Analysis of antimicrobial resistance and genetic correlations of Escherichia coli in dairy cow mastitis

Escherichia coli is a widespread environmental pathogen frequently causing dairy cow mastitis. This bacterium is particularly capable of acquiring antimicrobial resistance, which can have severe impacts on animal food safety and human health. The objective of the study was to investigate antimicrobial resistance and genetic correlations of E. coli from dairy cow mastitis cases in northern China.

Material and Methods

Forty strains of E. coli from 196 mastitis milk samples were collected, susceptibility to 13 common antibiotics and the prevalence of resistance genes were tested in these strains, and the genetic characteristics were identified by multilocus sequence typing.

Results

The results showed that most isolates were multidrug resistant (MDR) (75%), and the resistance rates to cefazolin, trimethoprim-sulfamethoxazole and ampicillin were 77.5%, 55.0%, and 52.5%, respectively. The representative genes of the isolates were aadA (62.5%) and tet(B) (60.0%). Multilocus sequence typing showed 19 different sequence types (STs) and 5 clonal complexes (CCs) in the 40 isolates, mainly represented by ST10 and CC10. The strains of the same ST or CC showed a high level of genetic relatedness, but the characteristics of their antimicrobial resistance were markedly different.

Conclusion

Most E. coli isolates in the study were MDR strains. Some strains of the same ST or CC showed diverse resistance characteristics to common antimicrobials. Therefore, E. coli from dairy cow mastitis in northern China should be investigated to elucidate its antimicrobial resistance and genotypes.

Langue:: Anglais

Périodicité:: 4 fois par an
Sujets de la revue:: Sciences de la vie, Biologie moléculaire, Microbiologie et virologie, Sciences de la vie, autres, Médecine, Médecine vétérinaire

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Analysis of antimicrobial resistance and genetic correlations of Escherichia coli in dairy cow mastitis

Ke Li

Mingyuan Hou

Lin Zhang

Mengyue Tian

Ming Yang

Li Jia

Yanyan Liang

Dongmin Zou

Ruonan Liu

Yuzhong Ma

Publié en ligne: 04 nov. 2022

Pages: 571 - 579

Reçu: 23 mars 2022

Accepté: 28 sept. 2022

DOI: https://doi.org/10.2478/jvetres-2022-0055

Mots clésmolecular characteristics, antimicrobial resistance, genetic correlation, dairy cow mastitis

© 2022 K. Li et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction

Material and Methods

Results

Conclusion

Mots clés
molecular characteristics, antimicrobial resistance, genetic correlation, dairy cow mastitis