Description of  sp. n. (Nematoda: Longidoridae) from Imported Ornamental Plants in Japan with a Key to  Species in Group 1

Munawar Maria; Weimin Ye; Qing Yu; Jianfeng Gu

Otwarty dostęp

Description of Xiphinema parachambersi sp. n. (Nematoda: Longidoridae) from Imported Ornamental Plants in Japan with a Key to Xiphinema Species in Group 1

oraz

| 17 paź 2018

Journal of Nematology

Tom 50 (2018): Zeszyt 3 (January 2018)

O artykule

Poprzedni artykuł

Następny artykuł

Zacytuj

Udostępnij

Data publikacji: 17 paź 2018

Zakres stron: 369 - 386

DOI: https://doi.org/10.21307/jofnem-2018-027

Słowa kluczowe
Molecular, morphology, morphometrics, nematode, new species

© 2018 Munawar Maria et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Line drawings of Xiphinema parachamberi n. sp. female. A: Esophageal region; B: Esophageal bulb; C: Entire body; D: Genital branch; E: Tail region (Scale bars: A, E = 40; B, C=20, D=100 μm).

Light photomicrographs of Xiphinema parachamberi n. sp. female. A: Anterior region, arrow showing position of vulva (v); B: Lip region; C-E: Esophageal bulb (arrows showing different position of gland nuclei); F: Esophago-intestinal junction arrows pointing the base of pharyngeal bulb and cardia. G: Vulval region; H, I: Gonad; J: Female tail (arrows showing position of (a) anus and caudal pores); K, L: Female tail (arrows showing position of (a) anus) (Scale bars: A, L = 10 μm).

Light photomicrographs of cardia of Xiphinema parachamberi n. sp. Scale bars: A–C=20 μm).

Light photomicrographs of Xiphinema parachamberi n. sp. juveniles and female. A–D: Anterior regions of J2/J3, J3/J4 and female; E–H: Tail regions of J2/J3, J3/J4 and female (arrows showing position of anus (a)) (Scale bars: A–L = 10 μm).

Relationship of body length to length of functional and replacement odontostyle (Ost and rOst, respectively); length in two juvenile developmental stages and mature females of Xiphinema parachambersi n. sp.

Bayesian consensus tree of Xiphinema parachamberi n. sp. inferred from 18S under GTR+I+G model (-lnL=3332.0762; AIC=6682.1523; freqA=0.1821; freqC=0.209; freqG=0.3285; freqT=0.2804; R(a)=1.4406; R(b)=5.2581; R(c)=2.6656; R(d)=0.7382; R(e)=5.2581; R(f)=1; Pinva=0.2316; Shape=0.4544). Posterior probability values exceeding 50% are given on appropriate clades.

Bayesian consensus tree of Xiphinema parachamberi n. sp. inferred from 28S D2/D3 under GTR+I+G model (-lnL=10912.0693; AIC=21844.1387; freqA=0.2463; freqC=0.2277; freqG=0.2969; freqT=0.2291; R(a)=0.9039; R(b)=2.4909; R(c)=2.4092; R(d)=0.4557; R(e)=3.9274; R(f)=1; Pinva=0.3185; Shape=0.782). Posterior probability values exceeding 50% are given on appropriate clades.

Bayesian consensus tree of Xiphinema parachamberi n. sp. inferred from ITS1 under GTR+I+G model (-lnL=19846.0566; AIC=39712.1133; freqA=0.2723; freqC=0.2059; freqG=0.2672; freqT=0.2547; R(a)=0.851; R(b)=3.6772; R(c)=1.3471; R(d)=0.6487; R(e)=5.209; R(f)=1; Pinva=0.0808; Shape=1.2786). Posterior probability values exceeding 50% are given on appropriate clades.

Morphometrics data for Xiphinema parachambersi n. sp. All measurements are in µm and in the form of mean ± s.d. (range).

