Acceso abierto

New Insight into Genotypic and Phenotypic Relatedness of Staphylococcus aureus Strains from Human Infections or Animal Reservoirs

KLAUDIA LISOWSKA-ŁYSIAK
KLAUDIA LISOWSKA-ŁYSIAK
, 
MAJA KOSECKA-STROJEK
MAJA KOSECKA-STROJEK
, 
JOANNA BIAŁECKA
JOANNA BIAŁECKA
, 
ANDRZEJ KASPROWICZ
ANDRZEJ KASPROWICZ
, 
KATARZYNA GARBACZ
KATARZYNA GARBACZ
, 
LIDIA PIECHOWICZ
LIDIA PIECHOWICZ
, 
VLADIMIR KMET
VLADIMIR KMET
, 
VINCENZO SAVINI
VINCENZO SAVINI
 y 
JACEK MIĘDZOBRODZKI
JACEK MIĘDZOBRODZKI
   | 28 mar 2019
Polish Journal of Microbiology's Cover Image
Acerca de este artículo
Artículo anterior
Artículo siguiente
Cite
Article Category: original-paper
Publicado en línea: 28 mar 2019
Páginas: 93 - 104
DOI: https://doi.org/10.21307/pjm-2019-011
Palabras clave
© 2019 KLAUDIA LISOWSKA-ŁYSIAK et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Positive results of phenotypic tests.Columns A: human strains, B: animal strains. 1.1. Total number of positive results for hemolysins activity. Black: double-zone β-hemolysis; white: single-zone β-hemolysis. 1.2. Total number of positive results for proteases, nuclease, lipase and urease activity. Black, low activity. Dark grey, moderate activity. Grey, high activity. White, very high activity.
Positive results of phenotypic tests.Columns A: human strains, B: animal strains. 1.1. Total number of positive results for hemolysins activity. Black: double-zone β-hemolysis; white: single-zone β-hemolysis. 1.2. Total number of positive results for proteases, nuclease, lipase and urease activity. Black, low activity. Dark grey, moderate activity. Grey, high activity. White, very high activity.

Fig. 2.

The results of BURP analysis. The 20 spa types were assigned to proper clonal complexes. Eighteen singletons were found among the 53 strains analyzed. Black circles represent the spa types assigned to the proper clonal complexes, and the blue circles represent the ancestor within the clonal complex.
The results of BURP analysis. The 20 spa types were assigned to proper clonal complexes. Eighteen singletons were found among the 53 strains analyzed. Black circles represent the spa types assigned to the proper clonal complexes, and the blue circles represent the ancestor within the clonal complex.

Antibiotic susceptibility of S. aureus strains. MRSA strains are bolded in text.

AntibioticNumber of strains
Human (%) n = 26Animal (%) n = 27All (%) n = 53
Resistant to all antibiotics tested0 (0)0 (0)0 (0)
Multi-resistant1 (4)7 (26)8 (15)
BEN17 (65)16 (59)33 (62)
AMC2 (8)7 (26)9 (17)
FOX2 (8)7 (26)9 (17)
SXT0 (0)0 (0)0 (0)
CHL1 (4)1 (4)2 (4)
DOX3 (12)1 (4)4 (8)
ERY6 (23)8 (30)14 (26)
CLI0 (0)7 (26)7 (13)
AMI2 (8)2 (7)4 (8)
GEN2 (8)0 (0)2 (4)
CIP0 (0)4 (15)4 (8)
NOR0 (0)4 (15)4 (8)
Susceptible to all antibiotics tested5 (19)10 (37)15 (28)

A list of the strains collected, their origin and place of isolation.

