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Morphological and Molecular Characterization of Oscheius saproxylicus sp. n. (Rhabditida, Rhabditidae) From Decaying Wood in Spain, With New Insights into the Phylogeny of the Genus and a Revision of its Taxonomy

Joaquín Abolafia
Joaquín Abolafia
 and 
Reyes Peña-Santiago
Reyes Peña-Santiago
   | Sep 17, 2019
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Article Category: Arts & Humanities
Published Online: Sep 17, 2019
Page range: 1 - 21
Received: Nov 13, 2018
DOI: https://doi.org/10.21307/jofnem-2019-053
© 2019 Joaquín Abolafia et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1:

Oscheius saproxylicus sp. n. (line drawing). (A) Neck; (B): Entire female; (C) Stoma; (D) Lip region; (E) Genital system; (F) Vagina; (G, H) Female tail.
Oscheius saproxylicus sp. n. (line drawing). (A) Neck; (B): Entire female; (C) Stoma; (D) Lip region; (E) Genital system; (F) Vagina; (G, H) Female tail.

Figure 2:

Oscheius saproxylicus sp. n. (light microscopy, female). (A) Neck; (B-D) Stoma in lateral (B, C) and dorso-ventral (D) views; (E, N) Reproductive system (arrow at spermatozoa); (F, G) Lip region in lateral and ventral views, respectively; (H) Lateral field; (I, J) Posterior end with rectum empty and swollen, respectively; (K) Vagina; (L) Excretory pore in ventral view (white arrow) and deirids (black arrow); (M) Intestine cell with microsporidia (arrow).
Oscheius saproxylicus sp. n. (light microscopy, female). (A) Neck; (B-D) Stoma in lateral (B, C) and dorso-ventral (D) views; (E, N) Reproductive system (arrow at spermatozoa); (F, G) Lip region in lateral and ventral views, respectively; (H) Lateral field; (I, J) Posterior end with rectum empty and swollen, respectively; (K) Vagina; (L) Excretory pore in ventral view (white arrow) and deirids (black arrow); (M) Intestine cell with microsporidia (arrow).

Figure 3:

Oscheius saproxylicus sp. n. (scanning electron microscopy, female). (A) Entire body; (B, D) Lip region in subfrontal and frontal views, respectively (black arrows at amphid and white arrows pointing the metastegostomatal teeth, up: dorsal tooth, down: sublateral teeth); (C) Cuticle at midbody; E: Excretory pore (black arrow) and deirid (white arrow); (F, J, K) Vulval region at ventral, sublateral right and left views, respectively (arrows at lineal warts of the lateral fields); (G, H) Tail in ventral and lateral views, respectively (arrow at phasmid); (I) Lateral field.
Oscheius saproxylicus sp. n. (scanning electron microscopy, female). (A) Entire body; (B, D) Lip region in subfrontal and frontal views, respectively (black arrows at amphid and white arrows pointing the metastegostomatal teeth, up: dorsal tooth, down: sublateral teeth); (C) Cuticle at midbody; E: Excretory pore (black arrow) and deirid (white arrow); (F, J, K) Vulval region at ventral, sublateral right and left views, respectively (arrows at lineal warts of the lateral fields); (G, H) Tail in ventral and lateral views, respectively (arrow at phasmid); (I) Lateral field.

Figure 4:

Bayesian Inference tree from known and the newly sequenced Oscheius saproxylicus sp. n. based on sequences of the 18S rDNA region. Bayesian posterior probabilities (%) are given for each clade. Scale bar shows the number of substitutions per site.
Bayesian Inference tree from known and the newly sequenced Oscheius saproxylicus sp. n. based on sequences of the 18S rDNA region. Bayesian posterior probabilities (%) are given for each clade. Scale bar shows the number of substitutions per site.

Figure 5:

Bayesian Inference tree from known and the newly sequenced Oscheius saproxylicus sp. n. based on sequences of the 28S rDNA region. Bayesian posterior probabilities (%) are given for each clade. Scale bar shows the number of substitutions per site.
Bayesian Inference tree from known and the newly sequenced Oscheius saproxylicus sp. n. based on sequences of the 28S rDNA region. Bayesian posterior probabilities (%) are given for each clade. Scale bar shows the number of substitutions per site.

Figure 6:

Lip region, male and female posterior ends of the species of the subgenus Oscheius (Andrássy, 1976) (not to scale). Based in the original illustrations (line drawings and LM pictures) except: isolated spicule in O. lucianii (cf. Chitwood, 1933).
Lip region, male and female posterior ends of the species of the subgenus Oscheius (Andrássy, 1976) (not to scale). Based in the original illustrations (line drawings and LM pictures) except: isolated spicule in O. lucianii (cf. Chitwood, 1933).

Figure 7:

Lip region, male and female posterior ends of the species of the subgenus Dolichorhabditis (Andrássy, 1983) (not to scale). Based on the original illustrations (line drawings and LM pictures) except: O. bengalensis (cf. Sudhaus, 1974), O. dolichura (cf. Andrássy, 2005), lip region in O. sechellensis (cf. Sudhaus,1976), O. tipulae (cf. Abolafia and Peña-Santiago, 2010).
Lip region, male and female posterior ends of the species of the subgenus Dolichorhabditis (Andrássy, 1983) (not to scale). Based on the original illustrations (line drawings and LM pictures) except: O. bengalensis (cf. Sudhaus, 1974), O. dolichura (cf. Andrássy, 2005), lip region in O. sechellensis (cf. Sudhaus,1976), O. tipulae (cf. Abolafia and Peña-Santiago, 2010).
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