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Figure 1
Kidney absolute and relative kidney weights in the experimental groups. Bars represent means ±SEM. Bars with different letters differ significantly from each other (p<0.05). A – absolute kidney weight B– relative kidney weight
Figure 2
Histopathological analysis of the renal cortex in the experimental groups. Narrowing in the Bowman’s space, renal corpuscular atrophy (white arrow), peritubular vascular congestion, oedema, desquamation of epithelial cells (black arrow) in the A group and necrotic tubules (dashed area) in the ASeD group. A– control; B– Se-supplemented (SeS) group; C– Se-deficient (SeD) group; D – A1254-treated (A) group; E– Se-supplemented A1254-treated (ASeS) group; F– Se-deficient A1254-treated (ASeD) group
Figure 3
Histopathological analysis of the renal medulla in the experimental groups. Denudation of renal tubular cells (black arrow) in the A group. A– control; B– Se-supplemented (SeS) group; C– Se-deficient (SeD) group; D– A1254-treated (A) group; E – Se-supplemented A1254-treated (ASeS) group; F– Se-deficient A1254-treated (ASeD) group
Figure 4
Ultrastructure of glomeruli in the experimental groups. Apoptotic (white arrow), endothelial (e), and mesangial (m) cells in the SeD group. Lipid droplets (arrow head) and lysosomes (black arrow) in the cytoplasm of podocytes and apoptotic cells (white arrow) in the A and ASeD groups. Regular pedicels (white arrow), effacement of the pedicels (black arrow) in the ASeS group. p – podocyte; m – mesangial cell; e – endothelial cell; A – control; B – Se-supplemented (SeS) group; C – Se-deficient (SeD) group; D – A1254-treated (A) group; E – Se-supplemented A1254-treated (ASeS) group; F – Se-deficient A1254-treated (ASeD) group
Figure 5
Ultrastructure of tubules in experimental groups. Lysosomes (white arrow) and karyolitic nuclei (black arrow) in the SeD group. Lysosomes (white arrow), apoptotic cells (black arrow head), and loss of microvilli in the A1254 group. Degeneration of tubular epithelial cells in the ASeD group. PT – proximal tubule; DT – distal tubule; m – mitochondria; mv – microvilli; A– control; B – Se-supplemented (SeS) group; C – Se-deficient (SeD) group; D – A1254-treated (A) group; E – Se-supplemented A1254-treated (ASeS) group; F – Se-deficient A1254-treated (ASeD) group
Figure 6
TUNEL assay of the renal cortex and apoptotic cell counts in the experimental groups. Positive nuclei for apoptosis are labeled pink. Arrows indicate TUNEL-positive (apoptotic) cells (400x magnification). Bars represent means ±SEM. Bars with different letters differ significantly from each other (p<0.05). A– control; B – Se-supplemented (SeS) group; C – Se-deficient (SeD) group; D – A1254-treated (A) group; E– Se-supplemented A1254-treated (ASeS) group; F – Se-deficient A1254-treated (ASeD) group
Figure 7
TUNEL assay of the renal medulla in experimental groups. Positive nuclei for apoptosis are labeled pink by the chromogenic reaction of Fast Red substrate by alkaline phosphatase (400x magnification).Arrows indicate TUNEL-positive (apoptotic) cells. Bars represent means ±SEM. Bars with different letters differ significantly from each other (p<0.05). A– control; B – Se-supplemented (SeS) group; C – Se-deficient (SeD) group; D – A1254-treated (A) group; E – Se-supplemented A1254-treated (ASeS) group; F – Se-deficient A1254-treated (ASeD) group
Figure 8
Antioxidant enzyme activities in the experimental groups. Bars represent means ±SEM. Bars with different letters differ significantly from each other (p<0.05). A – SOD activities; B – CAT activities; C – GPx1 activities SOD – superoxide dismutase; CAT – catalase; GPx1 – glutathione peroxidase; Se-supplemented (SeS) group; Se-deficient (SeD) group; A1254-treated (A) group; Se-supplemented A1254-treated (ASeS) group; Se-deficient A1254-treated (ASeD) group
Figure 9
TAOC (A), MDA (B), carbonyl group (C) and total GSH (D) levels in the study groups. Bars represent means ±SEM. Bars with different letters differ significantly from each other (p<0.05). MDA – malondialdehyde; TAOC – total antioxidant capacity; Se-supplemented (SeS) group; Se-deficient (SeD) group; A1254-treated (A) group; Se-supplemented A1254-treated (ASeS) group; Se-deficient A1254-treated (ASeD) group