Accesso libero

Prenatal and perinatal phthalate exposure is associated with sex-dependent changes in hippocampal miR-15b-5p and miR-34a-5p expression and changes in testicular morphology in rat offspring

Peter Štefánik

Peter Štefánik

Comenius University in Bratislava Faculty of Natural Sciences, Department of Animal Physiology and EthologyBratislava, Slovakia
Cerca questo autore su
Sciendo|Google Scholar
Štefánik, Peter
, 
Jaroslav Michalec

Jaroslav Michalec

Comenius University in Bratislava Faculty of Natural Sciences, Department of Animal Physiology and EthologyBratislava, Slovakia
Cerca questo autore su
Sciendo|Google Scholar
Michalec, Jaroslav
, 
Martina Morová

Martina Morová

Comenius University in Bratislava Faculty of Natural Sciences, Department of Animal Physiology and EthologyBratislava, Slovakia
Cerca questo autore su
Sciendo|Google Scholar
Morová, Martina
, 
Lucia Olexová

Lucia Olexová

Comenius University in Bratislava Faculty of Natural Sciences, Department of Animal Physiology and EthologyBratislava, Slovakia
Cerca questo autore su
Sciendo|Google Scholar
Olexová, Lucia
 e 
Lucia Kršková

Lucia Kršková

Comenius University in Bratislava Faculty of Natural Sciences, Department of Animal Physiology and EthologyBratislava, Slovakia
Cerca questo autore su
Sciendo|Google Scholar
Kršková, Lucia
  
30 set 2022
Prenatal and perinatal phthalate exposure is associated with sex-dependent changes in hippocampal miR-15b-5p and miR-34a-5p expression and changes in testicular morphology in rat offspring's Cover Image
INFORMAZIONI SU QUESTO ARTICOLO
Articolo precedente
Articolo Successivo
Cita
Scarica la copertina
Categoria dell'articolo: Original article
Pubblicato online: 30 set 2022
Pagine: 191 - 199
Ricevuto: 01 mar 2022
Accettato: 01 set 2022
DOI: https://doi.org/10.2478/aiht-2022-73-3641
Parole chiave
© 2022 Peter Štefánik, Jaroslav Michalec, Martina Morová, Lucia Olexová, and Lucia Kršková, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1

Comparison of luminal area cryosections of testes stained with Gill’s haematoxylin and eosin between a control and a phthalate-treated male rat
Comparison of luminal area cryosections of testes stained with Gill’s haematoxylin and eosin between a control and a phthalate-treated male rat

Figure 2

Comparison of representative micrographs of miR-15b-5p positive signals (green) in the hippocampal cornus ammonis 2 (CA2) region between a control and a phthalate-treated female rat
Comparison of representative micrographs of miR-15b-5p positive signals (green) in the hippocampal cornus ammonis 2 (CA2) region between a control and a phthalate-treated female rat

Figure 3

Relative number of miR-15b-5p-positive neurons in the hippocampal cornus ammonis 1 (CA1) region of control male rats (Ctrl m; n=5), control female rats (Ctrl f; n=9), phthalate-treated male rats (Pht m; n=5), and phthalate-treated female rats (Pht f; n=10). Sex × treatment interaction is significant (F1,25=8.3501, p=0.0078). ** p<0.01 and # p<0.05 vs respective male and female controls (unpaired Student’s t-test: t=-2.778, p=0.017)
Relative number of miR-15b-5p-positive neurons in the hippocampal cornus ammonis 1 (CA1) region of control male rats (Ctrl m; n=5), control female rats (Ctrl f; n=9), phthalate-treated male rats (Pht m; n=5), and phthalate-treated female rats (Pht f; n=10). Sex × treatment interaction is significant (F1,25=8.3501, p=0.0078). ** p<0.01 and # p<0.05 vs respective male and female controls (unpaired Student’s t-test: t=-2.778, p=0.017)

Figure 4

Relative number of miR-15b-5p-positive neurons in the hippocampal cornus ammonis 2 (CA2) region of control male rats (Ctrl m; n=5), control female rats (Ctrl f; n=9), phthalate-treated male rats (Pht m; n=5), and phthalate-treated female rats (Pht f; n=10). The sex effect (F1,25=4.9967, p=0.0345) and treatment effect (F1,25=4.7352, p=0.0392) are significant. * p<0.05 and # p<0.05 for the Ctrl m and Ctrl f comparison (unpaired Student’s t-test: t=-2.459, p=0.03)
Relative number of miR-15b-5p-positive neurons in the hippocampal cornus ammonis 2 (CA2) region of control male rats (Ctrl m; n=5), control female rats (Ctrl f; n=9), phthalate-treated male rats (Pht m; n=5), and phthalate-treated female rats (Pht f; n=10). The sex effect (F1,25=4.9967, p=0.0345) and treatment effect (F1,25=4.7352, p=0.0392) are significant. * p<0.05 and # p<0.05 for the Ctrl m and Ctrl f comparison (unpaired Student’s t-test: t=-2.459, p=0.03)

