Rivista e Edizione

Volume 7 (2023): Edizione 1 (January 2023)

Volume 6 (2022): Edizione 4 (October 2022)

Volume 6 (2022): Edizione 3 (July 2022)

Volume 6 (2022): Edizione 2 (April 2022)

Volume 6 (2022): Edizione 1 (January 2022)

Volume 5 (2021): Edizione 4 (October 2021)

Volume 5 (2021): Edizione 3 (July 2021)

Volume 5 (2021): Edizione s2 (December 2021)

Volume 5 (2021): Edizione 2 (April 2021)

Volume 5 (2021): Edizione 1 (January 2021)

Volume 5 (2021): Edizione s1 (June 2021)

Volume 4 (2020): Edizione 4 (October 2020)

Volume 4 (2020): Edizione 3 (July 2020)

Volume 4 (2020): Edizione 2 (April 2020)

Volume 4 (2020): Edizione 1 (January 2020)

Volume 3 (2019): Edizione 4 (October 2019)

Volume 3 (2019): Edizione 3 (July 2019)

Volume 3 (2019): Edizione 2 (April 2019)

Volume 3 (2019): Edizione 1 (January 2019)

Volume 2 (2018): Edizione 4 (October 2018)

Volume 2 (2018): Edizione 3 (July 2018)

Volume 2 (2018): Edizione 2 (April 2018)

Volume 2 (2018): Edizione 1 (January 2018)

Volume 2 (2018): Edizione s1 (September 2018)

Volume 1 (2017): Edizione 4 (October 2017)

Volume 1 (2017): Edizione 3 (July 2017)

Volume 1 (2017): Edizione 2 (May 2017)

Volume 1 (2017): Edizione s2 (December 2017)
MAGI group activity - Research, diagnosis and treatment of genetic and rare diseases

Volume 1 (2017): Edizione 1 (January 2017)

Volume 1 (2017): Edizione s1 (October 2017)
EBTNA Utility Gene Test on Ophthalmology

Dettagli della rivista
Formato
Rivista
eISSN
2564-615X
Pubblicato per la prima volta
30 Jan 2017
Periodo di pubblicazione
4 volte all'anno
Lingue
Inglese

Cerca

Volume 6 (2022): Edizione 3 (July 2022)

Dettagli della rivista
Formato
Rivista
eISSN
2564-615X
Pubblicato per la prima volta
30 Jan 2017
Periodo di pubblicazione
4 volte all'anno
Lingue
Inglese

Cerca

5 Articoli
Accesso libero

Capsanthin induces death in human prostate cancer cell lines by inducing DNA damage

Pubblicato online: 18 Jul 2022
Pagine: 99 - 104

Astratto

Abstract

There is a relationship between a person’s diet and the development and prevention of some cancers. Carotenoids are found as various natural pigments in many fruits and vegetables. Studies on carotenoids and their potential roles in carcinogenesis are increasing in importance day by day. In this study, we aimed to determine the cytotoxic and genotoxic effects of capsanthin, a carotenoid compound, in human prostate cancer cell lines.

After different concentrations of capsanthin were applied to human prostate cancer cell lines (LNCaP and PC-3), the effects of the compound on cell viability were determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test. The single-cell gel electrophoresis (Comet) assay was then used to reveal the genotoxic effects of probable cytotoxic dosages on cell DNA. After the treatments, apoptotic cell death levels were determined by Tunel staining. At high concentrations, capsanthin dramatically reduced PC-3 and LNCaP cell viability (p<0.05). In addition, capsanthin caused DNA damage and apoptotic cell death in the prostate cancer cells. The results show that capsanthin reduces cell viability by causing genotoxicity in prostate cancer cells.

Parole chiave

  • Capsanthin
  • Prostate cancer
  • Cell Culture
  • DNA damage
  • Cell Viability
Accesso libero

Corchorus olitorius L. (Jute) leaf and seed extracts exerted high antibacterial activity against food and plant pathogenic bacteria

