Otwarty dostęp

New Insight into Genotypic and Phenotypic Relatedness of Staphylococcus aureus Strains from Human Infections or Animal Reservoirs

KLAUDIA LISOWSKA-ŁYSIAK

KLAUDIA LISOWSKA-ŁYSIAK

Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian UniversityKrakow, Poland
Wyszukaj tego autora
Sciendo|Google Scholar
LISOWSKA-ŁYSIAK, KLAUDIA
, 
MAJA KOSECKA-STROJEK

MAJA KOSECKA-STROJEK

Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian UniversityKrakow, Poland
Wyszukaj tego autora
Sciendo|Google Scholar
KOSECKA-STROJEK, MAJA
, 
JOANNA BIAŁECKA

JOANNA BIAŁECKA

Centre of Microbiological Research and AutovaccinesKrakow, Poland
Wyszukaj tego autora
Sciendo|Google Scholar
BIAŁECKA, JOANNA
, 
ANDRZEJ KASPROWICZ

ANDRZEJ KASPROWICZ

Centre of Microbiological Research and AutovaccinesKrakow, Poland
Wyszukaj tego autora
Sciendo|Google Scholar
KASPROWICZ, ANDRZEJ
, 
KATARZYNA GARBACZ

KATARZYNA GARBACZ

Department of Oral Microbiology, Medical Faculty, Medical University of GdanskGdansk, Poland
Wyszukaj tego autora
Sciendo|Google Scholar
GARBACZ, KATARZYNA
, 
LIDIA PIECHOWICZ

LIDIA PIECHOWICZ

Department of Medical Microbiology, Medical Faculty, Medical University of GdanskGdansk, Poland
Wyszukaj tego autora
Sciendo|Google Scholar
PIECHOWICZ, LIDIA
, 
VLADIMIR KMET

VLADIMIR KMET

Centre of Biosciences, Institute of Animal Physiology, Slovak Academy of SciencesBratislava, Slovakia
Wyszukaj tego autora
Sciendo|Google Scholar
KMET, VLADIMIR
, 
VINCENZO SAVINI

VINCENZO SAVINI

Clinical Microbiology and Virology, Spirito Santo HospitalPescara (PE), Italy
Wyszukaj tego autora
Sciendo|Google Scholar
SAVINI, VINCENZO
 oraz 
JACEK MIĘDZOBRODZKI

JACEK MIĘDZOBRODZKI

Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian UniversityKrakow, Poland
Wyszukaj tego autora
Sciendo|Google Scholar
MIĘDZOBRODZKI, JACEK
  
28 mar 2019
New Insight into Genotypic and Phenotypic Relatedness of Staphylococcus aureus Strains from Human Infections or Animal Reservoirs's Cover Image
O artykule
Poprzedni artykuł
Następny artykuł
Zacytuj
Pobierz okładkę
Kategoria artykułu: original-paper
Data publikacji: 28 mar 2019
Zakres stron: 93 - 104
DOI: https://doi.org/10.21307/pjm-2019-011
Słowa kluczowe
© 2019 KLAUDIA LISOWSKA-ŁYSIAK et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Positive results of phenotypic tests.Columns A: human strains, B: animal strains. 1.1. Total number of positive results for hemolysins activity. Black: double-zone β-hemolysis; white: single-zone β-hemolysis. 1.2. Total number of positive results for proteases, nuclease, lipase and urease activity. Black, low activity. Dark grey, moderate activity. Grey, high activity. White, very high activity.
Positive results of phenotypic tests.Columns A: human strains, B: animal strains. 1.1. Total number of positive results for hemolysins activity. Black: double-zone β-hemolysis; white: single-zone β-hemolysis. 1.2. Total number of positive results for proteases, nuclease, lipase and urease activity. Black, low activity. Dark grey, moderate activity. Grey, high activity. White, very high activity.

Fig. 2.

The results of BURP analysis. The 20 spa types were assigned to proper clonal complexes. Eighteen singletons were found among the 53 strains analyzed. Black circles represent the spa types assigned to the proper clonal complexes, and the blue circles represent the ancestor within the clonal complex.
The results of BURP analysis. The 20 spa types were assigned to proper clonal complexes. Eighteen singletons were found among the 53 strains analyzed. Black circles represent the spa types assigned to the proper clonal complexes, and the blue circles represent the ancestor within the clonal complex.

