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Renal changes and apoptosis caused by subacute exposure to Aroclor 1254 in selenium-deficient and selenium-supplemented rats

Naciye Dilara Zeybek

Naciye Dilara Zeybek

Hacettepe University Faculty of Medicine, Department of Histology and EmbryologyAnkara, Turkey
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Zeybek, Naciye Dilara
, 
Ünzile Sur

Ünzile Sur

  • Hacettepe University Faculty of Pharmacy, Department of ToxicologyAnkara, Turkey
  • Atatürk University Faculty of Pharmacy, Department of ToxicologyErzurum, Turkey
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Sur, Ünzile
, 
Ofcan Oflaz

Ofcan Oflaz

Hacettepe University Faculty of Science, Department of BiologyAnkara, Turkey
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Oflaz, Ofcan
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Pınar Erkekoğlu

Pınar Erkekoğlu

Hacettepe University Faculty of Pharmacy, Department of ToxicologyAnkara, Turkey
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Erkekoğlu, Pınar
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Aylin Balcı

Aylin Balcı

Hacettepe University Faculty of Pharmacy, Department of ToxicologyAnkara, Turkey
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Balcı, Aylin
, 
Gizem Özkemahlı

Gizem Özkemahlı

Erzincan Binali Yıldırım University Faculty of Pharmacy, Department of ToxicologyErzincan, Turkey
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Özkemahlı, Gizem
, 
Ali Aşçı

Ali Aşçı

Atatürk University Faculty of Pharmacy, Department of ToxicologyErzurum, Turkey
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Aşçı, Ali
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Murat Kızılgün

Murat Kızılgün

Dışkapı Children’s Health and Diseases, Haematology, and Oncology Training and Research HospitalAnkara, Turkey
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Kızılgün, Murat
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Oğuz Han Edebal

Oğuz Han Edebal

Dışkapı Children’s Health and Diseases, Haematology, and Oncology Training and Research HospitalAnkara, Turkey
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Edebal, Oğuz Han
 und 
Belma Koçer-Gümüşel

Belma Koçer-Gümüşel

Lokman Hekim University Faculty of Pharmacy, Department of ToxicologyAnkara, Turkey
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Koçer-Gümüşel, Belma
  
29. Juni 2020
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Artikel-Kategorie: Original article
Online veröffentlicht: 29. Juni 2020
Seitenbereich: 110 - 120
Eingereicht: 01. Nov. 2019
Akzeptiert: 01. Juni 2020
DOI: https://doi.org/10.2478/aiht-2020-71-3360
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© 2020 Naciye Dilara Zeybek et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1

Kidney absolute and relative kidney weights in the experimental groups. Bars represent means ±SEM. Bars with different letters differ significantly from each other (p<0.05). A – absolute kidney weight B– relative kidney weight
Kidney absolute and relative kidney weights in the experimental groups. Bars represent means ±SEM. Bars with different letters differ significantly from each other (p<0.05). A – absolute kidney weight B– relative kidney weight

Figure 2

Histopathological analysis of the renal cortex in the experimental groups. Narrowing in the Bowman’s space, renal corpuscular atrophy (white arrow), peritubular vascular congestion, oedema, desquamation of epithelial cells (black arrow) in the A group and necrotic tubules (dashed area) in the ASeD group. A– control; B– Se-supplemented (SeS) group; C– Se-deficient (SeD) group; D – A1254-treated (A) group; E– Se-supplemented A1254-treated (ASeS) group; F– Se-deficient A1254-treated (ASeD) group
Histopathological analysis of the renal cortex in the experimental groups. Narrowing in the Bowman’s space, renal corpuscular atrophy (white arrow), peritubular vascular congestion, oedema, desquamation of epithelial cells (black arrow) in the A group and necrotic tubules (dashed area) in the ASeD group. A– control; B– Se-supplemented (SeS) group; C– Se-deficient (SeD) group; D – A1254-treated (A) group; E– Se-supplemented A1254-treated (ASeS) group; F– Se-deficient A1254-treated (ASeD) group

Figure 3

Histopathological analysis of the renal medulla in the experimental groups. Denudation of renal tubular cells (black arrow) in the A group. A– control; B– Se-supplemented (SeS) group; C– Se-deficient (SeD) group; D– A1254-treated (A) group; E – Se-supplemented A1254-treated (ASeS) group; F– Se-deficient A1254-treated (ASeD) group
Histopathological analysis of the renal medulla in the experimental groups. Denudation of renal tubular cells (black arrow) in the A group. A– control; B– Se-supplemented (SeS) group; C– Se-deficient (SeD) group; D– A1254-treated (A) group; E – Se-supplemented A1254-treated (ASeS) group; F– Se-deficient A1254-treated (ASeD) group

Figure 4

Ultrastructure of glomeruli in the experimental groups. Apoptotic (white arrow), endothelial (e), and mesangial (m) cells in the SeD group. Lipid droplets (arrow head) and lysosomes (black arrow) in the cytoplasm of podocytes and apoptotic cells (white arrow) in the A and ASeD groups. Regular pedicels (white arrow), effacement of the pedicels (black arrow) in the ASeS group. p – podocyte; m – mesangial cell; e – endothelial cell; A – control; B – Se-supplemented (SeS) group; C – Se-deficient (SeD) group; D – A1254-treated (A) group; E – Se-supplemented A1254-treated (ASeS) group; F – Se-deficient A1254-treated (ASeD) group
Ultrastructure of glomeruli in the experimental groups. Apoptotic (white arrow), endothelial (e), and mesangial (m) cells in the SeD group. Lipid droplets (arrow head) and lysosomes (black arrow) in the cytoplasm of podocytes and apoptotic cells (white arrow) in the A and ASeD groups. Regular pedicels (white arrow), effacement of the pedicels (black arrow) in the ASeS group. p – podocyte; m – mesangial cell; e – endothelial cell; A – control; B – Se-supplemented (SeS) group; C – Se-deficient (SeD) group; D – A1254-treated (A) group; E – Se-supplemented A1254-treated (ASeS) group; F – Se-deficient A1254-treated (ASeD) group