Figure 5

Relative number of miR-15b-5p-positive neurons in the hippocampal cornus ammonis 3 (CA3) region of control male rats (Ctrl m; n=5), control female rats (Ctrl f; n=9), phthalate-treated male rats (Pht m; n=5), and phthalate-treated female rats (Pht f; n=10). The sex effect shows a trend towards significance (F1,25=4.0915, p=0.0539). $ p<0.05 for the Ctrl f and Pht f comparison (unpaired Student’s t-test: t=-2.529, p=0.022); # p<0.05 for the Ctrl m and Ctrl f comparison (unpaired Student’s t-test: t=-2.758, p=0.017)
Relative number of miR-15b-5p-positive neurons in the hippocampal cornus ammonis 3 (CA3) region of control male rats (Ctrl m; n=5), control female rats (Ctrl f; n=9), phthalate-treated male rats (Pht m; n=5), and phthalate-treated female rats (Pht f; n=10). The sex effect shows a trend towards significance (F1,25=4.0915, p=0.0539). $ p<0.05 for the Ctrl f and Pht f comparison (unpaired Student’s t-test: t=-2.529, p=0.022); # p<0.05 for the Ctrl m and Ctrl f comparison (unpaired Student’s t-test: t=-2.758, p=0.017)

Figure 6

Relative number of miR-15b-5p-positive neurons in the dentate gyrus (DG) of control male rats (Ctrl m; n=5), control female rats (Ctrl f; n=9), phthalate-treated male rats (Pht m; n=5), and phthalate-treated female rats (Pht f; n=10). ## p<0.01 for the Ctrl f and Pht f comparison (unpaired Student’s t-test: t=-3.143, p=0.008)
Relative number of miR-15b-5p-positive neurons in the dentate gyrus (DG) of control male rats (Ctrl m; n=5), control female rats (Ctrl f; n=9), phthalate-treated male rats (Pht m; n=5), and phthalate-treated female rats (Pht f; n=10). ## p<0.01 for the Ctrl f and Pht f comparison (unpaired Student’s t-test: t=-3.143, p=0.008)

Figure 7

Relative number of miR-34a-5p-positive neurons in the hippocampal cornus ammonis 1 (CA1) region of control male rats (Ctrl m; n=4), control female rats (Ctrl f; n=8), phthalate-treated male rats (Pht m; n=4), and phthalate-treated female rats (Pht f; n=7)
Relative number of miR-34a-5p-positive neurons in the hippocampal cornus ammonis 1 (CA1) region of control male rats (Ctrl m; n=4), control female rats (Ctrl f; n=8), phthalate-treated male rats (Pht m; n=4), and phthalate-treated female rats (Pht f; n=7)

Figure 8

Relative number of miR-34a-5p-positive neurons in the hippocampal cornus ammonis 2 (CA2) region of control male rats (Ctrl m; n=4), control female rats (Ctrl f; n=8), phthalate-treated male rats (Pht m; n=4), and phthalate-treated female rats (Pht f; n=7)
Relative number of miR-34a-5p-positive neurons in the hippocampal cornus ammonis 2 (CA2) region of control male rats (Ctrl m; n=4), control female rats (Ctrl f; n=8), phthalate-treated male rats (Pht m; n=4), and phthalate-treated female rats (Pht f; n=7)

Figure 9

Relative number of miR-34a-5p-positive neurons in the hippocampal cornus ammonis 3 (CA3) region of control male rats (Ctrl m; n=4), control female rats (Ctrl f; n=8), phthalate-treated male rats (Pht m; n=4), and phthalate-treated female rats (Pht f; n=7). The treatment effect (F1,19=5.009, p=0.037) and the sex × treatment interaction (F1,19=4.39, p=0.049) are significant. ** p<0.001 and # p<0.05 for the Pht m and Pht f comparison (unpaired Student’s t-test: t=2.363, p=0.042)
Relative number of miR-34a-5p-positive neurons in the hippocampal cornus ammonis 3 (CA3) region of control male rats (Ctrl m; n=4), control female rats (Ctrl f; n=8), phthalate-treated male rats (Pht m; n=4), and phthalate-treated female rats (Pht f; n=7). The treatment effect (F1,19=5.009, p=0.037) and the sex × treatment interaction (F1,19=4.39, p=0.049) are significant. ** p<0.001 and # p<0.05 for the Pht m and Pht f comparison (unpaired Student’s t-test: t=2.363, p=0.042)

Figure 10

Relative number of miR-34a-5p-positive neurons in the dentate gyrus (DG) of control male rats (Ctrl m; n=4), control female rats (Ctrl f; n=8), phthalate-treated male rats (Pht m; n=4), and phthalate-treated female rats (Pht f; n=7). # p<0.05 for the Ctrl f and Pht f comparison (unpaired Student’s t-test: t=-2.476, p=0.028)
Relative number of miR-34a-5p-positive neurons in the dentate gyrus (DG) of control male rats (Ctrl m; n=4), control female rats (Ctrl f; n=8), phthalate-treated male rats (Pht m; n=4), and phthalate-treated female rats (Pht f; n=7). # p<0.05 for the Ctrl f and Pht f comparison (unpaired Student’s t-test: t=-2.476, p=0.028)

Comparison of sperm-producing duct areas (μm2) between control (n=12) and phthalate-treated (n=12) male rats_ Values are presented as means±standard deviations (SD)

Items Control (Mean±SD) Phthalates (Mean±SD) p value
Channel area (μm2) 100292±3768.6 103586±5053.5 0.595936
Inner duct area (μm2) 19419±1769 25151±1433.3 0.019613
Inner duct area / channel area (μm2) 188091±1430 241257±1130 0.008059
Lingue:
Inglese, Croatian, Slovenian
Frequenza di pubblicazione:
4 volte all'anno
Argomenti della rivista:
Medicina, Scienze medicali di base, Scienze medicali di base, altro
Feed RSS della rivista