<abstract xmlns="http://www.w3.org/1999/xhtml"><p>During menopausal transition, decreased level of estrogen brings a number of physiological problems and hormonal changes. In this study, promoter methylation of <italic>RANKL</italic> and <italic>FSHR</italic> genes were identified in 30 premenopausal and 35 postmenopausal women using methylation-specific high resolution melting (MS-HRM) analysis. The statistical analyses and their association with patient characteristics were performed by Pearson χ<sup>2</sup> and Fisher’s exact test (<italic>p</italic> &lt;0.05). The methylated <italic>RANKL</italic> gene was detected in 16 postmenopausal cases, and 12 (75.0%) of the <italic>RANKL</italic> methylated cases had hot flashes (<italic>p =</italic> 0.024). The methylated <italic>FSHR</italic> gene was detected in 18 postmenopausal cases, and 13 (75.0%) of the methylated cases had hot flashes (p = 0.028). In <italic>vitro</italic> studies demonstrated the association between <italic>RANKL</italic> expression, FSH level and hot flashes in the mouse. Although lack of epigenetic studies in this field proves our results crucial and therefore, our results showed magnitude of epigenetic profiles of Turkish Cypriot post-menopausal women. This was the first study which has investigated the <italic>RANKL</italic> and <italic>FSHR</italic> methylation and their relationship with hot flashes in postmenopausal women.</p></abstract>

During menopausal transition, decreased level of estrogen brings a number of physiological problems and hormonal changes. In this study, promoter methylation of RANKL and FSHR genes were identified in 30 premenopausal and 35 postmenopausal women using methylation-specific high resolution melting (MS-HRM) analysis. The statistical analyses and their association with patient characteristics were performed by Pearson χ2 and Fisher’s exact test (p <0.05). The methylated RANKL gene was detected in 16 postmenopausal cases, and 12 (75.0%) of the RANKL methylated cases had hot flashes (p = 0.024). The methylated FSHR gene was detected in 18 postmenopausal cases, and 13 (75.0%) of the methylated cases had hot flashes (p = 0.028). In vitro studies demonstrated the association between RANKL expression, FSH level and hot flashes in the mouse. Although lack of epigenetic studies in this field proves our results crucial and therefore, our results showed magnitude of epigenetic profiles of Turkish Cypriot post-menopausal women. This was the first study which has investigated the RANKL and FSHR methylation and their relationship with hot flashes in postmenopausal women.

RANKL is a new epigenetic biomarker for the vasomotor symptom during Menopause

Balkan Journal of Medical Genetics

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"RANKL is a new epigenetic biomarker for the vasomotor symptom during Menopause"}

During menopausal transition, decreased level of estrogen brings a number of physiological problems and hormonal changes. In this study, promoter methylation...

Genes	Postmenopause	Premenopause	Positive Hot Flashes History	Negative Hot Flashes History
RANKL (M) n	16 (45.7%)	10 (33.3%)	12 (75.0%)	4 (25.0%)
RANKL (U) n	19 (54.3%)	20 (66.7%)	7 (36.8%)	12 (63.2%)
FSHR(M) n	18 (51.4%)	20 (66.7%)	13 (72.2%)	5 (27.8%)
FSHR (U) n	17 (48.6%)	10 (33.3%)	6 (35.3%)	11 (64.7%)

Genes	Positive Hot Flashes History	Negative Hot Flashes History	p Value
RANKL (M) n	12 (75.0%)	4 (25.0%)	0.024
RANKL (U) n	7 (36.8%)	12 (63.2%)
FSHR(M) n	13 (72.2%)	5 (27.8%)	0.028
FSHR (U) n	6 (35.3%)	11 (64.7%)

RANKL is a new epigenetic biomarker for the vasomotor symptom during Menopause

Article Category: Original Article

Data publikacji: 26 sie 2020

Zakres stron: 51 - 56

DOI: https://doi.org/10.2478/bjmg-2020-0001

Słowa kluczowe
Epigenetics, genes, Hot flashes, Menopause

© 2020 Kalkan R, Altarda M, Tosun O, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

Methylation status of RANKL and FSHR genes in pre- and postmenopausal women.

Methylation status of RANKL and FSHR genes and postmenopausal women who have hot flashes.

RANKL is a new epigenetic biomarker for the vasomotor symptom during Menopause

Article Category: Original Article

Data publikacji: 26 sie 2020

Zakres stron: 51 - 56

DOI: https://doi.org/10.2478/bjmg-2020-0001

Słowa kluczoweEpigenetics, genes, Hot flashes, Menopause

© 2020 Kalkan R, Altarda M, Tosun O, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

Methylation status of RANKL and FSHR genes in pre- and postmenopausal women.

Methylation status of RANKL and FSHR genes and postmenopausal women who have hot flashes.

Słowa kluczowe
Epigenetics, genes, Hot flashes, Menopause