Pubblicato online: 18 Jul 2022
Pagine: 105 - 115

Astratto

Abstract

Aim of this study was to comparatively evaluate antibacterial activities of methanol (MetOH), acetone (Ace), petroleum ether (PE) and aqueous (dw) leaf (L), root (R), and seed (S) extracts of Corchorus olitorius L. on both food- and plant-borne pathogens, with DPPH radical scavenging activities (DRSA), and quantitative and qualitative constituent analysis. Leaf PE has the highest strain susceptibility on both food- and plant-borne pathogens. Clavibacter michiganensis, Pseudomonas tomato, and Erwinia caratovora were susceptible to nearly all the leaf and seed extracts. Very low minimum inhibitory concentration (8-128 μg mL−1) and minimum bactericidal concentration (32-2048 μg mL−1) were determined for both leaf and seed extracts against C. michiganensis. Total phenolic contents were correlated to DRSA. The phenolic compounds tested were higher in the leaf MetOH, cholorogenic acid being the most abundant one. Palmitic acid was determined in leaf PE and seed PE extracts. Results presented here demonstrate high antibacterial activity of C. olitorius leaf seed extracts against phytopathogens for the first time, and provide the most comprehensive data on the antibacterial activity screening against food-borne pathogens. Considering limitations in plant disease control, antibacterial activities of these extracts would be important in plant disease control.

Parole chiave

  • Jute
  • antibacterial activity
  • food-borne pathogens
  • phytopathogens
  • phenolic contents
  • fatty acid composition
Accesso libero

Development of a database of RNA helicase inhibitors (VHIMDB) of pathogenic viruses and in silico screening for the potential drug molecules

Pubblicato online: 18 Jul 2022
Pagine: 116 - 125

Astratto

Abstract

The pathogenic RNA virus that infects human beings contains the RNA helicase enzyme, responsible for the replication of the viral genome. The enzyme is used as a suitable target against which the drug molecule acts. Therefore, the identification and proposal the novel compounds that can be targeted toward the helicase enzymes to stop the functioning of the enzyme is desirable. Although many viral helicase inhibitor molecules have been identified, still yet no unique database is available for these compounds. This research work envisages developing a curated database of RNA helicase inhibitors. The database contains in total of 353 entries that are computationally predicted and experimentally verified RNA helicase inhibitors. The database contains information like compound name, chemical properties, chemical format, and name of the target virus to which it acts against it with a user-friendly menu-driven search engine. Presently, the database is freely available at: https://vhimdb.rsatpathy.in/. Further, in silico screening of the whole database by drug-likeness and toxicity resulted in 14 potential drug molecules. The selected molecules were analyzed for their effectiveness in binding by using molecular docking score and interaction with the helicase enzymes of three categories of pathogenic viruses (SARS-CoV-2, SARS-CoV, and MERS-CoV).

Parole chiave

  • relational database
  • RNA helicase inhibitor
  • pathogenic virus
  • web development
  • molecular docking
  • interaction analysis
  • drug development
Accesso libero

Association of the 5HTR2C gene Ser23 variation with childhood allergic asthma

Pubblicato online: 18 Jul 2022
Pagine: 126 - 132

Astratto

Abstract

Objective: Allergic asthma is the most frequently observed subtype of individuals with asthma. The effects of serotonin plays in the pathophysiology of asthma has not been clearly determined. Thus, this study aimed to investigate the association between the 5HTR2C gene rs 6318 G>C polymorphism and allergic asthma in pediatric patients in Cyprus.

Methods: This study included total number of 177 individuals with 118 control and 59 pediatric patients (43 atopic and 16 non-atopic asthma patient). A skin prick test was performed for each patient to confirm asthma diagnosis and to evaluate atopic status. Genotyping for the 5HTR2C was completed by Real Time-PCR analysis.

Results: The genotype distribution frequencies were not in agreement with the Hardy-Weinberg Equilibrium in the patients’ group (p<0.00001). The frequency of the risk allele (allele C) was not significantly different between the patient and control groups (p=0.255). The genotypic distribution between atopic asthma and non-atopic asthma within the patientsts groups was not in agreement with the Hardy-Weinberg Equilibrium (p=0.006). However, risk allele presence showed a statistically significant association with atopy-related asthma (p=0.037).

Discussion & Conclusion: Overall, despite the finding of no association between the 5HTR2C rs6318 C allele and childhood asthma, the current results indicated that there is a strong association between the 5HTR2C rs6318 C variant and childhood atopic asthma.

