Accesso libero

Antibacterial Activity and Mode of Action of β-caryophyllene on Bacillus cereus

CHEW-LI MOO

CHEW-LI MOO

Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra MalaysiaSerdang, Malaysia
Cerca questo autore su
Sciendo|Google Scholar
MOO, CHEW-LI
, 
SHUN-KAI YANG

SHUN-KAI YANG

Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra MalaysiaSerdang, Malaysia
Cerca questo autore su
Sciendo|Google Scholar
YANG, SHUN-KAI
, 
MOHD-AZURAIDI OSMAN

MOHD-AZURAIDI OSMAN

Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra MalaysiaSerdang, Malaysia
Cerca questo autore su
Sciendo|Google Scholar
OSMAN, MOHD-AZURAIDI
, 
MOHD HAFIS YUSWAN

MOHD HAFIS YUSWAN

Halal Products Research Institute, University Putra MalaysiaSerdang, Malaysia
Cerca questo autore su
Sciendo|Google Scholar
YUSWAN, MOHD HAFIS
, 
JIUN-YAN LOH

JIUN-YAN LOH

Faculty of Applied Sciences, UCSI UniversityKuala Lumpur, Malaysia
Cerca questo autore su
Sciendo|Google Scholar
LOH, JIUN-YAN
, 
WEI-MENG LIM

WEI-MENG LIM

Department of Pharmaceutical Technology, School of Pharmacy, International Medical UniversityKuala Lumpur, Malaysia
Cerca questo autore su
Sciendo|Google Scholar
LIM, WEI-MENG
, 
SWEE-HUA-ERIN LIM

SWEE-HUA-ERIN LIM

Health Sciences Division, Abu Dhabi Women’s College, Higher Colleges of TechnologyAbu Dhabi, United Arab Emirates
Cerca questo autore su
Sciendo|Google Scholar
LIM, SWEE-HUA-ERIN
 e 
KOK-SONG LAI

KOK-SONG LAI

Health Sciences Division, Abu Dhabi Women’s College, Higher Colleges of TechnologyAbu Dhabi, United Arab Emirates
Cerca questo autore su
Sciendo|Google Scholar
LAI, KOK-SONG
  
12 mar 2020
Antibacterial Activity and Mode of Action of β-caryophyllene on Bacillus cereus's Cover Image
INFORMAZIONI SU QUESTO ARTICOLO
Articolo precedente
Articolo Successivo
Cita
Scarica la copertina
Categoria dell'articolo: original-paper
Pubblicato online: 12 mar 2020
Pagine: 49 - 54
Ricevuto: 19 ott 2019
Accettato: 18 gen 2020
DOI: https://doi.org/10.33073/pjm-2020-007
Parole chiave
© 2020 Chew-Li Moo et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Time-kill analysis of B. cereus treated with BCP. At MIC 2.5% BCP (v/v), the bacteria were killed within 2 hours. Cells treated at half MIC showed a suppressed and slower growth rate than the untreated cells.
Time-kill analysis of B. cereus treated with BCP. At MIC 2.5% BCP (v/v), the bacteria were killed within 2 hours. Cells treated at half MIC showed a suppressed and slower growth rate than the untreated cells.

Fig. 2.

Assays performed to evaluate the effects of BCP on the membrane surface charge and integrity of B. cereus. (A) Zeta-potential value (mV) of untreated (–5.460 mV) and BCP-treated cells (–3.980 mV); (B) Nucleic acid content of untreated and 1.25% (v/v) BCP-treated B. cereus in the extracellular environment; and (C) Protein content of untreated and 1.25% (v/v) BCP-treated B. cereus in the extracellular environment. Results were presented in mean ± SD for triplicates and considered as significant when * p < 0.050.
Assays performed to evaluate the effects of BCP on the membrane surface charge and integrity of B. cereus. (A) Zeta-potential value (mV) of untreated (–5.460 mV) and BCP-treated cells (–3.980 mV); (B) Nucleic acid content of untreated and 1.25% (v/v) BCP-treated B. cereus in the extracellular environment; and (C) Protein content of untreated and 1.25% (v/v) BCP-treated B. cereus in the extracellular environment. Results were presented in mean ± SD for triplicates and considered as significant when * p < 0.050.

Fig. 3.

The influx and efflux of EtBr in B. cereus (A) EtBr influx assay with untreated and BCP-treated cells exposed to 1 mg/l of EtBr for 60 minutes and (B) EtBr efflux assay with the cells treated with glucose or BCP only and in a combination of glucose and BCP.
The influx and efflux of EtBr in B. cereus (A) EtBr influx assay with untreated and BCP-treated cells exposed to 1 mg/l of EtBr for 60 minutes and (B) EtBr efflux assay with the cells treated with glucose or BCP only and in a combination of glucose and BCP.
Lingua:
Inglese
Frequenza di pubblicazione:
4 volte all'anno
Argomenti della rivista:
Scienze biologiche, Microbiologia e virologia
Feed RSS della rivista