	Holotype		Paratype
Character/ratios	Female	Female	J2 or J3	J3 or J4
n	1	15	5	11
L	1,918	2008.9±78.7 (1,830.0-2,109.0)	1,127.0±52.6 (1,046.0-1,210.0)	1,419.6±38.5 (1,349.0-1,587.0)
a	45.6	47.2±2.3 (44.2-53.1)	42.7±3.6 (36.7-46.3)	48.3±3.5 (41.60-55.5)
b	5.4	5.5±0.2 (5.1-6.0)	3.9±0.5 (3.0-4.4)	4.7±0.3 (4.2-5.0)
c	18.1	18.0±1.0 (16.1-19.6)	11.8±1.0 (10.5-12.8)	13.2±0.6 (12.0-15.1)
c’	4.4	4.9±0.4 (4.2-6.0)	6.4±0.3 (5.9-6.8)	5.9±0.4 (5.2-6.9)
V	27	26.2±0.6 (25.2-27.7)	—	—
Lip diam.	10	10.3±0.6 (8.8-11.2)	8.6±0.3 (8.2-9.0)	8.9±0.5 (8.2-10.1)
Lip height	5	4.1±0.7 (2.9-5.2)	3.6±0.4 (3.0-4.2)	3.8±0.4 (3.1-4.7)
Odontostyle	115	110.4±2.6 (105-115.6)	71.0±3.8 (64.4-75.6)	85.5±2.0 (79.5-90.0)
Odontophore	67	65.5±1.6 (61.0-68.1)	46.1±1.4 (44.5-47.8)	53.8±1.0 (52.2-54.8)
Replacement odontostyle	—	—	88.0±2.5 (85.0-92.0)	107.0±7.9 (90.5-116.3)
Total stylet	173	176±2.9 (169.0-181.2)	116.6±4.7 (109.0-120.5)	140.0±2.2 (137.2-142.5)
Flanges width	10	10.4±0.7 (8.8-11.5)	8.1±0.4 (7.7-8.7)	9.0±0.6 (7.6-10.8)
Esophagus	358	366.6±10.6 (348.4-387.2)	289.6±28.2 (272.2-345.6)	311.4±14.7 (275.0-344.2)
Esophageal bulb length	78	81.5±2.1 (77.4-85.1)	63.7±2.2 (60.1-66.1)	70.4±3.0 (64.8-76.5)
Esophageal bulb diam.	24	24.5±1.1 (22.5-26.5)	16.5±1.4 (14.1-17.9)	18.6±0.6 (17.6-23.0)
Body diam.	42	42.7±2.0 (38.8-46.7)	27.2±1.9 (24.3-29.8)	29.6±1.5 (26.4-35.1)
Anterior genital branch length	11	10.6±1.3 (8.4-12.3)	—	—
G1%	0.5	0.5±0.1 (0.4-0.6)	—	—
Posterior genital branch length	240	218.3±40.9 (119.2-292.1)	—	—
G2%	8.9	10.7±1.9 (5.8-13.9)	—	—
Distance from anterior end to vulva	522	525.2±15.9 (493.0-548.3)	—	—
Anal body width.	24	23.1±1.5 (20.1-25.0)	15.2±1.0 (14.0-16.4)	18.7±0.7 (16.8-20.3)
Tail	106	112.2±6.3 (98.0-120.3)	98.0±6.0 (88.0-104.0)	109.0±1.4 (103.5-116.0)
Hyaline tail part	44	43.0±2.2 (39.4-47.2)	19.3±1.2 (17.9-20.7)	26.1±2.7 (20.2-32.0)
H%	41	38.5±2.4 (34.4-43.4)	19.5±2.0 (17.5-23.1))	23.8±2.9 (17.4-28.2)
Rectum	38	35.2±2.7 (30.4-40.8)	20.0±4.5 (16.4-27.6)	28.4±2.3 (23.1-30.8)

Sequences of nematode species used for the phylogenetic analyses.

