Isolate | Biotype | A number of bacterial cells adhering to Hep-2 cells | A number of bacterial cells surviving within Hep-2 cells |
---|---|---|---|
2006RAT | 1A | 5 000 ± 10 | 10 000 ± 12 |
SDWL-003 | 1A | 1 000 ± 10 | 500 ± 7 |
Ye92010 | 1B | 750 000 ± 23 | 4 500 ± 10 |
NX-SA98-837 | 3 | 2 100 000 ± 36 | 32 000 ± 16 |
Strains | Biochemical tests | Biotype | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
Lipase (Tween-esterase) | Esculin/salicin 24 h | Indole | Xylose | Trehalose/NO3 | Pyrazinamidase | β-D-Glucosidase | Voges-Proskauer | Proline peptidase | ||
2006RAT | + | + | + | + | + | + | + | + | V1 | 1A |
SDWL-003 | + | + | + | + | + | + | + | + | V1 | 1A |
Ye92010 | + | – | + | + | + | – | – | + | – | 1B |
NX-SA98-837 | – | – | – | + | + | – | – | + | – | 3 |
Number of samples | Source | Ct ranges in HEX channel for positive results | Ct ranges in HEX channel for negative results |
---|---|---|---|
Positive control | Control DNA | 28.89 | – |
17 | Pig tonsils | 25.67–36.17 | – |
83 | Pig tonsils | – | 37.23–37.63 |
Negative control | PCR water | – | 37.34 |