Figure 5

Ultrastructure of tubules in experimental groups. Lysosomes (white arrow) and karyolitic nuclei (black arrow) in the SeD group. Lysosomes (white arrow), apoptotic cells (black arrow head), and loss of microvilli in the A1254 group. Degeneration of tubular epithelial cells in the ASeD group. PT – proximal tubule; DT – distal tubule; m – mitochondria; mv – microvilli; A– control; B – Se-supplemented (SeS) group; C – Se-deficient (SeD) group; D – A1254-treated (A) group; E – Se-supplemented A1254-treated (ASeS) group; F – Se-deficient A1254-treated (ASeD) group
Ultrastructure of tubules in experimental groups. Lysosomes (white arrow) and karyolitic nuclei (black arrow) in the SeD group. Lysosomes (white arrow), apoptotic cells (black arrow head), and loss of microvilli in the A1254 group. Degeneration of tubular epithelial cells in the ASeD group. PT – proximal tubule; DT – distal tubule; m – mitochondria; mv – microvilli; A– control; B – Se-supplemented (SeS) group; C – Se-deficient (SeD) group; D – A1254-treated (A) group; E – Se-supplemented A1254-treated (ASeS) group; F – Se-deficient A1254-treated (ASeD) group

Figure 6

TUNEL assay of the renal cortex and apoptotic cell counts in the experimental groups. Positive nuclei for apoptosis are labeled pink. Arrows indicate TUNEL-positive (apoptotic) cells (400x magnification). Bars represent means ±SEM. Bars with different letters differ significantly from each other (p<0.05). A– control; B – Se-supplemented (SeS) group; C – Se-deficient (SeD) group; D – A1254-treated (A) group; E– Se-supplemented A1254-treated (ASeS) group; F – Se-deficient A1254-treated (ASeD) group
TUNEL assay of the renal cortex and apoptotic cell counts in the experimental groups. Positive nuclei for apoptosis are labeled pink. Arrows indicate TUNEL-positive (apoptotic) cells (400x magnification). Bars represent means ±SEM. Bars with different letters differ significantly from each other (p<0.05). A– control; B – Se-supplemented (SeS) group; C – Se-deficient (SeD) group; D – A1254-treated (A) group; E– Se-supplemented A1254-treated (ASeS) group; F – Se-deficient A1254-treated (ASeD) group

Figure 7

TUNEL assay of the renal medulla in experimental groups. Positive nuclei for apoptosis are labeled pink by the chromogenic reaction of Fast Red substrate by alkaline phosphatase (400x magnification).Arrows indicate TUNEL-positive (apoptotic) cells. Bars represent means ±SEM. Bars with different letters differ significantly from each other (p<0.05). A– control; B – Se-supplemented (SeS) group; C – Se-deficient (SeD) group; D – A1254-treated (A) group; E – Se-supplemented A1254-treated (ASeS) group; F – Se-deficient A1254-treated (ASeD) group
TUNEL assay of the renal medulla in experimental groups. Positive nuclei for apoptosis are labeled pink by the chromogenic reaction of Fast Red substrate by alkaline phosphatase (400x magnification).Arrows indicate TUNEL-positive (apoptotic) cells. Bars represent means ±SEM. Bars with different letters differ significantly from each other (p<0.05). A– control; B – Se-supplemented (SeS) group; C – Se-deficient (SeD) group; D – A1254-treated (A) group; E – Se-supplemented A1254-treated (ASeS) group; F – Se-deficient A1254-treated (ASeD) group

Figure 8

Antioxidant enzyme activities in the experimental groups. Bars represent means ±SEM. Bars with different letters differ significantly from each other (p<0.05). A – SOD activities; B – CAT activities; C – GPx1 activities SOD – superoxide dismutase; CAT – catalase; GPx1 – glutathione peroxidase; Se-supplemented (SeS) group; Se-deficient (SeD) group; A1254-treated (A) group; Se-supplemented A1254-treated (ASeS) group; Se-deficient A1254-treated (ASeD) group
Antioxidant enzyme activities in the experimental groups. Bars represent means ±SEM. Bars with different letters differ significantly from each other (p<0.05). A – SOD activities; B – CAT activities; C – GPx1 activities SOD – superoxide dismutase; CAT – catalase; GPx1 – glutathione peroxidase; Se-supplemented (SeS) group; Se-deficient (SeD) group; A1254-treated (A) group; Se-supplemented A1254-treated (ASeS) group; Se-deficient A1254-treated (ASeD) group

Figure 9

TAOC (A), MDA (B), carbonyl group (C) and total GSH (D) levels in the study groups. Bars represent means ±SEM. Bars with different letters differ significantly from each other (p<0.05). MDA – malondialdehyde; TAOC – total antioxidant capacity; Se-supplemented (SeS) group; Se-deficient (SeD) group; A1254-treated (A) group; Se-supplemented A1254-treated (ASeS) group; Se-deficient A1254-treated (ASeD) group
TAOC (A), MDA (B), carbonyl group (C) and total GSH (D) levels in the study groups. Bars represent means ±SEM. Bars with different letters differ significantly from each other (p<0.05). MDA – malondialdehyde; TAOC – total antioxidant capacity; Se-supplemented (SeS) group; Se-deficient (SeD) group; A1254-treated (A) group; Se-supplemented A1254-treated (ASeS) group; Se-deficient A1254-treated (ASeD) group
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