Species	18S	28S D2-D3	ITS
Xiphinema abrantinum	-	AY601625	-
Xiphinema andalusiense	-	-	KX244924
Xiphinema aceri	EU477381	-	EU477385
Xiphinema adenohytherum	GU725084	GU725075	GU725063
Xiphinema baetica	KC567149	KC567168	KC567157
	KC567148	-	KC567156
Xiphinema bakeri	AY283173	KF292277	KF292281
	-	KF292278	AF511426
	-	KF292276	-
	-	AY601623	-
Xiphinema barense	-	KM199691	KM199694
	-	-	KM199693
Xiphinema basiri	-	AY601629	-
Xiphinema belmontense	-	KC567171	KC567158
Xiphinema bernardi	-	-	EU375483
Xiphinema brasiliense	AY297836	AY601616	-
	-	KP793050	-
Xiphinema cadavalense	-	-	KX244932
Xiphinema castilloi	-	KF446655	-
Xiphinema chambersi	AY283174	AY601617	HM138503
	HM138503	KU680967	HM191718
	HM191718	DQ299512	KJ934160
	KJ934157	-	KJ934157
	KJ934160	-	AF511428
	-	-	KU764410
	-	-	KU764405
	-	-	KU764406
	-	-	KU764407
	-	-	KU764408
	-	-	KU764409
	-	-	KU764411
Xiphinema cohni	-	KC567173	KC567159
Xiphinema costaricense	-	KX931057	KX931069
Xiphinema coxi	-	AY601631	-
Xiphinema coxi europaeum	KC567152	-	KC567161
	KC567153	-	-
Xiphinema cretense	-	KJ802878	KJ802895
Xiphinema dentatum	-	AY601627	-
Xiphinema diversicaudatum	EF538761	JQ780365	KF292282
	JQ780349	-	-
	JQ780348	-	-
	JQ780347	-	-
	JQ780346	-	-
Xiphinema ensiculiferum	AY297825	-	-
Xiphinema elongatum	AY297824	EF140790	AY524971
	KP407872	-	EF140789
Xiphinema gersoni	KC567154	KC567180	-
Xiphinema globosum	GU549476	GU549474	GU549475
Xiphinema granatum	-	JQ240273	-
Xiphinema hangzhouense	-	MF538772	MF538770
Xiphinema herakliense	KM586356	-	KM586353
	KM586357	-	KM586354
Xiphinema hispidum	HM921368	-	-
Xiphinema hispanum	GU725083	GU725074	GU725061
	-	-	HM821367
Xiphinema hunaniense	-	KP793049	AY579205
	-	EF188839	-
Xiphinema ifacolum	AY297826	-	-
Xiphinema index	EF207249	HM921349	HM921334
	AY687997	-	AY430175
	HM921342	-	-
Xiphinema ingens	-	-	KM893399
	-	-	KJ9566387
Xiphinema insigne	-	AY601619	AY553980
	-	-	AY563427
Xiphinema iranicum	EU477384	-	-
Xiphinema israeliae	-	KJ802886
Xiphinema italiae	HM921343	HM921351	HM921335
	FJ713154	-	HM921341
	-	-	AJ437029
Xiphinema japonica	KY131241	KY131240	KY131244
	-	KU052864	-
Xiphinema krugi	AY297827	KX931063	DQ017154
	AY297828	-	KX931070
Xiphinema longicaudatum	AY297829	-	-
Xiphinema lupini	-	HM921352	-
	-	KC567183	-
Xiphinema mazandaranense	HQ658630	-	-
Xiphinema macedonicum	EU477383	-	-
Xiphinema macroacanthum	-	HF546081	HF546078
Xiphinema naturale	-	DQ299515	-
Xiphinema nuragicum	GU725078	GU725070	GU725056
	GU725079	-	GU725057
	GU725080	-	-
	GU725081	-	-
Xiphinema parachambersi n. sp.	MG786444	MG7866445	MG786442
Xiphinema paritaliae	AY297831	-	-
Xiphinema pseudocoxi	-	-	KX244939
Xiphinema pyrenaicum	GU725085	AY601626	GU725060
	-	GU725073	-
Xiphinema radicicola	-	AY601622	-
Xiphinema savanicola	-	AY601620	-
Xiphinema setariae	-	AY601621	KX931075
	-	-	AY430179
	-	-	KX931077
Xiphinema sphaerocephalum	GU725082	GU725076	GU725062
Xiphinema surinamense	AY297833	-	-
Xiphinema turcicum	GU725086	KC567185	-
Xiphinema turdetanense	KC567155	KC567186	KC567163
Xiphinema variegatum	AY297834	-	-
Xiphinema vulgare	-	DQ299514	-
Xiphinema vuittenezi	AY552979	-	HG329722
	EF614267	-	AJ437028
Xiphinema zagrosense	JN153100	JN153101	-
Xiphinema sp.	AY297840	-	DQ364686
	EF207250	-	-
	EU477382	-	-
Xiphinema amercanum group
Xiphinema cf. americanum	AM086679	-	-
Xiphinema americanum	-	AY580056	-
Xiphinema brevicollum	-	HM163209	-
	-	AY601604	-
	-	KP793051	-
	-	AY601605	-
Xiphinema bricolensis	-	AY601594	-
	-	AY601596	-
Xiphinema californicum	-	AY601592	-
Xiphinema citricolum	-	DQ285668	-
Xiphinema diffusum	-	AY601600	-
Xiphinema duriense	-	JQ990032	-
Xiphinema floridae	-	DQ299508	-
Xiphinema georgianum	-	DQ299496	-
Xiphinema incertum	-	JQ990031	-
Xiphinema inaequale	-	HM163210	-
Xiphinema laevistriatum	-	DQ299504	-
Xiphinema lambertii	-	HM163211	-
Xiphinema opisthohysterum	-	JQ990040	-
Xiphinema pachtaicum	-	HM921393	-
	-	HM921356	-
Xiphinema pacificum	-	AY601590	-
Xiphinema parabrevicolle	-	JQ990042	-
Xiphinema parapachydemum	-	JQ990036	-
Xiphinema rivesi	-	AY210845	-
Xiphinema santos	-	AY601587	-
Xiphinema simile	AM086680	KJ802889	-
	AM086681	-	-
	JQ780350	-	-
Xiphinema tarjanense	-	DQ299511	-
Xiphinema taylori	-	AY601602	-
Xiphinema thornei	-	AY601595	-
Xiphinema utahense	-	AY601598	-
Xiphidorus yepesara yepesara	AY297838	-	-
Xiphidorus balcarceanus	AY297839	-	-
Longidorus ferrisi	AY283163	-	-
Longidorus raskii	-	-	AJ549984
Longidorus elongatus	-	-	AJ549987

