 razy w roku
Dziedziny czasopisma:
Life Sciences, Genetics, Biotechnology, Bioinformatics, other
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