Parole chiave

  • Childhood Allergic Asthma
  • Atopy
  • rs6318
Accesso libero

A Comprehensive Assessment of The Eight Vital Signs

Pubblicato online: 18 Jul 2022
Pagine: 133 - 146

Astratto

Abstract

The term “vital sign” has been assigned to various phenomena with the presumptive intent to emphasize their importance in health care resulting in the emergence of eight vital signs with multiple designations and overlapping terms. This review developed a case definition for vital signs and identified and described the fifth through eighth vital signs. PubMed/Medline, Google and biographical databases were searched using the individual Medical Subject Headings (MeSH) terms, vital sign and fifth, vital sign and sixth, vital sign and seventh, and vital sign eighth. The search was limited to human clinical studies written in English literature from 1957 up until November 30, 2021. Excluded were articles containing the term vital sign if used alone without the qualifier fifth, sixth, seventh, or eighth or about temperature, blood pressure, pulse, and respiratory rate. One hundred ninety-six articles (122 for the fifth vital sign, 71 for the sixth vital sign, two for the seventh vital sign, and one for the eighth vital sign) constituted the final dataset. The vital signs consisted of 35 terms, classified into 17 categories compromising 186 unique papers for each primary authored article with redundant numbered vital signs for glucose, weight, body mass index, and medication compliance. Eleven terms have been named the fifth vital sign, 25 the sixth vital sign, three the seventh, and one as the eighth vital sign. There are four time-honored vital signs based on the case definition, and they represent an objective bedside measurement obtained noninvasively that is essential for life. Based on this case definition, pulse oximetry qualifies as the fifth while end-tidal CO2 and cardiac output as the sixth. Thus, these terms have been misappropriated 31 times. Although important to emphasize in patient care, the remainder are not vital signs and should not be construed in this manner.

Parole chiave

  • pain
  • pulse oximetry
  • physical examination
  • vital signs
  • measurements
5 Articoli
Accesso libero

Capsanthin induces death in human prostate cancer cell lines by inducing DNA damage

Pubblicato online: 18 Jul 2022
Pagine: 99 - 104

Astratto

Abstract

There is a relationship between a person’s diet and the development and prevention of some cancers. Carotenoids are found as various natural pigments in many fruits and vegetables. Studies on carotenoids and their potential roles in carcinogenesis are increasing in importance day by day. In this study, we aimed to determine the cytotoxic and genotoxic effects of capsanthin, a carotenoid compound, in human prostate cancer cell lines.

After different concentrations of capsanthin were applied to human prostate cancer cell lines (LNCaP and PC-3), the effects of the compound on cell viability were determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test. The single-cell gel electrophoresis (Comet) assay was then used to reveal the genotoxic effects of probable cytotoxic dosages on cell DNA. After the treatments, apoptotic cell death levels were determined by Tunel staining. At high concentrations, capsanthin dramatically reduced PC-3 and LNCaP cell viability (p<0.05). In addition, capsanthin caused DNA damage and apoptotic cell death in the prostate cancer cells. The results show that capsanthin reduces cell viability by causing genotoxicity in prostate cancer cells.

Parole chiave

  • Capsanthin
  • Prostate cancer
  • Cell Culture
  • DNA damage
  • Cell Viability
Accesso libero

Corchorus olitorius L. (Jute) leaf and seed extracts exerted high antibacterial activity against food and plant pathogenic bacteria

Pubblicato online: 18 Jul 2022
Pagine: 105 - 115

Astratto

Abstract

Aim of this study was to comparatively evaluate antibacterial activities of methanol (MetOH), acetone (Ace), petroleum ether (PE) and aqueous (dw) leaf (L), root (R), and seed (S) extracts of Corchorus olitorius L. on both food- and plant-borne pathogens, with DPPH radical scavenging activities (DRSA), and quantitative and qualitative constituent analysis. Leaf PE has the highest strain susceptibility on both food- and plant-borne pathogens. Clavibacter michiganensis, Pseudomonas tomato, and Erwinia caratovora were susceptible to nearly all the leaf and seed extracts. Very low minimum inhibitory concentration (8-128 μg mL−1) and minimum bactericidal concentration (32-2048 μg mL−1) were determined for both leaf and seed extracts against C. michiganensis. Total phenolic contents were correlated to DRSA. The phenolic compounds tested were higher in the leaf MetOH, cholorogenic acid being the most abundant one. Palmitic acid was determined in leaf PE and seed PE extracts. Results presented here demonstrate high antibacterial activity of C. olitorius leaf seed extracts against phytopathogens for the first time, and provide the most comprehensive data on the antibacterial activity screening against food-borne pathogens. Considering limitations in plant disease control, antibacterial activities of these extracts would be important in plant disease control.

