Morphological and molecular characterization of  species from Shenzhen, China

Y. Zeng; W. Ye; Z. Zhang; H. Sun; L. Yong; Y. Huang; K. Zhao; H. Liang; J. Kerns

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Morphological and molecular characterization of Xiphinema species from Shenzhen, China

oraz

| 10 mar 2016

Tom 53 (2016): Zeszyt 1 (March 2016)

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Article Category: Research Article

Data publikacji: 10 mar 2016

Zakres stron: 62 - 75

Otrzymano: 12 maj 2015

Przyjęty: 31 sie 2015

DOI: https://doi.org/10.1515/helmin-2015-0068

Słowa kluczowe
spp., 28S rDNA, dagger nematode

© 2016 Institute of Parasitology, SAS, Košice

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Light micrographs of Xiphinema hunaniense from Blechnum orientale. A: Female entire body; B: Female anterior body; C: Reproductive system of female (in ventral view); D: Female tail (in ventral view); E: Female tail (in lateral view). Scale bars: A = 50 μm; B – E = 10 μm

Light micrographs of female Xiphinema brasiliense from Gleichenia linearis. A, B: Entire body; C: Anterior body; D: Tail in ventral view; E: Tail in lateral view; F: Reproductive system. Scale bars: A = 500 μm; B, C, E = 20 μm; D = 10 μm

Light micrographs of female Xiphinema americanum s.l. from Acacia mangium. A: Entire body; B: Anterior body; C: Reproductive system; D: Vulva (in ventral view); E: Tail in lateral view; F: Tail in ventral view. Scale bars: A = 100 μm; B – F = 20 μm

The 10001st Bayesian tree inferred from Xiphinema spp. 28S D2-D3 under GTR+I+G model (-lnL=10448.2939; AIC=20916.5879; freqA=0.2519; freqC=0.2207; freqG=0.3013; freqT=0.2261; R(a)=0.9498; R(b)=2.5514; R(c)=2.4833; R(d)=0.5243; R(e)=4.4187; R(f)=1; Pinva=0.3354; Shape=0.8037). Posterior probability values exceeding 50% are given on appropriate clades

The codes of the Chinese Xiphinema americanum s.l. populations and the close species in X. americanum-group sensu Lamberti et al. (2000)

Species/Codes	A	J	C	H	В	D	Е	F	G	I
X. americanum s.l.	2	2	2	2(3)	2	1	1(2)	1	2	2
X. taylori	2	2	2	3(2)	3(2)	1	1	1(2)	2	2(1)
X. diffusum	2	2	2(1)	2(1)	2	1	1	1	2	1(2)
X. incognitum	2	2	2(1)	2(3)	2(3)	1(2)	1	1	2(1)	2
X. brevicolle	2	2	3(2)	3	3(2)	1	2(3(1)	1	2	2(1)
X. parabrevicolle	2	2	3(2)	3	3(2)	1	2(1)	1	2	1(2)

Morphometrics of females of Xiphinema spp. populations mounted in formalin–glycerin in this study. All measurements in μm and in the format: mean ± s.d. (Range)

