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Morphological and molecular characterisation of Longidorus pauli (Nematoda: Longidoridae), first report from Greece

Emmanuel A. Tzortzakakis
Emmanuel A. Tzortzakakis
, 
Ilenia Clavero-Camacho
Ilenia Clavero-Camacho
, 
Carolina Cantalapiedra-Navarrete
Carolina Cantalapiedra-Navarrete
, 
Parthenopi Ralli
Parthenopi Ralli
, 
Juan E. Palomares-Rius
Juan E. Palomares-Rius
, 
Pablo Castillo
Pablo Castillo
 oraz 
Antonio Archidona-Yuste
Antonio Archidona-Yuste
   | 01 sty 2021
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Data publikacji: 01 sty 2021
Zakres stron: 1 - 10
DOI: https://doi.org/10.21307/jofnem-2021-034
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© 2021 Authors, published by Sciendo.
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1:

Light micrographs of Longidorus pauli (Lamberti et al., 1999) from Greece (A-N). A-E: Female anterior regions. F: Detail of base of odontostyle and odontophore, G: Vulval region showing sperm cells in the uterus, H-K: Female tail regions, L: Detail of sperm cells in male genital tract, M: Male tail region, N: Detail of spicules. a = anus; af = amphidial fovea; as = adanal supplement; c = cloaca; gr = guiding ring; Odt = odontostyle; Odp = odontophore; sp = spicules; spc = sperm cells; V = vulva. (Scale bars: A-N = 20 µm).
Light micrographs of Longidorus pauli (Lamberti et al., 1999) from Greece (A-N). A-E: Female anterior regions. F: Detail of base of odontostyle and odontophore, G: Vulval region showing sperm cells in the uterus, H-K: Female tail regions, L: Detail of sperm cells in male genital tract, M: Male tail region, N: Detail of spicules. a = anus; af = amphidial fovea; as = adanal supplement; c = cloaca; gr = guiding ring; Odt = odontostyle; Odp = odontophore; sp = spicules; spc = sperm cells; V = vulva. (Scale bars: A-N = 20 µm).

Figure 2:

Phylogenetic relationships of Longidorus pauli (Lamberti et al., 1999) within the genus Longidorus. Bayesian 50% majority rule consensus tree as inferred from D2 and D3 expansion domains of 28S rRNA sequence alignment under the general time-reversible model of sequence evolution with correction for invariable sites and a gamma-shaped distribution (GTR + I + G). Posterior probabilities more than 0.70 are given for appropriate clades. Newly obtained sequences in this study are shown in bold. Scale bar = expected changes per site.
Phylogenetic relationships of Longidorus pauli (Lamberti et al., 1999) within the genus Longidorus. Bayesian 50% majority rule consensus tree as inferred from D2 and D3 expansion domains of 28S rRNA sequence alignment under the general time-reversible model of sequence evolution with correction for invariable sites and a gamma-shaped distribution (GTR + I + G). Posterior probabilities more than 0.70 are given for appropriate clades. Newly obtained sequences in this study are shown in bold. Scale bar = expected changes per site.

Figure 3:

Phylogenetic relationships of Longidorus pauli (Lamberti et al., 1999) from Greece within the genus Longidorus. Bayesian 50% majority rule consensus tree as inferred from ITS1 rRNA sequence alignment under the TIM3 + G model. Posterior probabilities more than 0.70 are given for appropriate clades. Newly obtained sequences in this study are shown in bold. Scale bar = expected changes per site.
Phylogenetic relationships of Longidorus pauli (Lamberti et al., 1999) from Greece within the genus Longidorus. Bayesian 50% majority rule consensus tree as inferred from ITS1 rRNA sequence alignment under the TIM3 + G model. Posterior probabilities more than 0.70 are given for appropriate clades. Newly obtained sequences in this study are shown in bold. Scale bar = expected changes per site.

