Variable region | FPM age (yr) | Total | Bacteria | Archaea | |||
---|---|---|---|---|---|---|---|
reads | OTU | reads | OTU | reads | OTU | ||
V2-V3 | 5 | 10977 ± 684 | 126 ± 12 | 1267 ± 104 | 51 ± 9 | 9710 ± 562 | 75 ± 7 |
30 | 10562 ± 513 | 100 ± 11 | 1009 ± 84 | 47 ± 8 | 9553 ± 410 | 53 ± 5 | |
100 | 11172 ± 751 | 75 ± 6 | 938 ± 53 | 27 ± 5 | 10233 ± 720 | 48 ± 4 | |
V6-V8 | 5 | 18893 ± 886 | 594 ± 17 | 17826 ± 844 | 583 ± 13 | 1067 ± 89 | 11 ± 3 |
30 | 17409 ± 395 | 428 ± 15 | 16543 ± 354 | 419 ± 11 | 866 ± 68 | 9 ± 2 | |
100 | 16894 ± 806 | 319 ± 24 | 15537 ± 758 | 312 ± 20 | 1357 ± 133 | 7 ± 2 |
FPM age (yr) | Bacteria | Archaea | ||||
---|---|---|---|---|---|---|
Chao1 | Observed OTU | Shannon | Chao1 | Observed OTU | Shannon | |
5 | 634.0 ± 84.4 | 583 ± 13 | 2.49 ± 0.56 | 97.3 ± 12.2 | 75 ± 7 | 1.374 ± 0.049 |
30 | 501.6 ± 64.6 | 419 ± 11 | 2.16 ± 0.31 | 78.5 ± 6.3 | 53 ± 5 | 1.159 ± 0.068 |
100 | 435.8 ± 62.1 | 312 ± 27 | 1.93 ± 0.34 | 74.2 ± 4.7 | 48 ± 4 | 1.068 ± 0.087 |
Variable | Values for fermentation pit age (yr)
|
||
---|---|---|---|
5 | 30 | 100 | |
Moisture(%) | 35.15 ± 6.42 | 39.62 ± 1.11 | 44.97 ± 2.83 |
Humic matter (%) | 10.54 ± 1.59 | 13.82 ± 0.09 | 10.90 ± 0.52 |
Total acidity(%) | 1.54 ± 0.09 | 1.81 ± 0.05 | 1.13 ± 0.15 |
Acetic acid(mg/g) | 3.98 ± 0.36 | 3.44 ± 0.46 | 4.21 ± 0.67 |
Lactic acid(mg/g) | 94.22 ± 1.30 | 28.81 ± 7.05 | 21.33 ± 3.15 |
Butyric acid(mg/g) | 2.87 ± 0.34 | 2.36 ± 0.26 | 2.79 ± 0.38 |
Caproic acid(mg/g) | 15.15 ± 5.12 | 29.62 ± 4.19 | 45.97 ± 5.13 |
Total nitrogen(%) | 0.94 ± 0.09 | 1.41 ± 0.08 | 2.16 ± 0.11 |
NH4 +(mg/g) | 14.12 ± 1.77 | 21.02 ± 2.03 | 25.32 ± 1.66 |
Available phosphorus(mg/g) | 4.91 ± 0.52 | 9.15 ± 0.78 | 9.15 ± 0.41 |
Available calcium(mg/g) | 9.51 ± 1.02 | 8.18 ± 1.91 | 7.41 ± 0.52 |
Taxonomic group | PLFA group | Specific PLFA markers | Reference |
---|---|---|---|
Bacteria | Multiple groups | Sum of i15:0, a15:0, i16:0, i17:0, a17:0, cy17:0, cy19:0, 16:1ω7, 18:1ω7, and 17:1ω9 | ( |
Gram-positive bacteria | Branched PLFAs | Sum of i15:0, a15:0, i16:0, i17:0, and a17:0 | ( |
Gram-negative bacteria | Cyclopropyl and mono PLFAs | Sum of cy17:0, 16:1ω7, 18:1ω7, and 17:1ω9 | (Moore-Kucera and Dick 2008) |
Actinomycetes | 10Me-PLFAs | Sum of 10Me16:0, 10Me17:0, and 10Me18:0 | (Moore-Kucera and Dick 2008) |
Anaerobes | Cyclopropyl | Sum of cy17:0, cy19:0ω7, cy19:0ω9 | (Li et al. 2017) |