	X hunaniense	X hunaniense	X hunaniense	X hunaniense	X brasiliense	X americanum s.l.	X americanum s.l.	X americanum s.l.	X americanum s.l.	X americanum s.l.
	SZX1301	SZX1302	SZX1303	SZX1304	SZX1305	SZX1306	SZX1307	SZX1308	SZX1309	SZX1310
	Host	Blechnum orientate	Blechnum orientate	Gleichenia linearis	Litchi chinensis	Gleichenia linearis	Acacia mangium	Blechnum orientate Blechnum orientate	Acacia confusa	Litchi chinensis
n	10	10	10	10	5	10	10	10	10	10
L	2098.8 ±96.1 (1989.6-2200.0)	2236.4 ±176.9 (2001.8-2400.0)	2110.5 ± 100.0 (1998.7-2202.7)	2018.6 ±141.1 (1810.0-2179.3)	1643.1 ±346.8 (1108.0-2100.0)	1792.6 ±80.7 (1669.0-1888.0)	1808.6 ±86.7 (1691.0-1904.0)	1876.1 ± 144.7 (1696.0-2105.6)	1785.6 ± 116.2 (1643.8-1932.0)	1807.5 ±94.8 (1683.0-1909.0)
a	42.1 ±3.4 (39.7-48.0)	46.9 ± 4.3 (42.5-52.7)	46.8 ±3.1 (43.0-50.1)	46.2 ± 2.5 (43.0-49.1)	38.4 ±5.5 (30.0-47.9)	45.1 ± 1.0 (43.8-46.2)	45.6 ± 1.0 (44.5-47.1)	46.8 ±4.1 (38.1-50.1)	46.6 ±2.2 (44.7-50.4)	44.7 ±2.1 (41.0-46.0)
b	5.9 ±0.3 (5.6-6.4)	6.1 ±0.2 (5.9-6.5)	6.0 ±0.2 (5.9-6.3)	5.6 ±0.5 (5.0-6.1)	5.5 ± 0.3 (5.2-6.0)	6.8 ±0.4 (6.1-7.2)	6.7 ±0.6 (5.9-7.3)	6.7 ±0.6 (5.9-7.7)	6.6 ±0.4 (6.1-7.0)	6.4 ±0.7 (5.3-7.0)
c	45.9 ± 5.7 (38.0-51.6)	48.0 ±3.8 (41.7-51.0)	40.4 ±3.8 (37.1-46.8)	39.9 ±2.2 (37.6-43.0)	41.6 ±2.8 (39.0-48.1)	63.0 ±5.8 (57.4-69.4)	64.1 ±9.8 (56.2-78.7)	68.3 ±10.3 (56.3-84.9)	64.5 ±9.1 (56.1-76.7)	64.3 ± 4.0 (58.1-69.2)
c’	1.6 ± 0.1 (1.5-1.7)	1.6 ± 0.1 (1.6-1.7)	1.9 ±0.3 (1.7-2.3)	2.1 ±0.4 (1.7-2.6)	1.2 ± 0.1 (1.0-1.3)	1.1 ±0.1 (1.0-1.4)	1.1 ±0.1 (0.9-1.2)	1.1 ±0.2 (0.9-1.3)	1.1 ±0.1 (1.0-1.2)	1.1 ±0.1 (1.0-1.3)
V	27.6 ±0.4 (27.0-28.0)	26.9 ±0.8 (26.0-27.8)	26.0 ± 1.6 (23.3-27.2)	26.8 ±0.8 (26.0-28.0)	27.3 ±1.0 (26.0-29.0)	51.8 ± 1.7 (50.3-54.5)	52.4 ± 1.5 (50.9-54.7)	51.9 ± 1.1 (50.6-53.2)	52.5 ±1.6 (50.8-54.5)	52.7 ±0.9 (51.6-53.9)
Odontostyle	121.1 ±4.6 (116.8-128.6)	120.6 ±4.2 (116.9-127.7)	114.0 ±3.1 (109.0-117.2)	115.2 ±3.7 (110.3-119.0)	123.8 ±4.9 (116.0-132.0)	89.0 ± 1.0 (87.9-90.2)	93.1 ±3.0 (89.8-95.9)	94.8 ±7.5 (82.7-107.6)	89.0 ±2.4 (85.9-92.3)	92.5 ± 4.0 (88.5-97.2)
