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Analysis of TGFB1, CD105 and FSP1 expression in human granulosa cells during a 7-day primary in vitro culture

Artur Bryja

Artur Bryja

Department of Anatomy, Poznań University of Medical SciencePoznań, Poland
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Bryja, Artur
, 
Wojciech Pieńkowski

Wojciech Pieńkowski

Division of Perinatology and Women’s Diseases, Poznań University of Medical SciencesPoznań, Poland
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Pieńkowski, Wojciech
, 
Katarzyna Stefańska

Katarzyna Stefańska

Department of Histology and Embryology, Poznań University of Medical SciencePoznań, Poland
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Stefańska, Katarzyna
, 
Błażej Chermuła

Błażej Chermuła

Division of Infertility and Reproductive Endocrinology, Department of Gynecology, Obstetrics and Gynecological Oncology, Poznań University of Medical SciencesPoznań, Poland
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Chermuła, Błażej
, 
Rut Bryl

Rut Bryl

Department of Anatomy, Poznań University of Medical SciencePoznań, Poland
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Bryl, Rut
, 
Maria Wieczorkiewicz

Maria Wieczorkiewicz

Department of Basic and Preclinical Sciences, Institute of Veterinary Medicine, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in ToruńToruń, Poland
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Wieczorkiewicz, Maria
, 
Jakub Kulus

Jakub Kulus

Department of Diagnostics and Clinical Sciences, Institute of Veterinary Medicine, Nicolaus Copernicus University in ToruńToruń, Poland
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Kulus, Jakub
, 
Grzegorz Wąsiatycz

Grzegorz Wąsiatycz

Department of Veterinary Surgery, Institute of Veterinary Medicine, Nicolaus Copernicus University in ToruńToruń, Poland
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Wąsiatycz, Grzegorz
, 
Dorota Bukowska

Dorota Bukowska

Department of Diagnostics and Clinical Sciences, Institute of Veterinary Medicine, Nicolaus Copernicus University in ToruńToruń, Poland
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Bukowska, Dorota
, 
Kornel Ratajczak

Kornel Ratajczak

Department of Veterinary Surgery, Institute of Veterinary Medicine, Nicolaus Copernicus University in ToruńToruń, Poland
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Ratajczak, Kornel
, 
Jędrzej M. Jaśkowski

Jędrzej M. Jaśkowski

Department of Diagnostics and Clinical Sciences, Institute of Veterinary Medicine, Nicolaus Copernicus University in ToruńToruń, Poland
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Jaśkowski, Jędrzej M.
, 
James N. Petitte

James N. Petitte

Prestage Department of Poultry Science, North Carolina State UniversityRaleigh, NC, USA
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Petitte, James N.
, 
Paul Mozdziak

Paul Mozdziak

  • Prestage Department of Poultry Science, North Carolina State UniversityRaleigh, NC, USA
  • Physiology Graduate Program, North Carolina State UniversityRaleigh, NC, USA
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Mozdziak, Paul
, 
Leszek Pawelczyk

Leszek Pawelczyk

Division of Infertility and Reproductive Endocrinology, Department of Gynecology, Obstetrics and Gynecological Oncology, Poznań University of Medical SciencesPoznań, Poland
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Pawelczyk, Leszek
, 
Robert Z. Spaczyński

Robert Z. Spaczyński

Division of Infertility and Reproductive Endocrinology, Department of Gynecology, Obstetrics and Gynecological Oncology, Poznań University of Medical SciencesPoznań, Poland
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Spaczyński, Robert Z.
 oraz 
Paweł Antosik

Paweł Antosik

Department of Diagnostics and Clinical Sciences, Institute of Veterinary Medicine, Nicolaus Copernicus University in ToruńToruń, Poland
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Antosik, Paweł
  
31 gru 2020
Analysis of TGFB1, CD105 and FSP1 expression in human granulosa cells during a 7-day primary in vitro culture's Cover Image
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Data publikacji: 31 gru 2020
Zakres stron: 152 - 157
Otrzymano: 06 paź 2020
Przyjęty: 17 lis 2020
DOI: https://doi.org/10.2478/acb-2020-0019
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© 2020 Artur Bryja, Wojciech Pieńkowski, Katarzyna Stefańska, Błażej Chermuła, Rut Bryl, Maria Wieczorkiewicz, Jakub Kulus, Grzegorz Wąsiatycz, Dorota Bukowska, Kornel Ratajczak, Jędrzej M. Jaśkowski, James N. Petitte, Paul Mozdziak, Leszek Pawelczyk, Robert Z. Spaczyński, Paweł Antosik, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The human granulosa cells (GCs) surround the oocyte and form the ovarian follicle’s proper architecture.

These sub-populations include mural granulosa cells, antral granulosa cells, and cumulus granulosa cells.

Their main functions are to support the oocyte’s growth (cumulus granulosa cells) and estradiol production (mural granulosa cells). After ovulation, the granulosa cells transform into the luteal cells of the corpus luteum and produce progesterone.

Our study investigated the expression profile of three genes: TGFB1, CD105, and FSP1 during a 7-day in vitro culture. The analysis was conducted using the RT-qPCR technique.

Changes in the expression of CD105 and FSP1 could be observed during the 7-day in vitro culture. In the case of TGFB, the expression remained at a similar level, with no statistically significant differences observed.

Running title: Expression of TGFB1, CD105 and FSP1 in granulosa cells

Język:
Angielski
Częstotliwość wydawania:
4 razy w roku
Dziedziny czasopisma:
Nauki biologiczne, Biologia molekularna, Biochemia
Kanał RSS czasopisma