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Prenatal and perinatal phthalate exposure is associated with sex-dependent changes in hippocampal miR-15b-5p and miR-34a-5p expression and changes in testicular morphology in rat offspring

Peter Štefánik
Peter Štefánik
, 
Jaroslav Michalec
Jaroslav Michalec
, 
Martina Morová
Martina Morová
, 
Lucia Olexová
Lucia Olexová
 oraz 
Lucia Kršková
Lucia Kršková
   | 30 wrz 2022
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Article Category: Original article
Data publikacji: 30 wrz 2022
Zakres stron: 191 - 199
Otrzymano: 01 mar 2022
Przyjęty: 01 wrz 2022
DOI: https://doi.org/10.2478/aiht-2022-73-3641
© 2022 Peter Štefánik, Jaroslav Michalec, Martina Morová, Lucia Olexová, and Lucia Kršková, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1

Comparison of luminal area cryosections of testes stained with Gill’s haematoxylin and eosin between a control and a phthalate-treated male rat
Comparison of luminal area cryosections of testes stained with Gill’s haematoxylin and eosin between a control and a phthalate-treated male rat

Figure 2

Comparison of representative micrographs of miR-15b-5p positive signals (green) in the hippocampal cornus ammonis 2 (CA2) region between a control and a phthalate-treated female rat
Comparison of representative micrographs of miR-15b-5p positive signals (green) in the hippocampal cornus ammonis 2 (CA2) region between a control and a phthalate-treated female rat

Figure 3

Relative number of miR-15b-5p-positive neurons in the hippocampal cornus ammonis 1 (CA1) region of control male rats (Ctrl m; n=5), control female rats (Ctrl f; n=9), phthalate-treated male rats (Pht m; n=5), and phthalate-treated female rats (Pht f; n=10). Sex × treatment interaction is significant (F1,25=8.3501, p=0.0078). ** p<0.01 and # p<0.05 vs respective male and female controls (unpaired Student’s t-test: t=-2.778, p=0.017)
Relative number of miR-15b-5p-positive neurons in the hippocampal cornus ammonis 1 (CA1) region of control male rats (Ctrl m; n=5), control female rats (Ctrl f; n=9), phthalate-treated male rats (Pht m; n=5), and phthalate-treated female rats (Pht f; n=10). Sex × treatment interaction is significant (F1,25=8.3501, p=0.0078). ** p<0.01 and # p<0.05 vs respective male and female controls (unpaired Student’s t-test: t=-2.778, p=0.017)

Figure 4

Relative number of miR-15b-5p-positive neurons in the hippocampal cornus ammonis 2 (CA2) region of control male rats (Ctrl m; n=5), control female rats (Ctrl f; n=9), phthalate-treated male rats (Pht m; n=5), and phthalate-treated female rats (Pht f; n=10). The sex effect (F1,25=4.9967, p=0.0345) and treatment effect (F1,25=4.7352, p=0.0392) are significant. * p<0.05 and # p<0.05 for the Ctrl m and Ctrl f comparison (unpaired Student’s t-test: t=-2.459, p=0.03)
Relative number of miR-15b-5p-positive neurons in the hippocampal cornus ammonis 2 (CA2) region of control male rats (Ctrl m; n=5), control female rats (Ctrl f; n=9), phthalate-treated male rats (Pht m; n=5), and phthalate-treated female rats (Pht f; n=10). The sex effect (F1,25=4.9967, p=0.0345) and treatment effect (F1,25=4.7352, p=0.0392) are significant. * p<0.05 and # p<0.05 for the Ctrl m and Ctrl f comparison (unpaired Student’s t-test: t=-2.459, p=0.03)

Figure 5

Relative number of miR-15b-5p-positive neurons in the hippocampal cornus ammonis 3 (CA3) region of control male rats (Ctrl m; n=5), control female rats (Ctrl f; n=9), phthalate-treated male rats (Pht m; n=5), and phthalate-treated female rats (Pht f; n=10). The sex effect shows a trend towards significance (F1,25=4.0915, p=0.0539). $ p<0.05 for the Ctrl f and Pht f comparison (unpaired Student’s t-test: t=-2.529, p=0.022); # p<0.05 for the Ctrl m and Ctrl f comparison (unpaired Student’s t-test: t=-2.758, p=0.017)
Relative number of miR-15b-5p-positive neurons in the hippocampal cornus ammonis 3 (CA3) region of control male rats (Ctrl m; n=5), control female rats (Ctrl f; n=9), phthalate-treated male rats (Pht m; n=5), and phthalate-treated female rats (Pht f; n=10). The sex effect shows a trend towards significance (F1,25=4.0915, p=0.0539). $ p<0.05 for the Ctrl f and Pht f comparison (unpaired Student’s t-test: t=-2.529, p=0.022); # p<0.05 for the Ctrl m and Ctrl f comparison (unpaired Student’s t-test: t=-2.758, p=0.017)