Figure 4:

Phylogenetic relationships of Longidorus pauli (Lamberti et al., 1999) within the genus Longidorus. Bayesian 50% majority rule consensus trees as inferred from cytochrome c oxidase subunit I (coxI) mtDNA gene sequence alignments under the GTR + I + G model. Posterior probabilities more than 0.70 are given for appropriate clades. Newly obtained sequences in this study are in bold letters.
Phylogenetic relationships of Longidorus pauli (Lamberti et al., 1999) within the genus Longidorus. Bayesian 50% majority rule consensus trees as inferred from cytochrome c oxidase subunit I (coxI) mtDNA gene sequence alignments under the GTR + I + G model. Posterior probabilities more than 0.70 are given for appropriate clades. Newly obtained sequences in this study are in bold letters.

Morphometrics of Longidorus pauli (Lamberti et al., 1999) from Greece.

Thessaloniki, Greece Idleb, Syria (Lamberti et al., 1999)
Charactera Females Males Females Males
n 8 5 20 12
L (mm) 7.6 ± 0.66 (6.62-8.51) 7.19 ± 0.27 (6.66-8.02) 7.6 ± 0.51 (6.5-8.6) 7.7 ± 0.64 (6.8-8.7)
a 147.9 ± 15.3 (125.0-168.8) 150.1 ± 8.9 (138.3-162.5) 131.6 ± 8.5 (120.3-143.5) 139.9 ± 7.9 (130.8-154.8)
b 16.0 ± 1.7 (13.9-19.0) 16.5 ± 1.3 (14.8-17.8) 16.9 ± 1.6 (14.5-19.7) 16.1 ± 1.8 (12.8-19.5)
c 173.1 ± 29.0 (126.9-224.0) 158.9 ± 12.3 (146.1-175.3) 200.7 ± 17.9 (163.5-220.0) 181.9 ± 11.7 (166.0-197.8)
c’ 1.1 ± 0.1 (1.0-1.3) 1.2 ± 0.1 (1.0-1.3) 0.9 ± 0.1 (0.8-1.0) 0.97 ± 0.05 (0.9-1.0)
d b 2.1 ± 0.2 (1.9-2.4) 2.3 ± 0.2 (2.0-2.6) – –
d’ c 1.6 ± 0.1 (1.4-1.8) 1.5 ± 0.1 (1.4-1.6) – –
V/Spicules length 52.1 ± 2.2 (49.4-56.0) 57.6 ± 0.4 (54.0-60.0) 51 ± 1.5 (49.0-54.0) 64.6 ± 2.5 (61.0-69.0)
Odontostyle length 117.4 ± 5.5 (112.0-126.0) 113.6 ± 7.4 (103.0-120.0) 109.4 ± 3.6 (102.0-118.3) 109.0 ± 4.7 (101.5-117.7)
Odontophore length 63.5 ± 2.2 (61.0-67.0) 63.4 ± 3.3 (59.0.68.0) 61.2 ± 2.5 (56.0-64.0) 63.0 ± 1.8 (61.0-66.5)
Total stylet length 180.9 ± 6.8 (173.0-192.0) 177.0 ± 6.7 (167.0-184.0) – –
Anterior end to guide ring 31.6 ± 1.9 (28.0-33.5) 33.2 ± 1.3 (32.0-35.0) 30.6 ± 2.1 (27.2-35.8) 30.4 ± 1.6 (27.7-32.9)
Tail length 42.9 ± 2.1 (38.0-45.0) 46.6 ± 1.3 (44.0-49.0) 37.8 ± 3.2 (31.5-45.0) 42.4 ± 3.3 (36.4-46.3)
Hyaline part of tail length 16.4 ± 1.5 (14.0-18.5) 14.3 ± 2.0 (12.5-17.0) 13.1 ± 1.1 (10.5-15.4) 11.5 ± 1.6 (10.0-14.9)
Body width at level of
 lip region 14.9 ± 0.7 (13.5-16.0) 14.8 ± 1.0 (13.5-16.0) 15.2 ± 0.9 (14.0-17.0) 14.2 ± 0.5 (13.9-15.0)
 guide ring 23.1 ± 1.5 (21-24.5) 22.6 ± 1.5 (20.0-23.5) 23.5 ± 1.1 (21.0-25.0) 21.8 ± 1.0 (19.6-23.0)
 anus 38.8 ± 1.3 (37.0-41.5) 36.6 ± 1.5 (32.0-39.0) 41.4 ± 2.4 (36.6-44.6) 42.6 ± 2.3 (37.6-45.7)
eISSN:
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