Evaluation of root-knot nematode resistance assays for sugarcane accession lines in Australia

Figure 1:

Figure 2:

Pearson correlation coefficients among 22 accession lines to measure the repeatability among trials in relation to nematode (Meloidogyne javanica) resistance parameters_

Trial	by Trial	No. of accession linesa	Eggs per plantb	Eggs per g root	Gall rating
Nem12-1	Nem13-1	6	0.99***	0.89*	0.92**
Nem12-1	Nem13-2	6	0.98***	0.94**	0.86*
Nem12-1	Nem15-1	6	0.10 ns	0.20 ns	0.56 ns
Nem12-1	Nem16-2	7	0.98***	0.76*	0.20 ns
Nem12-1	Nem17-1	6	0.94**	0.95**	0.86*
Nem12-1	Nem18-1	8	0.93**	0.63 ns	0.64 ns
Nem12-1	Nem19-1	8	0.87**	0.92**	0.86**
Nem13-1	Nem13-2	3	0.99 ns	0.82 ns	–
Nem13-1	Nem15-1	12	0.47 ns	0.31 ns	0.27 ns
Nem13-1	Nem16-2	12	0.92***	0.10 ns	0.35 ns
Nem13-1	Nem17-1	10	0.74*	0.60 ns	0.72*
Nem13-1	Nem18-1	8	0.91**	0.71*	0.82*
Nem13-1	Nem19-1	7	0.69 ns	0.59 ns	0.89**
Nem13-2	Nem15-1	5	−0.02 ns	0.33 ns	0.71 ns
Nem13-2	Nem16-2	6	0.95**	0.80 ns	−0.30 ns
Nem13-2	Nem17-1	5	0.92*	0.95*	0.68 ns
Nem13-2	Nem18-1	6	0.90*	0.46 ns	0.68 ns
Nem13-2	Nem19-1	6	0.86*	0.83*	0.87*
Nem15-1	Nem16-2	18	0.19 ns	0.29 ns	−0.30 ns
Nem15-1	Nem17-1	15	0.25 ns	0.12 ns	0.19 ns
Nem15-1	Nem18-1	10	0.51 ns	0.66*	0.16 ns
Nem15-1	Nem19-1	13	0.66*	0.60*	-0.48 ns
Nem16-2	Nem17-1	14	0.74**	0.28 ns	0.06 ns
Nem16-2	Nem18-1	10	0.81 ns	0.63*	−0.38 ns
Nem16-2	Nem19-1	12	0.43 ns	0.40 ns	0.27 ns
Nem17-1	Nem18-1	8	0.97***	0.77*	0.82**
Nem17-1	Nem19-1	13	0.92***	0.86***	0.79**
Nem18-1	Nem19-1	10	0.93***	0.71*	0.88**

Pearson correlation coefficients to compare relationships of nematode (Meloidogyne javanica) resistance parameters with sugarcane shoot biomass and root biomass_

	Eggs per plant		Eggs per g root		Visual rating
Trial name	Shoot biomass	Root biomass	Shoot biomass	Root biomass	Shoot biomass	Root biomass
Nem19-1	−0.08 nsa	−0.27 ns	−0.47**	−0.74***	−0.14 ns	−0.41**
Nem18-1	0.01 ns	−0.03 ns	−0.36**	−0.45**	0.01 ns	−0.08 ns
Nem17-1	0.070 ns	−0.26 ns	−0.42**	−0.65***	−0.004 ns	−0.26 ns
Nem16-2	–	0.13 ns	–	−0.44***	–	0.11 ns
Nem15-1	−0.093 ns	−0.07 ns	−0.24**	−0.28**	−0.19 ns	−0.12 ns
Nem13-1	0.15 ns	0.14 ns	0.12 ns	−0.06 ns	0.30**	0.21*
Nem13-2	0.19 ns	0.02 ns	0.08 ns	−0.19*	−0.10 ns	−0.40***
Nem12-1	0.18 ns	0.06 ns	−0.02 ns	−0.23**	0.24*	−0.04 ns

