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Fig. 1.
Time-kill analysis of B. cereus treated with BCP. At MIC 2.5% BCP (v/v), the bacteria were killed within 2 hours. Cells treated at half MIC showed a suppressed and slower growth rate than the untreated cells.
Fig. 2.
Assays performed to evaluate the effects of BCP on the membrane surface charge and integrity of B. cereus. (A) Zeta-potential value (mV) of untreated (–5.460 mV) and BCP-treated cells (–3.980 mV); (B) Nucleic acid content of untreated and 1.25% (v/v) BCP-treated B. cereus in the extracellular environment; and (C) Protein content of untreated and 1.25% (v/v) BCP-treated B. cereus in the extracellular environment. Results were presented in mean ± SD for triplicates and considered as significant when * p < 0.050.
Fig. 3.
The influx and efflux of EtBr in B. cereus (A) EtBr influx assay with untreated and BCP-treated cells exposed to 1 mg/l of EtBr for 60 minutes and (B) EtBr efflux assay with the cells treated with glucose or BCP only and in a combination of glucose and BCP.