<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The yellow-feather broiler is a popular poultry breed in Asia, particularly in China. In this study, we performed RNA-seq analysis to identify differentially expressed genes (deGs) in the liver of yellow-feather broilers that had been subjected to acute heat stress treatment (38±1°C for 4 h, recovery 2 h) and determine the response of the liver to high temperature and its effects on yellow-feather broiler physiology. We found that the cloacal temperature and respiratory rate of yellow-feather chickens were significantly increased immediately after the initiation of acute heat stress (38°c) treatment. And after recovery for 2 h, there was no difference in the cloacal temperature and respiratory rate between the acute heat stress and control groups. A total of 834 DEGs were observed in response to heat stress by RNA-seq. Almost half of the DEGs were involved in the lipid and energy metabolism, including fatty acid metabolism (<italic>ACOX1</italic>, <italic>ACACA</italic>, <italic>ACSL1</italic>, <italic>ACSL6</italic>, <italic>ACAA1</italic>, <italic>ACAA2</italic>, <italic>HADHB</italic>, and <italic>FASN</italic>) and propanoate metabolism (<italic>ACSS2</italic>, <italic>ALDH2</italic>, <italic>ACACA</italic>, <italic>DLAT</italic>, <italic>ALDH7A1</italic>, <italic>MDH1</italic>, <italic>ME1</italic>, <italic>ABAT</italic>, <italic>SUCLG2</italic>, and <italic>ACSS3</italic>). Our findings provide the context for RNA-seq studies in the liver of yellow-feather chickens and suggest that the liver of yellow-feather broilers has the lipid and energy metabolism physiological mechanisms activated in response to heat stress.</p></abstract>

The yellow-feather broiler is a popular poultry breed in Asia, particularly in China. In this study, we performed RNA-seq analysis to identify differentially expressed genes (deGs) in the liver of yellow-feather broilers that had been subjected to acute heat stress treatment (38±1°C for 4 h, recovery 2 h) and determine the response of the liver to high temperature and its effects on yellow-feather broiler physiology. We found that the cloacal temperature and respiratory rate of yellow-feather chickens were significantly increased immediately after the initiation of acute heat stress (38°c) treatment. And after recovery for 2 h, there was no difference in the cloacal temperature and respiratory rate between the acute heat stress and control groups. A total of 834 DEGs were observed in response to heat stress by RNA-seq. Almost half of the DEGs were involved in the lipid and energy metabolism, including fatty acid metabolism (ACOX1, ACACA, ACSL1, ACSL6, ACAA1, ACAA2, HADHB, and FASN) and propanoate metabolism (ACSS2, ALDH2, ACACA, DLAT, ALDH7A1, MDH1, ME1, ABAT, SUCLG2, and ACSS3). Our findings provide the context for RNA-seq studies in the liver of yellow-feather chickens and suggest that the liver of yellow-feather broilers has the lipid and energy metabolism physiological mechanisms activated in response to heat stress.

RNA-Seq Study of Hepatic Response of Yellow-Feather Chickens to Acute Heat Stress

Department of Animal Science, Agricultural College

Guangdong Provincial Key Laboratory of Agro-animal Genomics and Molecular Breeding

Annals of Animal Science

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The yellow-feather broiler is a popular poultry breed in Asia, particularly in China. In this study, we performed RNA-seq analysis to identify differentially...

RNA-Seq Study of Hepatic Response of Yellow-Feather Chickens to Acute Heat Stress

Data publikacji: 28 sty 2020

Zakres stron: 55 - 69

Otrzymano: 09 kwi 2019

Przyjęty: 11 wrz 2019

DOI: https://doi.org/10.2478/aoas-2019-0060

Słowa kluczowe
yellow-feather chickens, liver, heat stress, RNA-seq

© 2020 Quan Zhang et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

RNA-Seq Study of Hepatic Response of Yellow-Feather Chickens to Acute Heat Stress

Data publikacji: 28 sty 2020

Zakres stron: 55 - 69

Otrzymano: 09 kwi 2019

Przyjęty: 11 wrz 2019

DOI: https://doi.org/10.2478/aoas-2019-0060

Słowa kluczoweyellow-feather chickens, liver, heat stress, RNA-seq

© 2020 Quan Zhang et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Słowa kluczowe
yellow-feather chickens, liver, heat stress, RNA-seq