Strain no.HostLesions/MaterialPlace of origin
1humanwound infectionKraków (Poland)
2humancystKraków (Poland)
3humanwound infectionKraków (Poland)
4humanwound infectionKraków (Poland)
5humanwound infectionKraków (Poland)
6humanwound infectionGdansk (Poland)
7humanwound infectionGdansk (Poland)
8humanboilGdansk (Poland)
9humanboilGdansk (Poland)
10humanboilGdansk (Poland)
11humanthroatGdansk (Poland)
12humanpusGdansk (Poland)
13humanthroatGdansk (Poland)
14humanthroatPescara (Italy)
15humanskin infectionPescara (Italy)
16humanbloodPescara (Italy)
17humanskin infectionPescara (Italy)
18humannose swabGdansk (Poland)
19humanulcerGdansk (Poland)
20humanulcerGdansk (Poland)
21humanwound infectionKraków (Poland)
22humanwound infectionKraków (Poland)
23humanconjunctivitisKraków (Poland)
24humanconjunctivitisKraków (Poland)
25humanwound infectionKraków (Poland)
26humannose swabKraków (Poland)
27animaleczemaGdansk (Poland)
28animaleyeGdansk (Poland)
29animaleyeGdansk (Poland)
30animalthroatGdansk (Poland)
31animalskin infectionWroclaw (Poland)
32animalmastitisKošice (Slovakia)
33animalmastitisKošice (Slovakia)
34animalmastitisKošice (Slovakia)
35animalmastitisKošice (Slovakia)
36animalmastitisKošice (Slovakia)
37animalmastitisKošice (Slovakia)
38animalmastitisKošice (Slovakia)
39animalmastitisLeczna (Poland)
40animalmastitisLeczna (Poland)
41animalmastitisLubartów (Poland)
42animalmastitisLeczna (Poland)
43animalmastitisLeczna (Poland)
44animalmastitisLuków (Poland)
45animalmastitisLuków (Poland)
46animalmastitisGawrolin (Poland)
47animalmastitisSwidnik (Poland)
48animalmastitisSwidnik (Poland)
49animalmastitisSwidnik (Poland)
50animalmastitisTomaszów Lubelski (Poland)
51animalmastitisTomaszów Lubelski (Poland)
52animalmastitisTomaszów Lubelski (Poland)
53animalmastitisTomaszów Lubelski (Poland)

The results of the spa typing. Bacterial isolates were assigned to spa types and clonal complexes (CCs).