Trial names, trial year, number of accession lines and types of sugarcane populations screened for root-knot nematode (Meloidogyne javanica) resistance from 2012 to 2019_

Trial name	Trial year	Number of accession lines	Source
Nem19-1	2019	42	Core breeding program
Nem18-1	2018	56	Core breeding program
Nem17-1	2017	56	Core breeding program
Nem16-2	2016	95	Introgression population
Nem15-1	2015	112	Introgression population
Nem13-1	2013	138	Introgression population
Nem13-2	2013	155	Introgression population
Nem12-1	2012	148	Introgression population
Total accession lines	–	802	–

Coefficient of variance (%) among shoot biomass, root biomass, visual ratings, number of eggs per plant and number of eggs per g of roots in each trial_

Trial name	Shoot biomass	Root biomass	Visual rating	Eggs per plant	Eggs per g root
Nem19-1	21.5	36.3	20.5	2.3	6.4
Nem18-1	20.7	38.7	19.3	2.8	3.2
Nem17-1	27.3	48.7	20.5	2.7	9.9
Nem16-2	-	38.2	22.1	6.0	8.7
Nem15-1	20.9	38.3	30.0	9.2	11.9
Nem13-1	21.5	29.6	22.4	28.6	47.1
Nem13-2	27.9	37.0	22.6	25.3	27.8
Nem12-1	24.0	37.5	29.3	25.0	25.5

List of 22 sugarcane accession lines used in multiple trials with root biomass, and nematode resistance parameters_

Accession	Type	No. of trialsa	Root biomass (g)	Relative eggs per plante (%)	Relative eggs per g root (%)	Visual gall rating
IJ76-333	Erianthus spb	5	25.3±12.5d	12.4±2.19	25.6±18.99	1.3±0.06
IJ76-370	Erianthus sp	5	23.2±11.16	10.7±1.19	7.8±3.26	1.1±0.1
KQ228	Core	8	21.0±7.66	43.2±6.62	23.6±9.1	2.6±0.17
Q135c	Core	7	12.9±4.78	125.2±27.71	145.0±67.32	3.2±0.2
Q138	Core	7	15.2±4.61	40.0±8.68	25.5±10.19	2.7±0.35
Q183	Core	4	6.8±4.14	67.8±11.13	74.9±37.44	3.0±0.19
Q200	Core	6	10.9±5.07	144.2±43.27	115.7±40.94	2.9±0.2
Q208c	Core	8	16.2±5.02	77.9±8.56	60.6±28.92	3.0±0.23
Q231	Core	4	17.6±12.07	36.4±14.26	16.6±10.16	3.0±0.51
Q232	Core	6	14.3±5.13	55.5±14.06	43.8±21.41	2.7±0.31
Q238	Core	4	16.2±7.96	53.5±14.84	25.8±12.79	3.4±0.18
Q240	Core	5	20.4±6.38	101.4±25.49	29.0±13.63	2.8±0.3
Q241	Core	4	8.0±6.59	30.8±8.73	88.1±49.45	3.1±0.43
Q242	Core	4	11.8±6.31	64.6±28.2	30.5±14.41	3.0±0.57
Q245	Core	6	9.4±4.78	45.7±7.38	58.9±28.38	2.7±0.25
Q248	Core	4	16.2±8.99	139.2±35.96	51.5±24.73	3.3±0.28
Q249	Core	4	13.1±7.65	24.6±3.16	17.0±10.41	3.0±0.34
Q251	Core	4	12.6±4.82	90.1±21.37	30.7±11.44	2.7±0.37
QBYN05-20563	Introgression	4	24.1±3.03	11.7±3.4	3.7±1.83	2.6±0.22
SRA1	Core	4	6.2±3.65	43.2±11.22	81.7±57.66	2.7±0.24
SRA2	Core	4	3.5±1.66	43.9±12.05	79.9±46.09	2.4±0.14
SRA3	Core	4	8.5±5.13	60.7±14.42	89.0±63.14	2.9±0.4