Otwarty dostęp

Evaluation of root-knot nematode resistance assays for sugarcane accession lines in Australia

S. A. Bhuiyan
S. A. Bhuiyan
 oraz 
K. Garlick
K. Garlick
   | 01 sty 2021
Journal of Nematology's Cover Image
O artykule
Poprzedni artykuł
Następny artykuł
Zacytuj
Data publikacji: 01 sty 2021
Zakres stron: 1 - 11
DOI: https://doi.org/10.21307/jofnem-2021-006
Słowa kluczowe
© 2021 Authors, published by Sciendo.
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1:

Examples of root-knot nematode gall ratings for sugarcane based on percentage of root system with galls. 1 = ≤1 to 2%, 2 = 2 to 25%, 3 = 25 to 50%, 4 = 51 to 75%, 5 ≥ 75% (modified from Shepherd 1979).
Examples of root-knot nematode gall ratings for sugarcane based on percentage of root system with galls. 1 = ≤1 to 2%, 2 = 2 to 25%, 3 = 25 to 50%, 4 = 51 to 75%, 5 ≥ 75% (modified from Shepherd 1979).

Figure 2:

Regressions to show the relationship between root biomass and ln(eggs per g roots+1) in eight nematode trials.
Regressions to show the relationship between root biomass and ln(eggs per g roots+1) in eight nematode trials.

Pearson correlation coefficients among 22 accession lines to measure the repeatability among trials in relation to nematode (Meloidogyne javanica) resistance parameters.

Trial by Trial No. of accession linesa Eggs per plantb Eggs per g root Gall rating
Nem12-1 Nem13-1 6 0.99*** 0.89* 0.92**
Nem12-1 Nem13-2 6 0.98*** 0.94** 0.86*
Nem12-1 Nem15-1 6 0.10 ns 0.20 ns 0.56 ns
Nem12-1 Nem16-2 7 0.98*** 0.76* 0.20 ns
Nem12-1 Nem17-1 6 0.94** 0.95** 0.86*
Nem12-1 Nem18-1 8 0.93** 0.63 ns 0.64 ns
Nem12-1 Nem19-1 8 0.87** 0.92** 0.86**
Nem13-1 Nem13-2 3 0.99 ns 0.82 ns –
Nem13-1 Nem15-1 12 0.47 ns 0.31 ns 0.27 ns
Nem13-1 Nem16-2 12 0.92*** 0.10 ns 0.35 ns
Nem13-1 Nem17-1 10 0.74* 0.60 ns 0.72*
Nem13-1 Nem18-1 8 0.91** 0.71* 0.82*
Nem13-1 Nem19-1 7 0.69 ns 0.59 ns 0.89**
Nem13-2 Nem15-1 5 −0.02 ns 0.33 ns 0.71 ns
Nem13-2 Nem16-2 6 0.95** 0.80 ns −0.30 ns
Nem13-2 Nem17-1 5 0.92* 0.95* 0.68 ns
Nem13-2 Nem18-1 6 0.90* 0.46 ns 0.68 ns
Nem13-2 Nem19-1 6 0.86* 0.83* 0.87*
Nem15-1 Nem16-2 18 0.19 ns 0.29 ns −0.30 ns
Nem15-1 Nem17-1 15 0.25 ns 0.12 ns 0.19 ns
Nem15-1 Nem18-1 10 0.51 ns 0.66* 0.16 ns
Nem15-1 Nem19-1 13 0.66* 0.60* -0.48 ns
Nem16-2 Nem17-1 14 0.74** 0.28 ns 0.06 ns
Nem16-2 Nem18-1 10 0.81 ns 0.63* −0.38 ns
Nem16-2 Nem19-1 12 0.43 ns 0.40 ns 0.27 ns
Nem17-1 Nem18-1 8 0.97*** 0.77* 0.82**
Nem17-1 Nem19-1 13 0.92*** 0.86*** 0.79**
Nem18-1 Nem19-1 10 0.93*** 0.71* 0.88**

Pearson correlation coefficients to compare relationships of nematode (Meloidogyne javanica) resistance parameters with sugarcane shoot biomass and root biomass.