Antibiotic susceptibility of S_ aureus strains_ MRSA strains are bolded in text_

AntibioticNumber of strains
Human (%) n = 26Animal (%) n = 27All (%) n = 53
Resistant to all antibiotics tested0 (0)0 (0)0 (0)
Multi-resistant1 (4)7 (26)8 (15)
BEN17 (65)16 (59)33 (62)
AMC2 (8)7 (26)9 (17)
FOX2 (8)7 (26)9 (17)
SXT0 (0)0 (0)0 (0)
CHL1 (4)1 (4)2 (4)
DOX3 (12)1 (4)4 (8)
ERY6 (23)8 (30)14 (26)
CLI0 (0)7 (26)7 (13)
AMI2 (8)2 (7)4 (8)
GEN2 (8)0 (0)2 (4)
CIP0 (0)4 (15)4 (8)
NOR0 (0)4 (15)4 (8)
Susceptible to all antibiotics tested5 (19)10 (37)15 (28)

A list of the strains collected, their origin and place of isolation_

Strain no.HostLesions/MaterialPlace of origin
1humanwound infectionKraków (Poland)
2humancystKraków (Poland)
3humanwound infectionKraków (Poland)
4humanwound infectionKraków (Poland)
5humanwound infectionKraków (Poland)
6humanwound infectionGdansk (Poland)
7humanwound infectionGdansk (Poland)
8humanboilGdansk (Poland)
9humanboilGdansk (Poland)
10humanboilGdansk (Poland)
11humanthroatGdansk (Poland)
12humanpusGdansk (Poland)
13humanthroatGdansk (Poland)
14humanthroatPescara (Italy)
15humanskin infectionPescara (Italy)
16humanbloodPescara (Italy)
17humanskin infectionPescara (Italy)
18humannose swabGdansk (Poland)
19humanulcerGdansk (Poland)
20humanulcerGdansk (Poland)
21humanwound infectionKraków (Poland)
22humanwound infectionKraków (Poland)
23humanconjunctivitisKraków (Poland)
24humanconjunctivitisKraków (Poland)
25humanwound infectionKraków (Poland)
26humannose swabKraków (Poland)
27animaleczemaGdansk (Poland)
28animaleyeGdansk (Poland)
29animaleyeGdansk (Poland)
30animalthroatGdansk (Poland)
31animalskin infectionWroclaw (Poland)
32animalmastitisKošice (Slovakia)
33animalmastitisKošice (Slovakia)
34animalmastitisKošice (Slovakia)
35animalmastitisKošice (Slovakia)
36animalmastitisKošice (Slovakia)
37animalmastitisKošice (Slovakia)
38animalmastitisKošice (Slovakia)
39animalmastitisLeczna (Poland)
40animalmastitisLeczna (Poland)
41animalmastitisLubartów (Poland)
42animalmastitisLeczna (Poland)
43animalmastitisLeczna (Poland)
44animalmastitisLuków (Poland)
45animalmastitisLuków (Poland)
46animalmastitisGawrolin (Poland)
47animalmastitisSwidnik (Poland)
48animalmastitisSwidnik (Poland)
49animalmastitisSwidnik (Poland)
50animalmastitisTomaszów Lubelski (Poland)
51animalmastitisTomaszów Lubelski (Poland)
52animalmastitisTomaszów Lubelski (Poland)
53animalmastitisTomaszów Lubelski (Poland)

The results of the spa typing_ Bacterial isolates were assigned to spa types and clonal complexes (CCs)_

