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The gut microbiota of Cystidicola farionis parasitizing the swim bladder of the nosed charr morph Salvelinus malma complex in Lake Kronotskoe (Kamchatka, Russia)

E.N. Kashinskaya

E.N. Kashinskaya

Institute of Systematics and Ecology of Animals of Siberian Branch of Russian Academy of Sciences; Research group of physiology and genetics of hydrobiontsNovosibirsk, Russia
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Kashinskaya, E.N.
, 
E.P. Simonov

E.P. Simonov

  • Institute of Systematics and Ecology of Animals of Siberian Branch of Russian Academy of Sciences; Research group of physiology and genetics of hydrobiontsNovosibirsk, Russia
  • University of Tyumen, Institute of Environmental and Agricultural Biology (X-BIO)Tyumen, Russia
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Simonov, E.P.
, 
P.G. Vlasenko

P.G. Vlasenko

Institute of Systematics and Ecology of Animals of Siberian Branch of Russian Academy of Sciences; Research group of physiology and genetics of hydrobiontsNovosibirsk, Russia
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Vlasenko, P.G.
, 
G.N. Markevich

G.N. Markevich

Kronotsky State Nature Biosphere ReserveYelizovo, Russia
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Markevich, G.N.
, 
A.V. Shokurova

A.V. Shokurova

Institute of Systematics and Ecology of Animals of Siberian Branch of Russian Academy of Sciences; Research group of physiology and genetics of hydrobiontsNovosibirsk, Russia
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Shokurova, A.V.
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K.B. Andree

K.B. Andree

Instituto de Investigación y Tecnología Agroalimentarias; Cultius Aquàtics; San Carlos de la RapitaTarragona, Spain
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Andree, K.B.
 oraz 
M.M. Solovyev

M.M. Solovyev

  • Institute of Systematics and Ecology of Animals of Siberian Branch of Russian Academy of Sciences; Research group of physiology and genetics of hydrobiontsNovosibirsk, Russia
  • Tomsk State University; Institute of Biology, Ecology, Soil Science, Agriculture, and ForestryTomsk, Russia
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Solovyev, M.M.
  
15 gru 2021
The gut microbiota of Cystidicola farionis parasitizing the swim bladder of the nosed charr morph Salvelinus malma complex in Lake Kronotskoe (Kamchatka, Russia)'s Cover Image
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Data publikacji: 15 gru 2021
Zakres stron: 1 - 15
DOI: https://doi.org/10.21307/jofnem-2021-106
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© 2021 E.N. Kashinskaya et al., published by Sciendo.
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1:

Sampling site (A) of C. farionis (C) parasitizing the swim bladder of different morphs of the nosed charr (B). Picture by P.G. Vlasenko.
Sampling site (A) of C. farionis (C) parasitizing the swim bladder of different morphs of the nosed charr (B). Picture by P.G. Vlasenko.

Figure 2:

18S rRNA gene tree reconstruction based on ML methods.
18S rRNA gene tree reconstruction based on ML methods.

Figure 3:

Diversity analysis of microbial community associated with nematodes Cystidicola farionis. (A) The richness and diversity estimates of microbial communities. (B) Principal coordinates analysis (PCoA) for nematode-associated microbiota: red—“body”, green—swim bladder, blue—gut. The asterisk character indicates significance at p ≤ 0.05.
Diversity analysis of microbial community associated with nematodes Cystidicola farionis. (A) The richness and diversity estimates of microbial communities. (B) Principal coordinates analysis (PCoA) for nematode-associated microbiota: red—“body”, green—swim bladder, blue—gut. The asterisk character indicates significance at p ≤ 0.05.

Figure 4:

Diversity analysis of the microbial community associated with nematodes Cystidicola farionis parasitizing the swim bladder of two morphs of nosed charr. (A) The richness and diversity estimates of microbial communities. (B) Principal coordinates analysis (PCoA) for nematode-associated microbiota: red—morph N1g of nosed charr, blue—morph N2 of nosed charr. The asterisk character indicates significance at p ≤ 0.05.
Diversity analysis of the microbial community associated with nematodes Cystidicola farionis parasitizing the swim bladder of two morphs of nosed charr. (A) The richness and diversity estimates of microbial communities. (B) Principal coordinates analysis (PCoA) for nematode-associated microbiota: red—morph N1g of nosed charr, blue—morph N2 of nosed charr. The asterisk character indicates significance at p ≤ 0.05.