Odontophore	66.9 ±3.5 (63.8-71.3)	65.1 ±0.7 (64.2-66.2)	70.3 ±1.0 (68.9-71.4)	64.3 ± 4.9 (60.0-70.0)	69.5 ± 4.8 (65.0-80.0)	50.4 ±1.5 (48.9-52.3)	52.0 ±1.7 (49.7-53.8)	53.2 ±2.4 (49.6-58.0)	51.0 ±1.6 (48.9-53.2)	51.1 ±2.7 (48.7-55.6)
Onchiotyle	187.9 ±4.6 (181.8-192.9)	185.7 ±4.2 (182.2-192.9)	184.3 ±3.9 (177.9-188.2)	179.5 ±5.9 (172.7-189.0)	193.3 ±9.3 (182.0-212.0)	139.5 ±2.3 (136.9-142.5)	145.0 ±4.6 (139.8-149.6)	142.4 ±7.0 (132.3-150.3)	140.0 ±3.4 (136.8-145.5)	143.7 ±6.2 (137.2-152.8)
Tail	46.5 ±7.2 (40.2-55.9)	46.6 ±2.1 (44.0-49.0)	52.7 ± 6.8 (42.9-59.3)	50.8 ±5.9 (44.2-57.9)	39.7 ±8.9 (26.9-51.0)	28.6 ±2.2 (26.0-31.9)	28.7 ±3.8 (24.2-32.6)	27.8 ±3.2 (23.9-33.2)	28.0 ± 2.8 (25.1-31.5)	28.2 ± 2.5 (26.3-32.6)
Width at lip	11.0 ±0.8 (10.0-12.0)	10.9 ±0.6 (10.0-11.5)	10.5 ±1.0 (9.7-12.0)	10.5 ±0.5 (10.0-11.0)	11.2 ± 0.9 (10.0-12.6)	10.6 ±0.4 (10.0-11.0)	10.6 ±0.4 (10.0-11.0)	10.6 ±0.4 (10.0-11.2)	10.6 ±0.4 (10.0-11.0)	10.6 ±0.4 (10.0-11.0)
Width at mid. body	50.1 ±4.6 (44.3-55.0)	47.9 ± 4.8 (43.8-56.1)	45.2 ±1.3 (44.0-46.7)	43.6 ±0.9 (42.1-44.4)	43.0 ±8.7 (32.2-60.0)	39.7 ±2.4 (36.2-42.0)	39.7 ±1.3 (38.0-41.5)	40.5 ±6.0 (34.9-55.2)	38.4 ±3.7 (33.7-43.2)	40.5 ±1.5(38.9-42.9)
Width at anus	28.6 ±3.2 (25.3-32.9)	28.8 ±1.1 (27.4-30.0)	27.3 ± 3.4 (23.8-32.4)	24.8 ±3.0 (22.1-29.6)	33.8 ±8.3 (23.4-46.7)	25.2 ±0.5 (24.5-25.7)	25.4 ±1.2 (23.5-26.4)	25.4 ±1.7 (23.2-27.9)	25.6 ± 0.8 (24.6-26.5)	25.3 ±3.1 (22.3-29.5)
Pharynx	355.9 ± 25.2 (332.0-386.7)	367.3 ±21.0 (339.3-388.8)	350.1 ± 16.8 (329.3-372.4)	360.3 ± 33.8 (312.1-401.6)	301.5 ±65.2 (205.2-375.0)	264.1 ±22.6 (238.3-300.5)	273.5 ± 28.6 (239.7-304.0)	280.6 ±24.1 (242.4-322.8)	271.3 ±29.7 (241.0-304.0)	283.8 ±31.9(247.5-333.3)
Oral aperture to ring guide	101.5 ± 3.4 (98.0-106.7)	102.5 ±4.8 (97.3-108.2)	94.1 ±5.7 (85.5-100.0)	97.5 ±1.3 (95.9-99.3)	115.1 ±3.7 (109.2-120.0)	74.6 ±2.7 (72.7-79.4)	78.3 ± 4.8 (72.0-84.5)	79.7 ± 4.2 (71.6-86.9)	73.8 ± 2.6 (70.9-76.7)	74.7 ± 8.0 (69.3-88.9)