Parole chiave

  • Jute
  • antibacterial activity
  • food-borne pathogens
  • phytopathogens
  • phenolic contents
  • fatty acid composition
Accesso libero

Development of a database of RNA helicase inhibitors (VHIMDB) of pathogenic viruses and in silico screening for the potential drug molecules

Pubblicato online: 18 Jul 2022
Pagine: 116 - 125

Astratto

Abstract

The pathogenic RNA virus that infects human beings contains the RNA helicase enzyme, responsible for the replication of the viral genome. The enzyme is used as a suitable target against which the drug molecule acts. Therefore, the identification and proposal the novel compounds that can be targeted toward the helicase enzymes to stop the functioning of the enzyme is desirable. Although many viral helicase inhibitor molecules have been identified, still yet no unique database is available for these compounds. This research work envisages developing a curated database of RNA helicase inhibitors. The database contains in total of 353 entries that are computationally predicted and experimentally verified RNA helicase inhibitors. The database contains information like compound name, chemical properties, chemical format, and name of the target virus to which it acts against it with a user-friendly menu-driven search engine. Presently, the database is freely available at: https://vhimdb.rsatpathy.in/. Further, in silico screening of the whole database by drug-likeness and toxicity resulted in 14 potential drug molecules. The selected molecules were analyzed for their effectiveness in binding by using molecular docking score and interaction with the helicase enzymes of three categories of pathogenic viruses (SARS-CoV-2, SARS-CoV, and MERS-CoV).

Parole chiave

  • relational database
  • RNA helicase inhibitor
  • pathogenic virus
  • web development
  • molecular docking
  • interaction analysis
  • drug development
Accesso libero

Association of the 5HTR2C gene Ser23 variation with childhood allergic asthma

Pubblicato online: 18 Jul 2022
Pagine: 126 - 132

Astratto

Abstract

Objective: Allergic asthma is the most frequently observed subtype of individuals with asthma. The effects of serotonin plays in the pathophysiology of asthma has not been clearly determined. Thus, this study aimed to investigate the association between the 5HTR2C gene rs 6318 G>C polymorphism and allergic asthma in pediatric patients in Cyprus.

Methods: This study included total number of 177 individuals with 118 control and 59 pediatric patients (43 atopic and 16 non-atopic asthma patient). A skin prick test was performed for each patient to confirm asthma diagnosis and to evaluate atopic status. Genotyping for the 5HTR2C was completed by Real Time-PCR analysis.

Results: The genotype distribution frequencies were not in agreement with the Hardy-Weinberg Equilibrium in the patients’ group (p<0.00001). The frequency of the risk allele (allele C) was not significantly different between the patient and control groups (p=0.255). The genotypic distribution between atopic asthma and non-atopic asthma within the patientsts groups was not in agreement with the Hardy-Weinberg Equilibrium (p=0.006). However, risk allele presence showed a statistically significant association with atopy-related asthma (p=0.037).

Discussion & Conclusion: Overall, despite the finding of no association between the 5HTR2C rs6318 C allele and childhood asthma, the current results indicated that there is a strong association between the 5HTR2C rs6318 C variant and childhood atopic asthma.

Parole chiave

  • Childhood Allergic Asthma
  • Atopy
  • rs6318
Accesso libero

A Comprehensive Assessment of The Eight Vital Signs

Pubblicato online: 18 Jul 2022
Pagine: 133 - 146

Astratto

Abstract

The term “vital sign” has been assigned to various phenomena with the presumptive intent to emphasize their importance in health care resulting in the emergence of eight vital signs with multiple designations and overlapping terms. This review developed a case definition for vital signs and identified and described the fifth through eighth vital signs. PubMed/Medline, Google and biographical databases were searched using the individual Medical Subject Headings (MeSH) terms, vital sign and fifth, vital sign and sixth, vital sign and seventh, and vital sign eighth. The search was limited to human clinical studies written in English literature from 1957 up until November 30, 2021. Excluded were articles containing the term vital sign if used alone without the qualifier fifth, sixth, seventh, or eighth or about temperature, blood pressure, pulse, and respiratory rate. One hundred ninety-six articles (122 for the fifth vital sign, 71 for the sixth vital sign, two for the seventh vital sign, and one for the eighth vital sign) constituted the final dataset. The vital signs consisted of 35 terms, classified into 17 categories compromising 186 unique papers for each primary authored article with redundant numbered vital signs for glucose, weight, body mass index, and medication compliance. Eleven terms have been named the fifth vital sign, 25 the sixth vital sign, three the seventh, and one as the eighth vital sign. There are four time-honored vital signs based on the case definition, and they represent an objective bedside measurement obtained noninvasively that is essential for life. Based on this case definition, pulse oximetry qualifies as the fifth while end-tidal CO2 and cardiac output as the sixth. Thus, these terms have been misappropriated 31 times. Although important to emphasize in patient care, the remainder are not vital signs and should not be construed in this manner.

Parole chiave

  • pain
  • pulse oximetry
  • physical examination
  • vital signs
  • measurements

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