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Antibacterial Activity and Mode of Action of β-caryophyllene on Bacillus cereus

CHEW-LI MOO

CHEW-LI MOO

Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra MalaysiaSerdang, Malaysia
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MOO, CHEW-LI
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SHUN-KAI YANG

SHUN-KAI YANG

Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra MalaysiaSerdang, Malaysia
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YANG, SHUN-KAI
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MOHD-AZURAIDI OSMAN

MOHD-AZURAIDI OSMAN

Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra MalaysiaSerdang, Malaysia
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OSMAN, MOHD-AZURAIDI
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MOHD HAFIS YUSWAN

MOHD HAFIS YUSWAN

Halal Products Research Institute, University Putra MalaysiaSerdang, Malaysia
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YUSWAN, MOHD HAFIS
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JIUN-YAN LOH

JIUN-YAN LOH

Faculty of Applied Sciences, UCSI UniversityKuala Lumpur, Malaysia
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LOH, JIUN-YAN
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WEI-MENG LIM

WEI-MENG LIM

Department of Pharmaceutical Technology, School of Pharmacy, International Medical UniversityKuala Lumpur, Malaysia
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LIM, WEI-MENG
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SWEE-HUA-ERIN LIM

SWEE-HUA-ERIN LIM

Health Sciences Division, Abu Dhabi Women’s College, Higher Colleges of TechnologyAbu Dhabi, United Arab Emirates
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LIM, SWEE-HUA-ERIN
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KOK-SONG LAI

KOK-SONG LAI

Health Sciences Division, Abu Dhabi Women’s College, Higher Colleges of TechnologyAbu Dhabi, United Arab Emirates
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LAI, KOK-SONG
  
12 mar 2020
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Categoría del artículo: original-paper
Publicado en línea: 12 mar 2020
Páginas: 49 - 54
Recibido: 19 oct 2019
Aceptado: 18 ene 2020
DOI: https://doi.org/10.33073/pjm-2020-007
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© 2020 Chew-Li Moo et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Time-kill analysis of B. cereus treated with BCP. At MIC 2.5% BCP (v/v), the bacteria were killed within 2 hours. Cells treated at half MIC showed a suppressed and slower growth rate than the untreated cells.
Time-kill analysis of B. cereus treated with BCP. At MIC 2.5% BCP (v/v), the bacteria were killed within 2 hours. Cells treated at half MIC showed a suppressed and slower growth rate than the untreated cells.

Fig. 2.

Assays performed to evaluate the effects of BCP on the membrane surface charge and integrity of B. cereus. (A) Zeta-potential value (mV) of untreated (–5.460 mV) and BCP-treated cells (–3.980 mV); (B) Nucleic acid content of untreated and 1.25% (v/v) BCP-treated B. cereus in the extracellular environment; and (C) Protein content of untreated and 1.25% (v/v) BCP-treated B. cereus in the extracellular environment. Results were presented in mean ± SD for triplicates and considered as significant when * p < 0.050.
Assays performed to evaluate the effects of BCP on the membrane surface charge and integrity of B. cereus. (A) Zeta-potential value (mV) of untreated (–5.460 mV) and BCP-treated cells (–3.980 mV); (B) Nucleic acid content of untreated and 1.25% (v/v) BCP-treated B. cereus in the extracellular environment; and (C) Protein content of untreated and 1.25% (v/v) BCP-treated B. cereus in the extracellular environment. Results were presented in mean ± SD for triplicates and considered as significant when * p < 0.050.

Fig. 3.

The influx and efflux of EtBr in B. cereus (A) EtBr influx assay with untreated and BCP-treated cells exposed to 1 mg/l of EtBr for 60 minutes and (B) EtBr efflux assay with the cells treated with glucose or BCP only and in a combination of glucose and BCP.
The influx and efflux of EtBr in B. cereus (A) EtBr influx assay with untreated and BCP-treated cells exposed to 1 mg/l of EtBr for 60 minutes and (B) EtBr efflux assay with the cells treated with glucose or BCP only and in a combination of glucose and BCP.
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Ciencias de la vida, Microbiología y virología
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