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Isolation and Identification of Chlamydia abortus from Aborted Ewes in Sulaimani Province, Northern Iraq

EMAN DHAHIR ARIF

EMAN DHAHIR ARIF

Department of Microbiology, College of Veterinary Medicine, University of SulaimaniKurdistan Region, Northern Iraq
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ARIF, EMAN DHAHIR
, 
NAHLA MUHAMMAD SAEED

NAHLA MUHAMMAD SAEED

Department of Microbiology, College of Veterinary Medicine, University of SulaimaniKurdistan Region, Northern Iraq
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SAEED, NAHLA MUHAMMAD
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SHWAN KAMAL RACHID

SHWAN KAMAL RACHID

Charmo UniversityKurdistan Region, Iraq
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RACHID, SHWAN KAMAL
  
28 feb 2020
Isolation and Identification of Chlamydia abortus from Aborted Ewes in Sulaimani Province, Northern Iraq's Cover Image
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Categoria dell'articolo: original-paper
Pubblicato online: 28 feb 2020
Pagine: 65 - 71
Ricevuto: 11 dic 2019
Accettato: 30 gen 2020
DOI: https://doi.org/10.33073/pjm-2020-009
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© 2020 Eman Dhahir Arif et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Map of Iraq with Sulaimani province, showing the districts, Kalar, SaidSadiq, and Chamchamal, where samplings were carried out.
Map of Iraq with Sulaimani province, showing the districts, Kalar, SaidSadiq, and Chamchamal, where samplings were carried out.

Fig. 2.

Embryonated egg showing a dead chick embryo five days after inoculation. The infected yolk sacs were thin-walled, and their blood vessels were severely congested. Yolk appeared as a right-colored liquid, and the growth of the embryo was stunted.
Embryonated egg showing a dead chick embryo five days after inoculation. The infected yolk sacs were thin-walled, and their blood vessels were severely congested. Yolk appeared as a right-colored liquid, and the growth of the embryo was stunted.

Fig. 3.

Phylogenic trees based on the ompA gene showed that the Sul/2017 chlamydia from Iraq belonged to C. abortus and revealed that Sul/2017 has a common ancestor, respectively. The partial ompA gene of Sul/2017 has been compared with 75 sequences of Chlamydia species that were published in GenBank and MLST websites for Chlamydiales (http://pubmlst.org/chlamydiales/). The tree shows that Sul/2017 has a common ancestor with isolates of sheep in Iraq and Tunisia with accession numbers KY399850 and HQ62243 and with Sul/2014, CAAB7, H and Krauss-15 isolates that were in a group 2 of Chlamydia abortus.
Phylogenic trees based on the ompA gene showed that the Sul/2017 chlamydia from Iraq belonged to C. abortus and revealed that Sul/2017 has a common ancestor, respectively. The partial ompA gene of Sul/2017 has been compared with 75 sequences of Chlamydia species that were published in GenBank and MLST websites for Chlamydiales (http://pubmlst.org/chlamydiales/). The tree shows that Sul/2017 has a common ancestor with isolates of sheep in Iraq and Tunisia with accession numbers KY399850 and HQ62243 and with Sul/2014, CAAB7, H and Krauss-15 isolates that were in a group 2 of Chlamydia abortus.

Detection of Chlamydia abortus and Brucella abortus in different herds of sheep from three districts in Sulaimani province by PCR_

Name of district Number of samples collected Number positive for C. abortus (%) Number positive for B. abortus (%)
Kalar 15 1 (6.66) 14 (93.33)
Said Sadiq 10 0 10 (100)
Chamchamal 5 0 5(100)
Total 30 1 (3.33) 29 (96.66)

The targeted genes and PCR primers used for the detection of Chlamydia abortus and Brucella abortus in aborted ewes_

Target gene Primer name Primer sequence (5′–3′) Amplified fragment length (bp) Reference
ompA omp-F ATGAAAAAACTCTTGAAATCGG 1058 Arshi et al. 2011
omp-R CAAGATTTTCTAGACTTCATTTTGTT
bcsp31 B4-F TGGCTCGGTTGCCAATATCAA 223 Baily et al. 1992
B5-R CGCGCTTGCCTTTCAGGTCTG
Lingua:
Inglese
Frequenza di pubblicazione:
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Argomenti della rivista:
Scienze biologiche, Microbiologia e virologia
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