Figure 5:

Taxonomical composition of microbial communities identified in gut, “body” remains of Cystidicola farionis and microbiota of swim bladder of nosed charr. Numbers 1 to 4 along x-axis denotes the number of individual of nosed charr per pool; N1g/N2 indicates an ecological group of fish, «whole» indicates whole nematode without dissection of their “body”. (A) Phylum composition: () Actinobacteria, () Bacteroidetes, () Chloroflexi, () Firmicutes, () Fusobacteria, () Proteobacteria, (), Others<1%. (b) Genus and family proportions: () Corynebacterium 1, () Lawsonella, () Cutibacterium, () Nodosilinea PCC-7104, () Tumebacillus, () unc. Alicyclobacillaceae, () Bacillus, () Staphylococcus, () Cetobacterium, () Caulobacter, () Paracoccus, () Pajaroellobacter, () Aeromonas, () Shewanella, () Ambiguous taxa of Enterobacteriaceae, () Yersinia, () unc. other Enterobacteriaceae, () Acinetobacter, () Pseudomonas, () unc. Gammaproteobacteria, () Other < 3%.
Taxonomical composition of microbial communities identified in gut, “body” remains of Cystidicola farionis and microbiota of swim bladder of nosed charr. Numbers 1 to 4 along x-axis denotes the number of individual of nosed charr per pool; N1g/N2 indicates an ecological group of fish, «whole» indicates whole nematode without dissection of their “body”. (A) Phylum composition: () Actinobacteria, () Bacteroidetes, () Chloroflexi, () Firmicutes, () Fusobacteria, () Proteobacteria, (), Others<1%. (b) Genus and family proportions: () Corynebacterium 1, () Lawsonella, () Cutibacterium, () Nodosilinea PCC-7104, () Tumebacillus, () unc. Alicyclobacillaceae, () Bacillus, () Staphylococcus, () Cetobacterium, () Caulobacter, () Paracoccus, () Pajaroellobacter, () Aeromonas, () Shewanella, () Ambiguous taxa of Enterobacteriaceae, () Yersinia, () unc. other Enterobacteriaceae, () Acinetobacter, () Pseudomonas, () unc. Gammaproteobacteria, () Other < 3%.

Nearest-match identification of C_ farionis to known sequences in NCBI database_

GenBank acc. no. Gene Species
JF803919.1 small subunit ribosomal RNA gene (18S) partial C. farionis
MG594291.1 small subunit ribosomal RNA gene (18S) partial C. farionis
DQ094172.1 small subunit ribosomal RNA gene (18S) partial Ascarophis arctica
MN294781.1 small subunit ribosomal RNA gene (18S) partial Comephoronema werestschagini
MN294783.1 small subunit ribosomal RNA gene (18S) partial Capillospirura ovotrichuria
MG594289.1 small subunit ribosomal RNA gene (18S) partial Capillospirura sp.
JF934733.1 small subunit ribosomal RNA gene (18S) partial Proleptus sp.
DQ442660.1 small subunit ribosomal RNA gene (18S) partial Neoascarophis macrouri
JF803921.1 small subunit ribosomal RNA gene (18S) partial N. longispicula
JF803926.1 small subunit ribosomal RNA gene (18S) partial Heliconema longissimum
JF803949.1 small subunit ribosomal RNA gene (18S) partial H. longissimum
JF803930.1 small subunit ribosomal RNA gene (18S) partial Ascarophis adioryx
MT086834.1 large subunit ribosomal RNA gene (28S) partial C. farionis

Descriptions of samples used in this study_

Ecological group of fish Sex Body weight, g Total length, mm Fork length, mm Type of sample for microbiome analysis Number of pooled samples
1-N1g F 304.4 355.0 309.0 Gut 15
Gut 15
“Body” 15
“Body” 15
Swim bladder 1
2-N1g M 652.1 438.0 400.0 Gut 20
“Body” 20
Swim bladder 1
3-N2 M 229.6 292.0 260.0 Gut 5
Gut 5
Gut 10
Gut 25
“Body” 5
“Body” 5
“Body” 10
“Body” 25
Swim bladder 1
4-N2 F 294.3 295.0 333.0 Gut 20
“Body” 20
Swim bladder 1

Prevalence, intensity and mean abundance of C_ farionis in swim bladder of three morphs of nosed charr at different sampling times_

Year Month Prevalence, % Intensity Mean abundance Number of fish
Nosed charr N1 (n = 227) a
 2013 VI-VIII 69 0–328 83.0 ± 3.9 13
 2014 VI 96 0–452 144.0 ± 15.4 49
VII 84 0–335 131.0 ± 13.6 50
VIII 87 0–526 150.0 ± 18.2 38
IX – b – – –
X 94 0–550 140.0 ± 18.2 47
XI 100 12–508 154.9 ± 23.4 30
Nosed charr N1g (n = 2)
 2020 IX-X 100 15–20 16.6 ± 5.0 2
Nosed charr N2 (n = 57)
 2013 VI-VIII 82 0–685 172.0 ± 44.8 17
 2014 VI 63 0–274 126.0 ± 42.0 8
VII 80 0–362 179.0 ± 38.9 10
VIII 100 43–252 118.0 ± 28.8 8
IX 100 23 23.0 1
X 100 130–376 243.0 ± 33.1 8
XI 100 155–466 333.0 ± 92.0 3
 2020 IX-X 100 5–45 32.5 ± 12.5 2
Nosed charr N3 (n = 37)
 2013 VI-VIII 88 0–519 205.0 ± 58.2 8
 2014 VI – – – –
VII 100 28–225 130.0 ± 44.5 4
VIII 100 420 420.0 1
IX 74 0–466 102.0 ± 24.04 23
X 100 318 318.0 1
XI – – – –
 2020 IX-X – – – –
Język:
Angielski
Częstotliwość wydawania:
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