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Morphological and molecular characterisation of Longidorus pauli (Nematoda: Longidoridae), first report from Greece

Emmanuel A. Tzortzakakis

Emmanuel A. Tzortzakakis

Department of Viticulture, Vegetable Crops, Floriculture and Plant Protection, Institute of Olive Tree, Subtropical Crops and Viticulture, N.AG.RE.F., Hellenic Agricultural Organization – DIMITRACrete, Greece
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Tzortzakakis, Emmanuel A.
, 
Ilenia Clavero-Camacho

Ilenia Clavero-Camacho

Institute for Sustainable Agriculture (IAS), CSIC, Avenida Menéndez Pidal s/n, 14004 Córdoba, Campus de Excelencia Internacional AgroalimentarioceiA3, Spain
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Clavero-Camacho, Ilenia
, 
Carolina Cantalapiedra-Navarrete

Carolina Cantalapiedra-Navarrete

Institute for Sustainable Agriculture (IAS), CSIC, Avenida Menéndez Pidal s/n, 14004 Córdoba, Campus de Excelencia Internacional AgroalimentarioceiA3, Spain
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Cantalapiedra-Navarrete, Carolina
, 
Parthenopi Ralli

Parthenopi Ralli

Institute of Plant Breeding and Genetic Resources, N.AG.RE.F., Hellenic Agricultural Organization – DIMITRA, ThermiThessaloniki, Greece
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Ralli, Parthenopi
, 
Juan E. Palomares-Rius

Juan E. Palomares-Rius

Institute for Sustainable Agriculture (IAS), CSIC, Avenida Menéndez Pidal s/n, 14004 Córdoba, Campus de Excelencia Internacional AgroalimentarioceiA3, Spain
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Palomares-Rius, Juan E.
, 
Pablo Castillo

Pablo Castillo

Institute for Sustainable Agriculture (IAS), CSIC, Avenida Menéndez Pidal s/n, 14004 Córdoba, Campus de Excelencia Internacional AgroalimentarioceiA3, Spain
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Castillo, Pablo
 and 
Antonio Archidona-Yuste

Antonio Archidona-Yuste

Department of Ecological Modelling, Helmholtz Centre for Environmental Research – UFZ04318 Leipzig, Germany
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Archidona-Yuste, Antonio
  
Jan 01, 2021
Morphological and molecular characterisation of Longidorus pauli (Nematoda: Longidoridae), first report from Greece's Cover Image
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Published Online: Jan 01, 2021
Page range: 1 - 10
DOI: https://doi.org/10.21307/jofnem-2021-034
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© 2021 Authors, published by Sciendo.
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1:

Light micrographs of Longidorus pauli (Lamberti et al., 1999) from Greece (A-N). A-E: Female anterior regions. F: Detail of base of odontostyle and odontophore, G: Vulval region showing sperm cells in the uterus, H-K: Female tail regions, L: Detail of sperm cells in male genital tract, M: Male tail region, N: Detail of spicules. a = anus; af = amphidial fovea; as = adanal supplement; c = cloaca; gr = guiding ring; Odt = odontostyle; Odp = odontophore; sp = spicules; spc = sperm cells; V = vulva. (Scale bars: A-N = 20 µm).
Light micrographs of Longidorus pauli (Lamberti et al., 1999) from Greece (A-N). A-E: Female anterior regions. F: Detail of base of odontostyle and odontophore, G: Vulval region showing sperm cells in the uterus, H-K: Female tail regions, L: Detail of sperm cells in male genital tract, M: Male tail region, N: Detail of spicules. a = anus; af = amphidial fovea; as = adanal supplement; c = cloaca; gr = guiding ring; Odt = odontostyle; Odp = odontophore; sp = spicules; spc = sperm cells; V = vulva. (Scale bars: A-N = 20 µm).

Figure 2:

Phylogenetic relationships of Longidorus pauli (Lamberti et al., 1999) within the genus Longidorus. Bayesian 50% majority rule consensus tree as inferred from D2 and D3 expansion domains of 28S rRNA sequence alignment under the general time-reversible model of sequence evolution with correction for invariable sites and a gamma-shaped distribution (GTR + I + G). Posterior probabilities more than 0.70 are given for appropriate clades. Newly obtained sequences in this study are shown in bold. Scale bar = expected changes per site.
Phylogenetic relationships of Longidorus pauli (Lamberti et al., 1999) within the genus Longidorus. Bayesian 50% majority rule consensus tree as inferred from D2 and D3 expansion domains of 28S rRNA sequence alignment under the general time-reversible model of sequence evolution with correction for invariable sites and a gamma-shaped distribution (GTR + I + G). Posterior probabilities more than 0.70 are given for appropriate clades. Newly obtained sequences in this study are shown in bold. Scale bar = expected changes per site.

