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Apoptosis-related genes expression in primary in vitro culture of human ovarian granulosa cells

Ievgeniia Kocherova

Ievgeniia Kocherova

Department of Anatomy, Poznań University of Medical SciencesPoznań, Poland
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Kocherova, Ievgeniia
, 
Katarzyna Stefańska

Katarzyna Stefańska

Department of Histology and Embryology, Poznań University of Medical SciencesPoznań, Poland
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Stefańska, Katarzyna
, 
Rut Bryl

Rut Bryl

Department of Anatomy, Poznań University of Medical SciencesPoznań, Poland
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Bryl, Rut
, 
Joanna Perek

Joanna Perek

Department of Anatomy, Poznań University of Medical SciencesPoznań, Poland
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Perek, Joanna
, 
Wojciech Pieńkowski

Wojciech Pieńkowski

Division of Perinatology and Women’s Diseases, Poznań University of Medical SciencesPoznań, Poland
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Pieńkowski, Wojciech
, 
Jana Zakova

Jana Zakova

Department of Obstetrics and Gynecology, Faculty of Medicine, Masaryk University and University Hospital BrnoBrno, Czech Republic
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Zakova, Jana
, 
Igor Crha

Igor Crha

  • Department of Obstetrics and Gynecology, Faculty of Medicine, Masaryk University and University Hospital BrnoBrno, Czech Republic
  • Department of Nursing and Midwifery, Faculty of Medicine, Masaryk UniversityBrno, Czech Republic
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Crha, Igor
, 
Pavel Ventruba

Pavel Ventruba

Department of Obstetrics and Gynecology, Faculty of Medicine, Masaryk University and University Hospital BrnoBrno, Czech Republic
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Ventruba, Pavel
, 
Paul Mozdziak

Paul Mozdziak

Physiology Graduate Program, North Carolina State UniversityRaleigh, NC, USA
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Mozdziak, Paul
 and 
Michal Ješeta

Michal Ješeta

  • Department of Obstetrics and Gynecology, Faculty of Medicine, Masaryk University and University Hospital BrnoBrno, Czech Republic
  • Department of Veterinary Sciences, Czech University of Life Sciences in PraguePrague, Czech Republic
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Ješeta, Michal
  
Dec 31, 2020
Apoptosis-related genes expression in primary in vitro culture of human ovarian granulosa cells's Cover Image
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Published Online: Dec 31, 2020
Page range: 176 - 182
Received: Nov 04, 2020
Accepted: Dec 15, 2020
DOI: https://doi.org/10.2478/acb-2020-0023
Keywords
© 2020 Ievgeniia Kocherova, Katarzyna Stefańska, Rut Bryl, Joanna Perek, Wojciech Pieńkowski, Jana Zakova, Igor Crha, Pavel Ventruba, Paul Mozdziak, Michal Ješeta, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Ovarian granulosa cells (GCs) play a crucial role in oocyte maturation, creating a favorable microenvironment around the oocyte. Therefore, enhanced apoptosis and GCs loss may negatively affect the intra-follicular milieu and compromise the oocyte quality, reducing pregnancy chances. Based on the RT-qPCR method, the present research revealed the differential expression of apoptosis-related genes (BCL2, BAX, p53, CASP9) during the seven days of primary in vitro culture of GCs isolated from patients undergoing in vitro fertilization (IVF) procedure. Individual gene expression changes may reflect the GCs survival and/or apoptotic status at different time points.

Running title: Apoptosis-related genes expression in granulosa cells in vitro

Language:
English
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Journal Subjects:
Life Sciences, Molecular Biology, Biochemistry
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