The codes of the Chinese Xiphinema americanum s.l. populations and X. incognitum and X. brevicolle sensu Lamberti et al. (2004)

Species/Codes	G	H	A	C	B	D	E	F	I
X. americanum s.l.	2	2	3(4)	2	2	2	2	1	2
X. incognitum	2	2	3	2	2	2	2	1	2
X. brevicolle	2	2	5	1	2	23	23	1	12

Species and populations of Xiphinema in this study

Species	Population code	Locality	Host plant	18S Accession No.	28S D2-D3 Accession No.
Xiphinema hunaniense	SZX1301	Tianxinshan, Shenzhen	Blechnum orientale	KP79303	KP793046
Xiphinema hunaniense	SZX1302	Tianxinshan, Shenzhen	Blechnum orientale	KP793037	KP793047
Xiphinema hunaniense	SZX1303	Yangmeikeng, Shenzhen	Gleichenia linearis	KP793038	KP793048
Xiphinema hunaniense	SZX1304	Tianxinshan, Shenzhen	Litchi chinensis	KP793039	KP793049
Xiphinema brasiliense	SZX1305	Yangmeikeng, Shenzhen	Gleichenia linearis	KP793040 .	KP793050
Xiphinema americanum s.l	. SZX1306	Tianxinshan, Shenzhen	Acacia mangium	KP793041	KP793051
Xiphinema americanum s.l	. SZX1307	Tianxinshan, Shenzhen	Blechnum orientale	KP793042	KP793052
Xiphinema americanum s.l	.SZX1308	Tianxinshan, Shenzhen	Blechnum orientale	KP793043	KP793053
Xiphinema americanum s.l	. SZX1309	Tianxinshan, Shenzhen	Acacia confuse	KP793044	KP793054
Xiphinema americanum s.l	. SZX1310	Tianxinshan, Shenzhen	Litchi chinensis	KP793045	KP793055

eISSN:: 1336-9083
ISSN:: 0440-6605
Język:: Angielski

Częstotliwość wydawania:: 4 razy w roku
Dziedziny czasopisma:: Life Sciences, Zoology, Ecology, other, Medicine, Clinical Medicine, Microbiology, Virology and Infection Epidemiology

Kanał RSS czasopisma

Morphological and molecular characterization of Xiphinema species from Shenzhen, China

Article Category: Research Article

Data publikacji: 10 mar 2016

Zakres stron: 62 - 75

Otrzymano: 12 maj 2015

Przyjęty: 31 sie 2015

DOI: https://doi.org/10.1515/helmin-2015-0068

Słowa kluczowe
spp., 28S rDNA, dagger nematode

© 2016 Institute of Parasitology, SAS, Košice

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1.

Fig. 2.

Fig. 3.

Fig. 4.

The codes of the Chinese Xiphinema americanum s.l. populations and the close species in X. americanum-group sensu Lamberti et al. (2000)

Morphometrics of females of Xiphinema spp. populations mounted in formalin–glycerin in this study. All measurements in μm and in the format: mean ± s.d. (Range)

The codes of the Chinese Xiphinema americanum s.l. populations and X. incognitum and X. brevicolle sensu Lamberti et al. (2004)

Species and populations of Xiphinema in this study

Morphological and molecular characterization of Xiphinema species from Shenzhen, China

Article Category: Research Article

Data publikacji: 10 mar 2016

Zakres stron: 62 - 75

Otrzymano: 12 maj 2015

Przyjęty: 31 sie 2015

DOI: https://doi.org/10.1515/helmin-2015-0068

Słowa kluczowespp., 28S rDNA, dagger nematode

© 2016 Institute of Parasitology, SAS, Košice

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1.

Fig. 2.

Fig. 3.

Fig. 4.

The codes of the Chinese Xiphinema americanum s.l. populations and the close species in X. americanum-group sensu Lamberti et al. (2000)

Morphometrics of females of Xiphinema spp. populations mounted in formalin–glycerin in this study. All measurements in μm and in the format: mean ± s.d. (Range)

The codes of the Chinese Xiphinema americanum s.l. populations and X. incognitum and X. brevicolle sensu Lamberti et al. (2004)

Species and populations of Xiphinema in this study

Słowa kluczowe
spp., 28S rDNA, dagger nematode