Figure 3:

Phylogenetic relationships of Longidorus pauli (Lamberti et al., 1999) from Greece within the genus Longidorus. Bayesian 50% majority rule consensus tree as inferred from ITS1 rRNA sequence alignment under the TIM3 + G model. Posterior probabilities more than 0.70 are given for appropriate clades. Newly obtained sequences in this study are shown in bold. Scale bar = expected changes per site.
Phylogenetic relationships of Longidorus pauli (Lamberti et al., 1999) from Greece within the genus Longidorus. Bayesian 50% majority rule consensus tree as inferred from ITS1 rRNA sequence alignment under the TIM3 + G model. Posterior probabilities more than 0.70 are given for appropriate clades. Newly obtained sequences in this study are shown in bold. Scale bar = expected changes per site.

Figure 4:

Phylogenetic relationships of Longidorus pauli (Lamberti et al., 1999) within the genus Longidorus. Bayesian 50% majority rule consensus trees as inferred from cytochrome c oxidase subunit I (coxI) mtDNA gene sequence alignments under the GTR + I + G model. Posterior probabilities more than 0.70 are given for appropriate clades. Newly obtained sequences in this study are in bold letters.
Phylogenetic relationships of Longidorus pauli (Lamberti et al., 1999) within the genus Longidorus. Bayesian 50% majority rule consensus trees as inferred from cytochrome c oxidase subunit I (coxI) mtDNA gene sequence alignments under the GTR + I + G model. Posterior probabilities more than 0.70 are given for appropriate clades. Newly obtained sequences in this study are in bold letters.

Morphometrics of Longidorus pauli (Lamberti et al_, 1999) from Greece_

Thessaloniki, Greece Idleb, Syria (Lamberti et al., 1999)
Charactera Females Males Females Males
n 8 5 20 12
L (mm) 7.6 ± 0.66 (6.62-8.51) 7.19 ± 0.27 (6.66-8.02) 7.6 ± 0.51 (6.5-8.6) 7.7 ± 0.64 (6.8-8.7)
a 147.9 ± 15.3 (125.0-168.8) 150.1 ± 8.9 (138.3-162.5) 131.6 ± 8.5 (120.3-143.5) 139.9 ± 7.9 (130.8-154.8)
b 16.0 ± 1.7 (13.9-19.0) 16.5 ± 1.3 (14.8-17.8) 16.9 ± 1.6 (14.5-19.7) 16.1 ± 1.8 (12.8-19.5)
c 173.1 ± 29.0 (126.9-224.0) 158.9 ± 12.3 (146.1-175.3) 200.7 ± 17.9 (163.5-220.0) 181.9 ± 11.7 (166.0-197.8)
c’ 1.1 ± 0.1 (1.0-1.3) 1.2 ± 0.1 (1.0-1.3) 0.9 ± 0.1 (0.8-1.0) 0.97 ± 0.05 (0.9-1.0)
d b 2.1 ± 0.2 (1.9-2.4) 2.3 ± 0.2 (2.0-2.6) – –
d’ c 1.6 ± 0.1 (1.4-1.8) 1.5 ± 0.1 (1.4-1.6) – –
V/Spicules length 52.1 ± 2.2 (49.4-56.0) 57.6 ± 0.4 (54.0-60.0) 51 ± 1.5 (49.0-54.0) 64.6 ± 2.5 (61.0-69.0)
Odontostyle length 117.4 ± 5.5 (112.0-126.0) 113.6 ± 7.4 (103.0-120.0) 109.4 ± 3.6 (102.0-118.3) 109.0 ± 4.7 (101.5-117.7)
Odontophore length 63.5 ± 2.2 (61.0-67.0) 63.4 ± 3.3 (59.0.68.0) 61.2 ± 2.5 (56.0-64.0) 63.0 ± 1.8 (61.0-66.5)
Total stylet length 180.9 ± 6.8 (173.0-192.0) 177.0 ± 6.7 (167.0-184.0) – –
Anterior end to guide ring 31.6 ± 1.9 (28.0-33.5) 33.2 ± 1.3 (32.0-35.0) 30.6 ± 2.1 (27.2-35.8) 30.4 ± 1.6 (27.7-32.9)
Tail length 42.9 ± 2.1 (38.0-45.0) 46.6 ± 1.3 (44.0-49.0) 37.8 ± 3.2 (31.5-45.0) 42.4 ± 3.3 (36.4-46.3)
Hyaline part of tail length 16.4 ± 1.5 (14.0-18.5) 14.3 ± 2.0 (12.5-17.0) 13.1 ± 1.1 (10.5-15.4) 11.5 ± 1.6 (10.0-14.9)
Body width at level of
 lip region 14.9 ± 0.7 (13.5-16.0) 14.8 ± 1.0 (13.5-16.0) 15.2 ± 0.9 (14.0-17.0) 14.2 ± 0.5 (13.9-15.0)
 guide ring 23.1 ± 1.5 (21-24.5) 22.6 ± 1.5 (20.0-23.5) 23.5 ± 1.1 (21.0-25.0) 21.8 ± 1.0 (19.6-23.0)
 anus 38.8 ± 1.3 (37.0-41.5) 36.6 ± 1.5 (32.0-39.0) 41.4 ± 2.4 (36.6-44.6) 42.6 ± 2.3 (37.6-45.7)
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