<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Trichinellosis is a zoonotic disease affecting mainly the temperate regions. The treatment is a challenge for the physician, and the available therapy is far from ideal. Therefore, this work aimed to evaluate the effect of heat shock protein 90 inhibitor, geldanamycin, on the adult worms and larvae of <italic>Trichinella spiralis</italic>. This research comprised an <italic>in vivo</italic> study in which <italic>T. spiralis</italic>-infected mice were treated by two different doses of geldanamycin, thereafter larval count and pathological changes were determined in the muscles. Meanwhile, the <italic>in vitro</italic> study investigated the effect of two different concentrations of geldanamycin on adult worms and larvae of <italic>T. spiralis</italic> via transmission electron microscopy. The <italic>in vivo</italic> study showed significant reduction of muscle larval counts under the effect of geldanamycin. Moreover, characteristic changes were noted as regards the parasite and the inflammatory response. The <italic>in vitro</italic> study revealed degenerative changes in the body wall of larvae and adults of <italic>T. spiralis</italic> under the influence of geldanamycin. In conclusion, heat shock protein 90 inhibitor, geldanamycin, seems to have detrimental effects on the adults and larvae of <italic>T. spiralis</italic>. It, or one of its derivatives, could be an adjuvant to anthelmintic therapy of trichinellosis, but more studies are warranted to establish its usefulness.</p></abstract>

Trichinellosis is a zoonotic disease affecting mainly the temperate regions. The treatment is a challenge for the physician, and the available therapy is far from ideal. Therefore, this work aimed to evaluate the effect of heat shock protein 90 inhibitor, geldanamycin, on the adult worms and larvae of Trichinella spiralis. This research comprised an in vivo study in which T. spiralis-infected mice were treated by two different doses of geldanamycin, thereafter larval count and pathological changes were determined in the muscles. Meanwhile, the in vitro study investigated the effect of two different concentrations of geldanamycin on adult worms and larvae of T. spiralis via transmission electron microscopy. The in vivo study showed significant reduction of muscle larval counts under the effect of geldanamycin. Moreover, characteristic changes were noted as regards the parasite and the inflammatory response. The in vitro study revealed degenerative changes in the body wall of larvae and adults of T. spiralis under the influence of geldanamycin. In conclusion, heat shock protein 90 inhibitor, geldanamycin, seems to have detrimental effects on the adults and larvae of T. spiralis. It, or one of its derivatives, could be an adjuvant to anthelmintic therapy of trichinellosis, but more studies are warranted to establish its usefulness.

Detrimental effects of geldanamycin on adults and larvae of <italic>Trichinella spiralis</italic>

Department of Medical Parasitology, Faculty of Medicine, Tanta University, Tanta, Egypt

Helminthologia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Detrimental effects of geldanamycin on adults and larvae of 

Trichinellosis is a zoonotic disease affecting mainly the temperate regions. The treatment is a challenge for the physician, and the available therapy is far...

	Control (n=7)	Group 1 (n=7)	Group 2 (n=7)
Mean ± SD	25,428 ± 1,397	15,428 ± 975	8,142 ± 1,069
IE	---------	40.4 %	67.9 %
F. test		14.526
P. value		0.001
Control & G1	Control & G2		G1 & G2
0.001	0.001		0.001

Detrimental effects of geldanamycin on adults and larvae of Trichinella spiralis

Article Category: Research Article

Data publikacji: 22 kwi 2016

Zakres stron: 126 - 132

Otrzymano: 03 gru 2015

Przyjęty: 22 lut 2016

DOI: https://doi.org/10.1515/helmin-2016-0003

© 2016 Institute of Parasitology, SAS, Košice

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Fig. 2

Muscle larval count in control and treated groups