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Genetic Relationships Between Commercially Produced and Natural Populations of Bombus Terrestris Dalmatinus in Terms of Mitochondrial COI and CytB

Kemal Karabağ
Kemal Karabağ
, 
Emel Tüten Sevim
Emel Tüten Sevim
 e 
Şadiye Taşbaş
Şadiye Taşbaş
   | 28 dic 2021
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Article Category: Original Article
Pubblicato online: 28 dic 2021
Pagine: 315 - 330
Ricevuto: 25 mar 2021
Accettato: 05 ott 2021
DOI: https://doi.org/10.2478/jas-2021-0025
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© 2021 Kemal Karabağ et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Fig. 1

Coordinate and altitude information of the places where bee samples collected in Antalya, Turkey.
Coordinate and altitude information of the places where bee samples collected in Antalya, Turkey.

Fig. 2

Median-joining network based on the haplotypes which are determined from the mtDNA (a) COI region and (b) CytB region of B. t. dalmatinus populations in Antalya. Branches connecting together circles represent nucleotide changes among haplotypes. Underlined numbers show SNP differences between rings. Each pattern within circles illustrates the relative frequencies of sequences from different populations. The number of individuals in each haplotype is given next to the hoop. Data merged with sequences of major haplotypes reported by Lecocq et al. (2013) as references. Demre, Kumluca and Aksu are greenhouses areas, and Geyikbayiri, Phaselis, Termessos and Bayatbademler are natural areas.
Median-joining network based on the haplotypes which are determined from the mtDNA (a) COI region and (b) CytB region of B. t. dalmatinus populations in Antalya. Branches connecting together circles represent nucleotide changes among haplotypes. Underlined numbers show SNP differences between rings. Each pattern within circles illustrates the relative frequencies of sequences from different populations. The number of individuals in each haplotype is given next to the hoop. Data merged with sequences of major haplotypes reported by Lecocq et al. (2013) as references. Demre, Kumluca and Aksu are greenhouses areas, and Geyikbayiri, Phaselis, Termessos and Bayatbademler are natural areas.

AMOVA (Analyses of Molecular Variance) results and some diversity parameters calculated using COI fragments for all bumblebee populations

PopulationsSource of variation in populationsd.f.Sum of squaresVariance componentsPercentage of variationHaplotype diversityNucleotide diversity
CompaniesCompany_1Among421.550.18919.550.009
Company_2
Company_3Within11892.1030.78080.450.582
Company_4
Company_5
GreenhousecentersDemreAmong28.6120.13423.48
Kumluca0.4930.005
AksuWithin8336.1050.43976.52
Natural areasGeyikbayiri
PhaselisAmong360.130.67947.55
TermessosWithin11082.490.74952.450.8050.012
Bayatbademler
All populations (including outgroup)Among12114.8280.36834.590.6970.010
Within303211.0320.69665.41

Primer sets used in PCR amplifications of mtDNA COI and CytB gen regions of B. t. dalmatinus in Antalya

Primers (5′-3′)Gene/sizeReferences
F-CAACATTTATTTTGATTTTTTGGR-TCCAATGCACTAATCTGCCATATACOI/758 bpSperling and Hickey (1994)
F-TACTACCATGAGGACAAATATCR-ATTACACCTCCTAATTTATTGCytB/434 bpMorath (2007)

Pairwise FST values calculated for COI region below diagonal and for CytB above diagonal of the B. t. dalmatinus populations in Antalya

12345678910111213
1−0.48−0.80−0.250.00−0.24−0.91−0.85−0.25−0.03−0.130.02−0.19
2−0.850.07*0.22**0.26**0.22**0.23**0.22**0.25**0.40**0.37**0.27**0.33**
3−0.300.23**0.21**0.10*0.17**0.08**0.08**0.23**0.32**0.27**0.27**0.27**
4−0.630.08*0.16**0.37**0.15**0.34**0.36**0.040.28**0.27**0.26**0.18**
5−0.63−0.010.30**0.15**0.37**0.060.070.38**0.49**0.43**0.50**0.41**
6−0.040.060.26**0.14*0.13*0.34**0.35**0.15**0.21**0.18**0.24**0.20**
7−0.240.18**0.23**0.050.26**0.22**0.060.34**0.45**0.39**0.46**0.37**
8−0.800.010.26**0.13**0.020.13**0.23**0.37**0.48**0.41**0.48**0.39**
9−0.890.000.28**0.17**0.000.27**0.27**0.030.28**0.26**0.27**0.15**
10−0.750.050.19**0.12*0.10*0.16**0.17**0.05*0.080.020.27**0.14*
110.720.57**0.26**0.53**0.74**0.64**0.56**0.62**0.78**0.47**0.31**0.12**
12−0.030.34**−0.000.23**0.41**0.37**0.30**0.37**0.40**0.30**0.39**0.27**
130.45*0.55**0.40**0.54**0.67**0.67**0.59**0.58**0.67**0.48**0.74**0.48**

AMOVA (Analyses of Molecular Variance) results and some diversity parameters calculated using CytB fragments for all bumblebee populations

PopulationsSource of variation in populationsd.f.Sum of squaresVariance componentsPercentage of variationHaplotype diversityNucleotide diversity
CompaniesCompany_1Among495.8890.73021.670.4390.017
Company_2
Company_3141
Company_4Within372.4672.64178.33
Company_5
GreenhousecentersDemreAmong260.6391.09635.650.3800.014
KumlucaWithin75148.4251.97964.35
Aksu
Natural areasGeyikbayiri PhaselisAmong3116.6241.19720.000.7990.030
Termessos BayatbademlerWithin110526.9464.79080.00
All populations (including outgroup)Among12422.4181.22727.530.6080.023
Within3281059.9403.23172.47

Distribution of haplotypes detected in the COI gene region of the B. t. dalmatinus populations in Antalya

PopulationsH1H2H3H4H5H6H7H8H9H10H11H12H13H14H15H16
OutgroupLecocq_20131
Company_117611
Company_2131131
CompaniesCompany_39113
Company_4226
Company_51414
Demre1812
Greenhouse centersKumluca18415
Aksu182
Geyikbayiri17144
Phaselis426
Natural areasTermessos1515
Bayatbademler1612
Total166321126321313161226545

Distribution of haplotypes detected in the CytB gene region of the B. t. dalmatinus populations in Antalya

PopulationH1H2H3H4H5H6H7H8H9H10H11H12H13H14H15H16H17H18H19H20
OutgroupLecocq_20131
Company_1208
Company_22433
CompaniesCompany_318111
Company_428
Company_51812
Demre202
Greenhouse centersKumluca253
Aksu168112
Geyikbayiri11143
Natural areasPhaselis15213
Termessos264
Bayatbademler14113
Total2108811221233211321314326413
eISSN:
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