eISSN:: 2640-396X
Język:: Angielski

Częstotliwość wydawania:: Volume Open
Dziedziny czasopisma:: Life Sciences, other

Kanał RSS czasopisma

Description of Xiphinema parachambersi sp. n. (Nematoda: Longidoridae) from Imported Ornamental Plants in Japan with a Key to Xiphinema Species in Group 1

Data publikacji: 17 paź 2018

Zakres stron: 369 - 386

DOI: https://doi.org/10.21307/jofnem-2018-027

Słowa kluczowe
Molecular, morphology, morphometrics, nematode, new species

© 2018 Munawar Maria et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Fig. 1

Fig. 2

Fig. 3

Fig. 4

Fig. 5

Fig. 6

Fig. 7

Fig. 8

Morphometrics data for Xiphinema parachambersi n. sp. All measurements are in µm and in the form of mean ± s.d. (range).

Sequences of nematode species used for the phylogenetic analyses.

Description of Xiphinema parachambersi sp. n. (Nematoda: Longidoridae) from Imported Ornamental Plants in Japan with a Key to Xiphinema Species in Group 1

Data publikacji: 17 paź 2018

Zakres stron: 369 - 386

DOI: https://doi.org/10.21307/jofnem-2018-027

Słowa kluczoweMolecular, morphology, morphometrics, nematode, new species

© 2018 Munawar Maria et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Fig. 1

Fig. 2

Fig. 3

Fig. 4

Fig. 5

Fig. 6

Fig. 7

Fig. 8

Morphometrics data for Xiphinema parachambersi n. sp. All measurements are in µm and in the form of mean ± s.d. (range).

Sequences of nematode species used for the phylogenetic analyses.

Słowa kluczowe
Molecular, morphology, morphometrics, nematode, new species