The gut microbiota of  parasitizing the swim bladder of the nosed charr morph  complex in Lake Kronotskoe (Kamchatka, Russia)

E.N. Kashinskaya; E.P. Simonov; P.G. Vlasenko; G.N. Markevich; A.V. Shokurova; K.B. Andree; M.M. Solovyev

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The gut microbiota of Cystidicola farionis parasitizing the swim bladder of the nosed charr morph Salvelinus malma complex in Lake Kronotskoe (Kamchatka, Russia)

| 15 dic 2021

Journal of Nematology

Volume 53 (2021): Numero 1 (January 2021)

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Pubblicato online: 15 dic 2021

Pagine: 1 - 15

DOI: https://doi.org/10.21307/jofnem-2021-106

Parole chiave
Gut microbiota, Nosed charr, complex, V3–V4 region of 16S rRNA, 18S and 28S rRNA

© 2021 E.N. Kashinskaya et al., published by Sciendo.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Sampling site (A) of C. farionis (C) parasitizing the swim bladder of different morphs of the nosed charr (B). Picture by P.G. Vlasenko.

18S rRNA gene tree reconstruction based on ML methods.

Diversity analysis of microbial community associated with nematodes Cystidicola farionis. (A) The richness and diversity estimates of microbial communities. (B) Principal coordinates analysis (PCoA) for nematode-associated microbiota: red—“body”, green—swim bladder, blue—gut. The asterisk character indicates significance at p ≤ 0.05.

Diversity analysis of the microbial community associated with nematodes Cystidicola farionis parasitizing the swim bladder of two morphs of nosed charr. (A) The richness and diversity estimates of microbial communities. (B) Principal coordinates analysis (PCoA) for nematode-associated microbiota: red—morph N1g of nosed charr, blue—morph N2 of nosed charr. The asterisk character indicates significance at p ≤ 0.05.

Taxonomical composition of microbial communities identified in gut, “body” remains of Cystidicola farionis and microbiota of swim bladder of nosed charr. Numbers 1 to 4 along x-axis denotes the number of individual of nosed charr per pool; N1g/N2 indicates an ecological group of fish, «whole» indicates whole nematode without dissection of their “body”. (A) Phylum composition: () Actinobacteria, () Bacteroidetes, () Chloroflexi, () Firmicutes, () Fusobacteria, () Proteobacteria, (), Others<1%. (b) Genus and family proportions: () Corynebacterium 1, () Lawsonella, () Cutibacterium, () Nodosilinea PCC-7104, () Tumebacillus, () unc. Alicyclobacillaceae, () Bacillus, () Staphylococcus, () Cetobacterium, () Caulobacter, () Paracoccus, () Pajaroellobacter, () Aeromonas, () Shewanella, () Ambiguous taxa of Enterobacteriaceae, () Yersinia, () unc. other Enterobacteriaceae, () Acinetobacter, () Pseudomonas, () unc. Gammaproteobacteria, () Other < 3%.

Nearest-match identification of C. farionis to known sequences in NCBI database.

GenBank acc. no.	Gene	Species
JF803919.1	small subunit ribosomal RNA gene (18S) partial	C. farionis
MG594291.1	small subunit ribosomal RNA gene (18S) partial	C. farionis
DQ094172.1	small subunit ribosomal RNA gene (18S) partial	Ascarophis arctica
MN294781.1	small subunit ribosomal RNA gene (18S) partial	Comephoronema werestschagini
MN294783.1	small subunit ribosomal RNA gene (18S) partial	Capillospirura ovotrichuria
MG594289.1	small subunit ribosomal RNA gene (18S) partial	Capillospirura sp.
JF934733.1	small subunit ribosomal RNA gene (18S) partial	Proleptus sp.
DQ442660.1	small subunit ribosomal RNA gene (18S) partial	Neoascarophis macrouri
JF803921.1	small subunit ribosomal RNA gene (18S) partial	N. longispicula
JF803926.1	small subunit ribosomal RNA gene (18S) partial	Heliconema longissimum
JF803949.1	small subunit ribosomal RNA gene (18S) partial	H. longissimum
JF803930.1	small subunit ribosomal RNA gene (18S) partial	Ascarophis adioryx
MT086834.1	large subunit ribosomal RNA gene (28S) partial	C. farionis

Descriptions of samples used in this study.