Figure 6

Relative number of miR-15b-5p-positive neurons in the dentate gyrus (DG) of control male rats (Ctrl m; n=5), control female rats (Ctrl f; n=9), phthalate-treated male rats (Pht m; n=5), and phthalate-treated female rats (Pht f; n=10). ## p<0.01 for the Ctrl f and Pht f comparison (unpaired Student’s t-test: t=-3.143, p=0.008)
Relative number of miR-15b-5p-positive neurons in the dentate gyrus (DG) of control male rats (Ctrl m; n=5), control female rats (Ctrl f; n=9), phthalate-treated male rats (Pht m; n=5), and phthalate-treated female rats (Pht f; n=10). ## p<0.01 for the Ctrl f and Pht f comparison (unpaired Student’s t-test: t=-3.143, p=0.008)

Figure 7

Relative number of miR-34a-5p-positive neurons in the hippocampal cornus ammonis 1 (CA1) region of control male rats (Ctrl m; n=4), control female rats (Ctrl f; n=8), phthalate-treated male rats (Pht m; n=4), and phthalate-treated female rats (Pht f; n=7)
Relative number of miR-34a-5p-positive neurons in the hippocampal cornus ammonis 1 (CA1) region of control male rats (Ctrl m; n=4), control female rats (Ctrl f; n=8), phthalate-treated male rats (Pht m; n=4), and phthalate-treated female rats (Pht f; n=7)

Figure 8

Relative number of miR-34a-5p-positive neurons in the hippocampal cornus ammonis 2 (CA2) region of control male rats (Ctrl m; n=4), control female rats (Ctrl f; n=8), phthalate-treated male rats (Pht m; n=4), and phthalate-treated female rats (Pht f; n=7)
Relative number of miR-34a-5p-positive neurons in the hippocampal cornus ammonis 2 (CA2) region of control male rats (Ctrl m; n=4), control female rats (Ctrl f; n=8), phthalate-treated male rats (Pht m; n=4), and phthalate-treated female rats (Pht f; n=7)

Figure 9

Relative number of miR-34a-5p-positive neurons in the hippocampal cornus ammonis 3 (CA3) region of control male rats (Ctrl m; n=4), control female rats (Ctrl f; n=8), phthalate-treated male rats (Pht m; n=4), and phthalate-treated female rats (Pht f; n=7). The treatment effect (F1,19=5.009, p=0.037) and the sex × treatment interaction (F1,19=4.39, p=0.049) are significant. ** p<0.001 and # p<0.05 for the Pht m and Pht f comparison (unpaired Student’s t-test: t=2.363, p=0.042)
Relative number of miR-34a-5p-positive neurons in the hippocampal cornus ammonis 3 (CA3) region of control male rats (Ctrl m; n=4), control female rats (Ctrl f; n=8), phthalate-treated male rats (Pht m; n=4), and phthalate-treated female rats (Pht f; n=7). The treatment effect (F1,19=5.009, p=0.037) and the sex × treatment interaction (F1,19=4.39, p=0.049) are significant. ** p<0.001 and # p<0.05 for the Pht m and Pht f comparison (unpaired Student’s t-test: t=2.363, p=0.042)

Figure 10

Relative number of miR-34a-5p-positive neurons in the dentate gyrus (DG) of control male rats (Ctrl m; n=4), control female rats (Ctrl f; n=8), phthalate-treated male rats (Pht m; n=4), and phthalate-treated female rats (Pht f; n=7). # p<0.05 for the Ctrl f and Pht f comparison (unpaired Student’s t-test: t=-2.476, p=0.028)
Relative number of miR-34a-5p-positive neurons in the dentate gyrus (DG) of control male rats (Ctrl m; n=4), control female rats (Ctrl f; n=8), phthalate-treated male rats (Pht m; n=4), and phthalate-treated female rats (Pht f; n=7). # p<0.05 for the Ctrl f and Pht f comparison (unpaired Student’s t-test: t=-2.476, p=0.028)

Comparison of sperm-producing duct areas (μm2) between control (n=12) and phthalate-treated (n=12) male rats. Values are presented as means±standard deviations (SD)

Items Control (Mean±SD) Phthalates (Mean±SD) p value
Channel area (μm2) 100292±3768.6 103586±5053.5 0.595936
Inner duct area (μm2) 19419±1769 25151±1433.3 0.019613
Inner duct area / channel area (μm2) 188091±1430 241257±1130 0.008059
eISSN:
1848-6312
Języki:
Angielski, Slovenian
Częstotliwość wydawania:
4 razy w roku
Dziedziny czasopisma:
Medicine, Basic Medical Science, other
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