<abstract xmlns="http://www.w3.org/1999/xhtml"><p> In horses, muscle biopsy is widely used in veterinary practice for routine diagnosis of various muscle disorders. Development of genetic methods such as gene expression measurements using microarrays, RNA-seq, and real-time PCR leads to searching an alternative, less invasive biopsy method in order to obtain an appropriately representative group of animals for genetic testing. In the present study, we proposed a minimally invasive method of muscle sample biopsy using Pro- MagTM Ultra Automatic Biopsy Instrument (Surgical Specialties Corporation, US, Inc.), which is commonly utilized in human medicine. This approach does not require skin incisions and usage of stitches. The quantity of muscle sample gained in accordance with presented procedure was sufficient to obtain RNA with a good quality. Furthermore, application of the automatic biopsy instrument allows obtaining a sufficient amount of biological material for genetic analysis from a wide variety of tissues. Moreover, samples acquired according to this method could be used in other analyses.</p></abstract>

In horses, muscle biopsy is widely used in veterinary practice for routine diagnosis of various muscle disorders. Development of genetic methods such as gene expression measurements using microarrays, RNA-seq, and real-time PCR leads to searching an alternative, less invasive biopsy method in order to obtain an appropriately representative group of animals for genetic testing. In the present study, we proposed a minimally invasive method of muscle sample biopsy using Pro- MagTM Ultra Automatic Biopsy Instrument (Surgical Specialties Corporation, US, Inc.), which is commonly utilized in human medicine. This approach does not require skin incisions and usage of stitches. The quantity of muscle sample gained in accordance with presented procedure was sufficient to obtain RNA with a good quality. Furthermore, application of the automatic biopsy instrument allows obtaining a sufficient amount of biological material for genetic analysis from a wide variety of tissues. Moreover, samples acquired according to this method could be used in other analyses.

Evaluation of minimally invasive muscle biopsy method for genetic analysis in horse

Institute of Animal Science, Department of Horse Breeding, University of Agriculture in Krakow, Al. Mickiewicza 24-28, 30-059 Kraków, Poland

Laboratory of Genomics, National Research Institute of Animal Production, 32-083 Balice n. Kraków, Poland

Department of Animal Genetics and Breeding, National Research Institute of Animal Production, 32-083 Balice n. Kraków, Poland

Veterinary Office, Wet 24, Buczków 91, 32-712 Bratucice, Poland

Veterinary Clinic Red-Vet, Gorysławice 118, 28-160 Wiślica, Poland

Annals of Animal Science

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

In horses, muscle biopsy is widely used in veterinary practice for routine diagnosis of various muscle disorders. Development of genetic methods such as gene...

Evaluation of minimally invasive muscle biopsy method for genetic analysis in horse

Article Category: Animal genetics and breeding

Data publikacji: 29 lip 2015

Zakres stron: 621 - 627

Otrzymano: 14 paź 2014

Przyjęty: 16 sty 2015

DOI: https://doi.org/10.1515/aoas-2015-0017

Słowa kluczowe
muscle biopsy, horse, automatic biopsy, genetic analysis

© by Monika Stefaniuk

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Evaluation of minimally invasive muscle biopsy method for genetic analysis in horse

Article Category: Animal genetics and breeding

Data publikacji: 29 lip 2015

Zakres stron: 621 - 627

Otrzymano: 14 paź 2014

Przyjęty: 16 sty 2015

DOI: https://doi.org/10.1515/aoas-2015-0017

Słowa kluczowemuscle biopsy, horse, automatic biopsy, genetic analysis

© by Monika Stefaniuk

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Słowa kluczowe
muscle biopsy, horse, automatic biopsy, genetic analysis