Otwarty dostęp

Apoptosis-related genes expression in primary in vitro culture of human ovarian granulosa cells

Ievgeniia Kocherova

Ievgeniia Kocherova

Department of Anatomy, Poznań University of Medical SciencesPoznań, Poland
Wyszukaj tego autora
Sciendo|Google Scholar
Kocherova, Ievgeniia
, 
Katarzyna Stefańska

Katarzyna Stefańska

Department of Histology and Embryology, Poznań University of Medical SciencesPoznań, Poland
Wyszukaj tego autora
Sciendo|Google Scholar
Stefańska, Katarzyna
, 
Rut Bryl

Rut Bryl

Department of Anatomy, Poznań University of Medical SciencesPoznań, Poland
Wyszukaj tego autora
Sciendo|Google Scholar
Bryl, Rut
, 
Joanna Perek

Joanna Perek

Department of Anatomy, Poznań University of Medical SciencesPoznań, Poland
Wyszukaj tego autora
Sciendo|Google Scholar
Perek, Joanna
, 
Wojciech Pieńkowski

Wojciech Pieńkowski

Division of Perinatology and Women’s Diseases, Poznań University of Medical SciencesPoznań, Poland
Wyszukaj tego autora
Sciendo|Google Scholar
Pieńkowski, Wojciech
, 
Jana Zakova

Jana Zakova

Department of Obstetrics and Gynecology, Faculty of Medicine, Masaryk University and University Hospital BrnoBrno, Czech Republic
Wyszukaj tego autora
Sciendo|Google Scholar
Zakova, Jana
, 
Igor Crha

Igor Crha

  • Department of Obstetrics and Gynecology, Faculty of Medicine, Masaryk University and University Hospital BrnoBrno, Czech Republic
  • Department of Nursing and Midwifery, Faculty of Medicine, Masaryk UniversityBrno, Czech Republic
Wyszukaj tego autora
Sciendo|Google Scholar
Crha, Igor
, 
Pavel Ventruba

Pavel Ventruba

Department of Obstetrics and Gynecology, Faculty of Medicine, Masaryk University and University Hospital BrnoBrno, Czech Republic
Wyszukaj tego autora
Sciendo|Google Scholar
Ventruba, Pavel
, 
Paul Mozdziak

Paul Mozdziak

Physiology Graduate Program, North Carolina State UniversityRaleigh, NC, USA
Wyszukaj tego autora
Sciendo|Google Scholar
Mozdziak, Paul
 oraz 
Michal Ješeta

Michal Ješeta

  • Department of Obstetrics and Gynecology, Faculty of Medicine, Masaryk University and University Hospital BrnoBrno, Czech Republic
  • Department of Veterinary Sciences, Czech University of Life Sciences in PraguePrague, Czech Republic
Wyszukaj tego autora
Sciendo|Google Scholar
Ješeta, Michal
  
31 gru 2020
Apoptosis-related genes expression in primary in vitro culture of human ovarian granulosa cells's Cover Image
O artykule
Poprzedni artykuł
Następny artykuł
Zacytuj
Pobierz okładkę
Data publikacji: 31 gru 2020
Zakres stron: 176 - 182
Otrzymano: 04 lis 2020
Przyjęty: 15 gru 2020
DOI: https://doi.org/10.2478/acb-2020-0023
Słowa kluczowe
© 2020 Ievgeniia Kocherova, Katarzyna Stefańska, Rut Bryl, Joanna Perek, Wojciech Pieńkowski, Jana Zakova, Igor Crha, Pavel Ventruba, Paul Mozdziak, Michal Ješeta, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Ovarian granulosa cells (GCs) play a crucial role in oocyte maturation, creating a favorable microenvironment around the oocyte. Therefore, enhanced apoptosis and GCs loss may negatively affect the intra-follicular milieu and compromise the oocyte quality, reducing pregnancy chances. Based on the RT-qPCR method, the present research revealed the differential expression of apoptosis-related genes (BCL2, BAX, p53, CASP9) during the seven days of primary in vitro culture of GCs isolated from patients undergoing in vitro fertilization (IVF) procedure. Individual gene expression changes may reflect the GCs survival and/or apoptotic status at different time points.

Running title: Apoptosis-related genes expression in granulosa cells in vitro

Język:
Angielski
Częstotliwość wydawania:
4 razy w roku
Dziedziny czasopisma:
Nauki biologiczne, Biologia molekularna, Biochemia
Kanał RSS czasopisma