Survival and Infectivity of Entomopathogenic Nematodes Formulated in Sodium Alginate Beads
, oraz | 17 paź 2018
O artykule
Data publikacji: 17 paź 2018
Zakres stron: 273 - 280
DOI: https://doi.org/10.21307/jofnem-2018-037
Słowa kluczowe
© 2018 Jaime Ruiz-Vega et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.
Survival over time of entomopathogenic nematodes (EPNs) formulated in sodium alginate beads (ABs) in relation to the pre-conditioning (Prec) of IJs or ABs coating (C) or both (Prec-C), stored at 23 ± 3°C. Steinernema glaseri (Sg), Steinernema carpocapsae (Sc), and Heterorhabditis bacteriophora (Hb).
| Survival rate after formulation (%) | |||||||
|---|---|---|---|---|---|---|---|
| Factor | Treatment | 7 d | 14 d | 21 d | 28 d | 35 d | MSt (days ± SE) |
| EPN |
|
15 | 0 | 0 | 0 | 0 | 8.05 ± 0.079 |
|
|
79 | 79 | 58.8 | 58.8 | 0 | 26.07 ± 0.389 | |
|
|
93.5 | 93.5 | 51.4 | 0 | 0 | 22.00 ± 0.209 | |
| Prec |
|
39.6 | 39.6 | 22.5 | 0 | 0 | 13.21 ± 0.262 |
|
|
53.3 | 23 | 9.8 | 0 | 0 | 13.02 ± 0.218 | |
|
|
69.3 | 69.3 | 7.6 | 0 | 0 | 14.69 ± 0.182 | |
| C |
|
20 | 0 | 0 | 0 | 0 | 8.40 ± 0.088 |
|
|
79 | 79 | 41.6 | 41.6 | 0 | 26.96 ± 0.355 | |
|
|
100 | 100 | 30.5 | 21.7 | 0 | 22.22 ± 0.262 | |
| Prec-C |
|
40 | 0 | 0 | 0 | 0 | 9.80 ± 0.108 |
|
|
74.4 | 67.8 | 31.5 | 31.5 | 10 | 22.06 ± 0.372 | |
|
|
92.4 | 0 | 0 | 0 | 0 | 13.46 ± 0.058 | |
Results of the statistical analysis over time of infectivity (IN) against Galleria mellonella by EPNs from sodium ABs, in relation to the pre-conditioning (Prec) of IJs or ABs coating (C) or both (Prec-C). Steinernema glaseri (Sg), Steinernema carpocapsae (Sc), and Heterorhabditis bacteriophora (Hb).
| Infectivity after formulation (%)a | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Factor | Treatment | 7 d | 14 d | 21 d | 28 d | 35 d | Mean IN (% ± SE) |
|
|
| EPN |
|
15b | 0b | 0b | 0b | 0b | 3 ± 2.19bc | 6.28 | 0.023 |
|
|
100a | 100a | 100a | 100a | 0a | 80 ± 9.18a | |||
|
|
90a | 100a | 0b | 0b | 0a | 38 ± 10.8ac | |||
| Prec |
|
75a | 70a | 0a | 0a | 0a | 29 ± 9.5bc | 5.79 | 0.006 |
|
|
100a | 100a | 55a | 0a | 0a | 51 ± 11.3a | |||
|
|
100a | 90a | 0a | 0a | 0a | 38 ± 10.8ac | |||
| C |
|
35b | 0b | 0b | 0b | 0a | 7 ± 3.3b | 219.59 | <0.001 |
|
|
100a | 100a | 100a | 80a | 0a | 76 ± 9.58a | |||
|
|
100a | 100a | 90a | 95a | 0a | 77 ± 9a | |||
| Prec-C |
|
40b | 10b | 0b | 00b | 0b | 10 ± 5.7b | 160.54 | <0.001 |
|
|
100a | 100a | 90a | 90a | 100a | 96 ± 2.7a | |||
|
|
90a | 10b | 0b | 0b | 0b | 20 ± 8.46b | |||