Eggs per plant Eggs per g root Visual rating
Trial name Shoot biomass Root biomass Shoot biomass Root biomass Shoot biomass Root biomass
Nem19-1 −0.08 nsa −0.27 ns −0.47** −0.74*** −0.14 ns −0.41**
Nem18-1 0.01 ns −0.03 ns −0.36** −0.45** 0.01 ns −0.08 ns
Nem17-1 0.070 ns −0.26 ns −0.42** −0.65*** −0.004 ns −0.26 ns
Nem16-2 – 0.13 ns – −0.44*** – 0.11 ns
Nem15-1 −0.093 ns −0.07 ns −0.24** −0.28** −0.19 ns −0.12 ns
Nem13-1 0.15 ns 0.14 ns 0.12 ns −0.06 ns 0.30** 0.21*
Nem13-2 0.19 ns 0.02 ns 0.08 ns −0.19* −0.10 ns −0.40***
Nem12-1 0.18 ns 0.06 ns −0.02 ns −0.23** 0.24* −0.04 ns

Trial names, trial year, number of accession lines and types of sugarcane populations screened for root-knot nematode (Meloidogyne javanica) resistance from 2012 to 2019.

Trial name Trial year Number of accession lines Source
Nem19-1 2019 42 Core breeding program
Nem18-1 2018 56 Core breeding program
Nem17-1 2017 56 Core breeding program
Nem16-2 2016 95 Introgression population
Nem15-1 2015 112 Introgression population
Nem13-1 2013 138 Introgression population
Nem13-2 2013 155 Introgression population
Nem12-1 2012 148 Introgression population
Total accession lines – 802 –

Coefficient of variance (%) among shoot biomass, root biomass, visual ratings, number of eggs per plant and number of eggs per g of roots in each trial.

Trial name Shoot biomass Root biomass Visual rating Eggs per plant Eggs per g root
Nem19-1 21.5 36.3 20.5 2.3 6.4
Nem18-1 20.7 38.7 19.3 2.8 3.2
Nem17-1 27.3 48.7 20.5 2.7 9.9
Nem16-2 - 38.2 22.1 6.0 8.7
Nem15-1 20.9 38.3 30.0 9.2 11.9
Nem13-1 21.5 29.6 22.4 28.6 47.1
Nem13-2 27.9 37.0 22.6 25.3 27.8
Nem12-1 24.0 37.5 29.3 25.0 25.5

List of 22 sugarcane accession lines used in multiple trials with root biomass, and nematode resistance parameters.

Accession Type No. of trialsa Root biomass (g) Relative eggs per plante (%) Relative eggs per g root (%) Visual gall rating
IJ76-333 Erianthus spb 5 25.3±12.5d 12.4±2.19 25.6±18.99 1.3±0.06
IJ76-370 Erianthus sp 5 23.2±11.16 10.7±1.19 7.8±3.26 1.1±0.1
KQ228 Core 8 21.0±7.66 43.2±6.62 23.6±9.1 2.6±0.17
Q135c Core 7 12.9±4.78 125.2±27.71 145.0±67.32 3.2±0.2
Q138 Core 7 15.2±4.61 40.0±8.68 25.5±10.19 2.7±0.35
Q183 Core 4 6.8±4.14 67.8±11.13 74.9±37.44 3.0±0.19
Q200 Core 6 10.9±5.07 144.2±43.27 115.7±40.94 2.9±0.2
Q208c Core 8 16.2±5.02 77.9±8.56 60.6±28.92 3.0±0.23
Q231 Core 4 17.6±12.07 36.4±14.26 16.6±10.16 3.0±0.51
Q232 Core 6 14.3±5.13 55.5±14.06 43.8±21.41 2.7±0.31
Q238 Core 4 16.2±7.96 53.5±14.84 25.8±12.79 3.4±0.18
Q240 Core 5 20.4±6.38 101.4±25.49 29.0±13.63 2.8±0.3
Q241 Core 4 8.0±6.59 30.8±8.73 88.1±49.45 3.1±0.43
Q242 Core 4 11.8±6.31 64.6±28.2 30.5±14.41 3.0±0.57
Q245 Core 6 9.4±4.78 45.7±7.38 58.9±28.38 2.7±0.25
Q248 Core 4 16.2±8.99 139.2±35.96 51.5±24.73 3.3±0.28
Q249 Core 4 13.1±7.65 24.6±3.16 17.0±10.41 3.0±0.34
Q251 Core 4 12.6±4.82 90.1±21.37 30.7±11.44 2.7±0.37
QBYN05-20563 Introgression 4 24.1±3.03 11.7±3.4 3.7±1.83 2.6±0.22
SRA1 Core 4 6.2±3.65 43.2±11.22 81.7±57.66 2.7±0.24
SRA2 Core 4 3.5±1.66 43.9±12.05 79.9±46.09 2.4±0.14
SRA3 Core 4 8.5±5.13 60.7±14.42 89.0±63.14 2.9±0.4
eISSN:
2640-396X
Język:
Angielski
Częstotliwość wydawania:
Volume Open
Dziedziny czasopisma:
Life Sciences, other
Kanał RSS czasopisma