Strain numberspa typesSequence type (ST)*Clonal complexResistance and virulence genes
26t008ST-8, ST-427, ST-250, ST-254spa-CC 008.051Human strains: 26/23Resistance: blaZ/I/R (+/-), tetEfflux, fosBEnterotoxins: sea(-/+), sed(+/-), seg(+/-), sej(+/-), ser(+/-)Enzymes within hemolysins: sak, hla, hlb, lukM/luk F(+/-), hlgA, lukD, lukE, lukX,Proteases: aur, splA, splB, splEAdhesion and biofilm formation: bbp, fnbB, map, sdrC, sdrD, sasG
23t051ST-250, ST-254
4t015ST-45spa-CC 015.031Human strains: 4/21Resistance: mecA(+/-), blaZ/I/R, erm(A)(-/+), tetEffluxEnterotoxins: seb(-/+), sec, seg, sej(+/-), sel(-/+), sei, sem, sen, seo, seu, egc-clusterEnzymes within hemolysins: sak, hla, hlgA, lukX,Proteases: aurAdhesion and biofilm formation: bbp, cna, fnbB, map(+/-), sdrC, sdrD
21t031ST-45
25t017ndspa-CC 017Human strains: 25/5/ animal strain: 31Resistance: blaZ/I/R(-/+/+), erm(A) (-/+/-), tet(K)(-/-/+), tetEfflux, fosBEnterotoxins: seg, sei, sem, sen, seo, seu, egc-clusterEnzymes within hemolysins: sak(+/+/-), hla, hlb, hlgA, lukX(+/-/-), lukX(-/-/+)Proteases: aur, splEAdhesion and biofilm formation: bbp, cna, fnbB(+/+/-), map(-/+/+), sdrC, sdrD
5t021ST-30, ST33, ST-55
16, 31t018ST-30, ST36, ST-38
15t026ST-45, ST-47spa-CC 026.2642Human strains: 15/2Resistance: mecA(-/+), erm(C)(-/+), addD(-/+), mupR(-/+), tetEfflux, fosB(+/-), cat(-/+)Enterotoxins: sec, seg, sei, sel(+/-), sem, sen, seo, seu, egc-clusterEnzymes within hemolysins: sak, hla, hlgA, lukX(-/+)Proteases: aurAdhesion and biofilm formation: bbp(-/+), cna(+/-), fnbB, map, sdrC, sdrD(-/+)
2t2642nd
50, 51, 52, 53t14395ndspa-CC 14395.14396Animal strains: 50/51/52/53Resistance: tetEfflux, fosBEnterotoxins: seg, sei, sem, sen, seo, seu, egc-clusterEnzymes within hemolysins: hla, hlb, hlgA, lukXProteases: aurAdhesion and biofilm formation: bbp, fnbB, map, sdrC, sdrD(51, 53: +, 50, 52: -)
32, 34t002ST-5, ST-231spa-CC 045Animal strains: 32/34/33/35Resistance: mecA,blaZ/I/R(strain no. 34: -), erm(A)(strains no. 34, 35: +), addD, tetEfflux, cat(strain no. 35: +), fosBEnterotoxins: sea(strains no. 33, 35: +), sed(strain no. 34: -), seg, sei, sej(strain no. 34: -), sem, sen, seo, ser(strain no. 34: -), seu, egc-clusterEnzymes within hemolysins: sak, hla, hlb, hlgA, lukD, lukE, lukXProteases: aur, splA, splBAdhesion and biofilm formation: bbp, fnbB(strains no. 33, 35: +), map, sdrC, sdrD, sasG
33t003ST-5, ST-225
33t003ST-5, ST-225
47, 48, 49t010ST-5Animal strains: 47/48/49Resistance: blaZ/I/R, tetEfflux, fosBEnterotoxins: seg, sei, sem, sen, seo, seu, egc-clusterEnzymes within hemolysins: hla, hlb, hlgA, lukD, lukE, lukXProteases: aur, splA, splBAdhesion and biofilm formation: bbp, map, sdrC, sdrD, sasG
1, 7, 10, 11, 12, 18, 19t084ST-15, ST-18spa-CC084Human strains: 1/7/10/11/12/18/19/8/20Animal strain: 28Resistance: blaZ/I/R(strains no. 12, 18: -), tet(K)(strains no. 7, 12, 19, 20: +), tetEfflux, fosB(strain no. 7: -)Enterotoxins: -Enzymes within hemolysins: sak(strain no. 28: +), hla(strains no. 10, 28: -), lukM/lukF (strains no. 19, 20: +), hlgA(strains no. 10, 28: -), lukD(strain no. 10: -), lukE(strains no. 18, 19: +), lukF(strain no. 20: +), lukX(strains no. 18, 19, 20: +)Proteases: aur(strains no. 10, 11, 28: -), splA, splB, splE(strains no. 10, 28: -)Adhesion and biofilm formation: bbp(strains no. 10, 8: -), fnbB(strain no. 11: -), map, sdrC, sdrD, sasG
8,9t9490nd
28t144nd
20t491nd
38t2393ndspa-CC 2393.5163Animal strains: 38/36/37Resistance: mecA, blaZ/I/R, erm(C), tet(K), tetEffluxEnterotoxins: seaEnzymes within Hemolysins: sak, hla, hlb, hlgA, lukD, lukE, lukXProteases: aur, splA, splBAdhesion and biofilm formation: bbp, fnbB, map, sdrC, sdrD, sasG
Język:
Angielski
Częstotliwość wydawania:
4 razy w roku
Dziedziny czasopisma:
Nauki biologiczne, Mikrobiologia i wirusologia
Kanał RSS czasopisma