Strain numberspa typesSequence type (ST)*Clonal complexResistance and virulence genes
26t008ST-8, ST-427, ST-250, ST-254spa-CC 008.051Human strains: 26/23Resistance: blaZ/I/R (+/-), tetEfflux, fosBEnterotoxins: sea(-/+), sed(+/-), seg(+/-), sej(+/-), ser(+/-)Enzymes within hemolysins: sak, hla, hlb, lukM/luk F(+/-), hlgA, lukD, lukE, lukX,Proteases: aur, splA, splB, splEAdhesion and biofilm formation: bbp, fnbB, map, sdrC, sdrD, sasG
23t051ST-250, ST-254
4t015ST-45spa-CC 015.031Human strains: 4/21Resistance: mecA(+/-), blaZ/I/R, erm(A)(-/+), tetEffluxEnterotoxins: seb(-/+), sec, seg, sej(+/-), sel(-/+), sei, sem, sen, seo, seu, egc-clusterEnzymes within hemolysins: sak, hla, hlgA, lukX,Proteases: aurAdhesion and biofilm formation: bbp, cna, fnbB, map(+/-), sdrC, sdrD
21t031ST-45
25t017ndspa-CC 017Human strains: 25/5/ animal strain: 31Resistance: blaZ/I/R(-/+/+), erm(A) (-/+/-), tet(K)(-/-/+), tetEfflux, fosBEnterotoxins: seg, sei, sem, sen, seo, seu, egc-clusterEnzymes within hemolysins: sak(+/+/-), hla, hlb, hlgA, lukX(+/-/-), lukX(-/-/+)Proteases: aur, splEAdhesion and biofilm formation: bbp, cna, fnbB(+/+/-), map(-/+/+), sdrC, sdrD
5t021ST-30, ST33, ST-55
16, 31t018ST-30, ST36, ST-38
15t026ST-45, ST-47spa-CC 026.2642Human strains: 15/2Resistance: mecA(-/+), erm(C)(-/+), addD(-/+), mupR(-/+), tetEfflux, fosB(+/-), cat(-/+)Enterotoxins: sec, seg, sei, sel(+/-), sem, sen, seo, seu, egc-clusterEnzymes within hemolysins: sak, hla, hlgA, lukX(-/+)Proteases: aurAdhesion and biofilm formation: bbp(-/+), cna(+/-), fnbB, map, sdrC, sdrD(-/+)
2t2642nd
50, 51, 52, 53t14395ndspa-CC 14395.14396Animal strains: 50/51/52/53Resistance: tetEfflux, fosBEnterotoxins: seg, sei, sem, sen, seo, seu, egc-clusterEnzymes within hemolysins: hla, hlb, hlgA, lukXProteases: aurAdhesion and biofilm formation: bbp, fnbB, map, sdrC, sdrD(51, 53: +, 50, 52: -)
32, 34t002ST-5, ST-231spa-CC 045Animal strains: 32/34/33/35Resistance: mecA,blaZ/I/R(strain no. 34: -), erm(A)(strains no. 34, 35: +), addD, tetEfflux, cat(strain no. 35: +), fosBEnterotoxins: sea(strains no. 33, 35: +), sed(strain no. 34: -), seg, sei, sej(strain no. 34: -), sem, sen, seo, ser(strain no. 34: -), seu, egc-clusterEnzymes within hemolysins: sak, hla, hlb, hlgA, lukD, lukE, lukXProteases: aur, splA, splBAdhesion and biofilm formation: bbp, fnbB(strains no. 33, 35: +), map, sdrC, sdrD, sasG
33t003ST-5, ST-225
33t003ST-5, ST-225
47, 48, 49t010ST-5Animal strains: 47/48/49Resistance: blaZ/I/R, tetEfflux, fosBEnterotoxins: seg, sei, sem, sen, seo, seu, egc-clusterEnzymes within hemolysins: hla, hlb, hlgA, lukD, lukE, lukXProteases: aur, splA, splBAdhesion and biofilm formation: bbp, map, sdrC, sdrD, sasG
1, 7, 10, 11, 12, 18, 19t084ST-15, ST-18spa-CC084Human strains: 1/7/10/11/12/18/19/8/20Animal strain: 28Resistance: blaZ/I/R(strains no. 12, 18: -), tet(K)(strains no. 7, 12, 19, 20: +), tetEfflux, fosB(strain no. 7: -)Enterotoxins: -Enzymes within hemolysins: sak(strain no. 28: +), hla(strains no. 10, 28: -), lukM/lukF (strains no. 19, 20: +), hlgA(strains no. 10, 28: -), lukD(strain no. 10: -), lukE(strains no. 18, 19: +), lukF(strain no. 20: +), lukX(strains no. 18, 19, 20: +)Proteases: aur(strains no. 10, 11, 28: -), splA, splB, splE(strains no. 10, 28: -)Adhesion and biofilm formation: bbp(strains no. 10, 8: -), fnbB(strain no. 11: -), map, sdrC, sdrD, sasG
8,9t9490nd
28t144nd
20t491nd
38t2393ndspa-CC 2393.5163Animal strains: 38/36/37Resistance: mecA, blaZ/I/R, erm(C), tet(K), tetEffluxEnterotoxins: seaEnzymes within Hemolysins: sak, hla, hlb, hlgA, lukD, lukE, lukXProteases: aur, splA, splBAdhesion and biofilm formation: bbp, fnbB, map, sdrC, sdrD, sasG
eISSN:
2544-4646
Idioma:
Inglés
Calendario de la edición:
4 veces al año
Temas de la revista:
Life Sciences, Microbiology and Virology
RSS Feed de revista