Morphological and Molecular Characterization of Oscheius saproxylicus sp. n. (Rhabditida, Rhabditidae) From Decaying Wood in Spain, With New Insights into the Phylogeny of the Genus and a Revision of its Taxonomy
Oscheius saproxylicus sp. n. (light microscopy, female). (A) Neck; (B-D) Stoma in lateral (B, C) and dorso-ventral (D) views; (E, N) Reproductive system (arrow at spermatozoa); (F, G) Lip region in lateral and ventral views, respectively; (H) Lateral field; (I, J) Posterior end with rectum empty and swollen, respectively; (K) Vagina; (L) Excretory pore in ventral view (white arrow) and deirids (black arrow); (M) Intestine cell with microsporidia (arrow).
Figure 3:
Oscheius saproxylicus sp. n. (scanning electron microscopy, female). (A) Entire body; (B, D) Lip region in subfrontal and frontal views, respectively (black arrows at amphid and white arrows pointing the metastegostomatal teeth, up: dorsal tooth, down: sublateral teeth); (C) Cuticle at midbody; E: Excretory pore (black arrow) and deirid (white arrow); (F, J, K) Vulval region at ventral, sublateral right and left views, respectively (arrows at lineal warts of the lateral fields); (G, H) Tail in ventral and lateral views, respectively (arrow at phasmid); (I) Lateral field.
Figure 4:
Bayesian Inference tree from known and the newly sequenced Oscheius saproxylicus sp. n. based on sequences of the 18S rDNA region. Bayesian posterior probabilities (%) are given for each clade. Scale bar shows the number of substitutions per site.
Figure 5:
Bayesian Inference tree from known and the newly sequenced Oscheius saproxylicus sp. n. based on sequences of the 28S rDNA region. Bayesian posterior probabilities (%) are given for each clade. Scale bar shows the number of substitutions per site.
Figure 6:
Lip region, male and female posterior ends of the species of the subgenus Oscheius (Andrássy, 1976) (not to scale). Based in the original illustrations (line drawings and LM pictures) except: isolated spicule in O. lucianii (cf. Chitwood, 1933).
Figure 7:
Lip region, male and female posterior ends of the species of the subgenus Dolichorhabditis (Andrássy, 1983) (not to scale). Based on the original illustrations (line drawings and LM pictures) except: O. bengalensis (cf. Sudhaus, 1974), O. dolichura (cf. Andrássy, 2005), lip region in O. sechellensis (cf. Sudhaus,1976), O. tipulae (cf. Abolafia and Peña-Santiago, 2010).