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Study of the expression of genes associated with post-translational changes in histones in the internal thoracic artery and the saphenous vein grafts used in coronary artery bypass grafting procedure

Sandra Kałużna

Sandra Kałużna

Department of Histology and Embryology, Poznań University of Medical SciencesPoznań, Poland
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Kałużna, Sandra
, 
Mariusz J. Nawrocki

Mariusz J. Nawrocki

Department of Anatomy, Poznań University of Medical SciencesPoznań, Poland
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Nawrocki, Mariusz J.
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Rut Bryl

Rut Bryl

Department of Anatomy, Poznań University of Medical SciencesPoznań, Poland
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Bryl, Rut
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Katarzyna Stefańska

Katarzyna Stefańska

Department of Histology and Embryology, Poznań University of Medical SciencesPoznań, Poland
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Stefańska, Katarzyna
, 
Marek Jemielity

Marek Jemielity

Department of Cardiac Surgery and Transplantology, Poznań University of Medical SciencesPoznań, Poland
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Jemielity, Marek
, 
Paul Mozdziak

Paul Mozdziak

Physiology Graduate Program, North Carolina State UniversityNC, USA
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Mozdziak, Paul
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Michał Nowicki

Michał Nowicki

Department of Histology and Embryology, Poznań University of Medical SciencesPoznań, Poland
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Nowicki, Michał
 oraz 
Bartłomiej Perek

Bartłomiej Perek

Department of Cardiac Surgery and Transplantology, Poznań University of Medical SciencesPoznań, Poland
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Perek, Bartłomiej
  
31 gru 2020
Study of the expression of genes associated with post-translational changes in histones in the internal thoracic artery and the saphenous vein grafts used in coronary artery bypass grafting procedure's Cover Image
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Data publikacji: 31 gru 2020
Zakres stron: 183 - 189
Otrzymano: 09 lis 2020
Przyjęty: 19 gru 2020
DOI: https://doi.org/10.2478/acb-2020-0024
Słowa kluczowe
© 2020 Sandra Kałużna, Mariusz J. Nawrocki, Rut Bryl, Katarzyna Stefańska, Marek Jemielity, Paul Mozdziak, Michał Nowicki, Bartłomiej Perek, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

Change in the expression of genes: JHDM1D, PHF8, HDAC1, HDAC2 and HDAC3 in the saphenous vein in relation to the expression in the internal thoracic artery - vessels taken from patients who underwent CABG surgery. Total RNA was reverse transcribed and cDNA was analyzed by relative quantification by RT-qPCR. JHDM1D, PHF8, HDAC1, HDAC2 and HDAC3 mRNA levels were adjusted for PBGD and ACTB levels
Change in the expression of genes: JHDM1D, PHF8, HDAC1, HDAC2 and HDAC3 in the saphenous vein in relation to the expression in the internal thoracic artery - vessels taken from patients who underwent CABG surgery. Total RNA was reverse transcribed and cDNA was analyzed by relative quantification by RT-qPCR. JHDM1D, PHF8, HDAC1, HDAC2 and HDAC3 mRNA levels were adjusted for PBGD and ACTB levels

Oligonucleotide sequences of primers used for RT-qPCR analysis

GENEPRIMER SEQUENCE (5’–3’)
JHDM1DF TCCCTTCACCTACATTTTCTGR TGCCTGCCTCGCCACATC
PHF8F CCCTCGCCATCATTCACTGR TTCTCCCTCTTCCCGCTGT
HDAC1F GAGACGGGATTGATGACGAR TGAGCCACACTGTAAGACC
HDAC2F GAAGCCTCATAGAATCCR CTCCAACATTAAATCTCTG
HDAC3F GATGACCAGACTTACAAGR GACATATTCAACGCATTC
PBGDF GCCAAGGACCAGGACATCR TCAGGTACAGTTGCCCAT
ACTBF GCACCACACCTTCTACAATGAR ATAGCACAGCCTGGATAGC
Język:
Angielski
Częstotliwość wydawania:
4 razy w roku
Dziedziny czasopisma:
Nauki biologiczne, Biologia molekularna, Biochemia
Kanał RSS czasopisma