<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The application of DNA-based methods enables to identify <italic>Yersinia enterocolitica</italic> carrying the <italic>ail</italic>-gene with a greater sensitivity compared to culture methods and biochemical tests used for detection of pathogenic <italic>Y. enterocolitica</italic> in animal and food samples. In this study, 100 samples of pig tonsils were examined, among which 17 were positive for the <italic>ail</italic> gene. Additionally, biochemical tests and RT-PCR showed that nine <italic>Y. enterocolitica</italic> isolates carried the <italic>ail</italic>-gene. Two <italic>Y. enterocolitica</italic> isolates of 1A biotype had the <italic>ail</italic> gene. The results demonstrated the usefulness of RT-PCR method applied for detection of potentially pathogenic, possessing the <italic>ail</italic> gene <italic>Y. enterocolitica</italic> in the material examined.</p></abstract>

The application of DNA-based methods enables to identify Yersinia enterocolitica carrying the ail-gene with a greater sensitivity compared to culture methods and biochemical tests used for detection of pathogenic Y. enterocolitica in animal and food samples. In this study, 100 samples of pig tonsils were examined, among which 17 were positive for the ail gene. Additionally, biochemical tests and RT-PCR showed that nine Y. enterocolitica isolates carried the ail-gene. Two Y. enterocolitica isolates of 1A biotype had the ail gene. The results demonstrated the usefulness of RT-PCR method applied for detection of potentially pathogenic, possessing the ail gene Y. enterocolitica in the material examined.

Identification of Pathogenicity of <italic>Yersinia enterocolitica</italic> in Pig Tonsils Using the Real-Time PCR

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Identification of Pathogenicity of  in Pig Tonsils Using the Real-Time PCR

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The application of DNA-based methods enables to identify Yersinia enterocolitica carrying the ail-gene with a greater sensitivity compared to culture methods...

Isolate	Biotype	A number of bacterial cells adhering to Hep-2 cells	A number of bacterial cells surviving within Hep-2 cells
2006RAT	1A	5 000 ± 10^T	10 000 ± 12
SDWL-003	1A	1 000 ± 10	500 ± 7
Ye92010	1B	750 000 ± 23	4 500 ± 10
NX-SA98-837	3	2 100 000 ± 36	32 000 ± 16

Strains	Biochemical tests									Biotype
Strains	Lipase (Tween-esterase)	Esculin/salicin 24 h	Indole	Xylose	Trehalose/NO₃	Pyrazinamidase	β-D-Glucosidase	Voges-Proskauer	Proline peptidase	Biotype
2006RAT	+	+	+	+	+	+	+	+	V¹	1A
SDWL-003	+	+	+	+	+	+	+	+	V¹	1A
Ye92010	+	–	+	+	+	–	–	+	–	1B
NX-SA98-837	–	–	–	+	+	–	–	+	–	3

Number of samples	Source	Ct ranges in HEX channel for positive results	Ct ranges in HEX channel for negative results
Positive control	Control DNA	28.89	–
17	Pig tonsils	25.67–36.17	–
83	Pig tonsils	–	37.23–37.63
Negative control	PCR water	–	37.34

Identification of Pathogenicity of Yersinia enterocolitica in Pig Tonsils Using the Real-Time PCR

Article Category: short-communication

Online veröffentlicht: 30. Juni 2018

Seitenbereich: 219 - 222

Eingereicht: 06. Juni 2017

Akzeptiert: 22. Feb. 2018

DOI: https://doi.org/10.21307/pjm-2018-023

Schlüsselwörter
RT-PCR, pathogen specific gene (), biochemical testing, biotyping

© 2018 Milena A. Stachelska published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The adhesion and invasion properties of Yersinia enterocolitica biotype 1A containing the ail gene in comparison to biotype 3.

Assessment of biotypes of Yersinia enterocolitica isolates according to Wauters et al. scheme (1987).

Detection of pathogenic Yersinia enterocolitica in pig tonsils with RT-PCR.

Identification of Pathogenicity of Yersinia enterocolitica in Pig Tonsils Using the Real-Time PCR

Article Category: short-communication

Online veröffentlicht: 30. Juni 2018

Seitenbereich: 219 - 222

Eingereicht: 06. Juni 2017

Akzeptiert: 22. Feb. 2018

DOI: https://doi.org/10.21307/pjm-2018-023

SchlüsselwörterRT-PCR, pathogen specific gene (), biochemical testing, biotyping

© 2018 Milena A. Stachelska published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The adhesion and invasion properties of Yersinia enterocolitica biotype 1A containing the ail gene in comparison to biotype 3.

Assessment of biotypes of Yersinia enterocolitica isolates according to Wauters et al. scheme (1987).

Detection of pathogenic Yersinia enterocolitica in pig tonsils with RT-PCR.

Schlüsselwörter
RT-PCR, pathogen specific gene (), biochemical testing, biotyping