Ecological group of fish	Sex	Body weight, g	Total length, mm	Fork length, mm	Type of sample for microbiome analysis	Number of pooled samples
1-N1g	F	304.4	355.0	309.0	Gut	15
					Gut	15
					“Body”	15
					“Body”	15
					Swim bladder	1
2-N1g	M	652.1	438.0	400.0	Gut	20
					“Body”	20
					Swim bladder	1
3-N2	M	229.6	292.0	260.0	Gut	5
					Gut	5
					Gut	10
					Gut	25
					“Body”	5
					“Body”	5
					“Body”	10
					“Body”	25
					Swim bladder	1
4-N2	F	294.3	295.0	333.0	Gut	20
					“Body”	20
					Swim bladder	1

Prevalence, intensity and mean abundance of C. farionis in swim bladder of three morphs of nosed charr at different sampling times.

Year	Month	Prevalence, %	Intensity	Mean abundance	Number of fish
Nosed charr N1 (n = 227) ^a
2013	VI-VIII	69	0–328	83.0 ± 3.9	13
2014	VI	96	0–452	144.0 ± 15.4	49
	VII	84	0–335	131.0 ± 13.6	50
	VIII	87	0–526	150.0 ± 18.2	38
	IX	– ^b	–	–	–
	X	94	0–550	140.0 ± 18.2	47
	XI	100	12–508	154.9 ± 23.4	30
Nosed charr N1g (n = 2)
2020	IX-X	100	15–20	16.6 ± 5.0	2
Nosed charr N2 (n = 57)
2013	VI-VIII	82	0–685	172.0 ± 44.8	17
2014	VI	63	0–274	126.0 ± 42.0	8
	VII	80	0–362	179.0 ± 38.9	10
	VIII	100	43–252	118.0 ± 28.8	8
	IX	100	23	23.0	1
	X	100	130–376	243.0 ± 33.1	8
	XI	100	155–466	333.0 ± 92.0	3
2020	IX-X	100	5–45	32.5 ± 12.5	2
Nosed charr N3 (n = 37)
2013	VI-VIII	88	0–519	205.0 ± 58.2	8
2014	VI	–	–	–	–
	VII	100	28–225	130.0 ± 44.5	4
	VIII	100	420	420.0	1
	IX	74	0–466	102.0 ± 24.04	23
	X	100	318	318.0	1
	XI	–	–	–	–
2020	IX-X	–	–	–	–

eISSN:: 2640-396X
Lingua:: Inglese

Frequenza di pubblicazione:: Volume Open
Argomenti della rivista:: Life Sciences, other

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The gut microbiota of Cystidicola farionis parasitizing the swim bladder of the nosed charr morph Salvelinus malma complex in Lake Kronotskoe (Kamchatka, Russia)

Pubblicato online: 15 dic 2021

Pagine: 1 - 15

DOI: https://doi.org/10.21307/jofnem-2021-106

Parole chiave
Gut microbiota, Nosed charr, complex, V3–V4 region of 16S rRNA, 18S and 28S rRNA

© 2021 E.N. Kashinskaya et al., published by Sciendo.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1:

Figure 2:

Figure 3:

Figure 4:

Figure 5:

Nearest-match identification of C. farionis to known sequences in NCBI database.

Descriptions of samples used in this study.

Prevalence, intensity and mean abundance of C. farionis in swim bladder of three morphs of nosed charr at different sampling times.

The gut microbiota of Cystidicola farionis parasitizing the swim bladder of the nosed charr morph Salvelinus malma complex in Lake Kronotskoe (Kamchatka, Russia)

Pubblicato online: 15 dic 2021

Pagine: 1 - 15

DOI: https://doi.org/10.21307/jofnem-2021-106

Parole chiaveGut microbiota, Nosed charr, complex, V3–V4 region of 16S rRNA, 18S and 28S rRNA

© 2021 E.N. Kashinskaya et al., published by Sciendo.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1:

Figure 2:

Figure 3:

Figure 4:

Figure 5:

Nearest-match identification of C. farionis to known sequences in NCBI database.

Descriptions of samples used in this study.

Prevalence, intensity and mean abundance of C. farionis in swim bladder of three morphs of nosed charr at different sampling times.

Parole chiave
Gut microbiota, Nosed charr, complex, V3–V4 region of 16S rRNA, 18S and 28S rRNA