1. bookVolume 6 (2022): Edition 3 (July 2022)
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2564-615X
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30 Jan 2017
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access type Accès libre

Association of the 5HTR2C gene Ser23 variation with childhood allergic asthma

Publié en ligne: 18 Jul 2022
Volume & Edition: Volume 6 (2022) - Edition 3 (July 2022)
Pages: 126 - 132
Détails du magazine
License
Format
Magazine
eISSN
2564-615X
Première parution
30 Jan 2017
Périodicité
4 fois par an
Langues
Anglais
Abstract

Objective: Allergic asthma is the most frequently observed subtype of individuals with asthma. The effects of serotonin plays in the pathophysiology of asthma has not been clearly determined. Thus, this study aimed to investigate the association between the 5HTR2C gene rs 6318 G>C polymorphism and allergic asthma in pediatric patients in Cyprus.

Methods: This study included total number of 177 individuals with 118 control and 59 pediatric patients (43 atopic and 16 non-atopic asthma patient). A skin prick test was performed for each patient to confirm asthma diagnosis and to evaluate atopic status. Genotyping for the 5HTR2C was completed by Real Time-PCR analysis.

Results: The genotype distribution frequencies were not in agreement with the Hardy-Weinberg Equilibrium in the patients’ group (p<0.00001). The frequency of the risk allele (allele C) was not significantly different between the patient and control groups (p=0.255). The genotypic distribution between atopic asthma and non-atopic asthma within the patientsts groups was not in agreement with the Hardy-Weinberg Equilibrium (p=0.006). However, risk allele presence showed a statistically significant association with atopy-related asthma (p=0.037).

Discussion & Conclusion: Overall, despite the finding of no association between the 5HTR2C rs6318 C allele and childhood asthma, the current results indicated that there is a strong association between the 5HTR2C rs6318 C variant and childhood atopic asthma.

Keywords

1. Li J, Hashimoto H, Meltzer HY. Association of Serotonin2c Receptor Polymorphisms With Antipsychotic Drug Response in Schizophrenia. Front Psychiatry 2019; 10: 58. Published 2019 Feb 15. DOI:10.3389/fpsyt.2019.00058638423530828307 Ouvrir le DOISearch in Google Scholar

2. Santana N, Artigas F. Laminar and Cellular Distribution of Monoamine Receptors in Rat Medial Prefrontal Cortex. Front Neuroanat. 2017;11:87. Published 2017 Sep 28. doi:10.3389/fnana.2017.00087562502829033796 Ouvrir le DOISearch in Google Scholar

3. Stamm S, Gruber SB, Rabchevsky AG, Emeson RB. The activity of the serotonin receptor 2C is regulated by alternative splicing. Hum Genet. 2017;136(9):1079-1091. DOI:10.1007/s00439-017-1826-3587358528664341 Ouvrir le DOISearch in Google Scholar

4. Heisler LK, Pronchuk N, Nonogaki K, et al. Serotonin activates the hypothalamic-pituitary-adrenal axis via serotonin 2C receptor stimulation. J Neurosci. 2007;27(26):6956-6964. doi:10.1523/JNEUROSCI.2584-06.2007667223817596444 Ouvrir le DOISearch in Google Scholar

5. Lappalainen J, Zhang L, Dean M, et al. Identification, expression, and pharmacology of a Cys23-Ser23 substitution in the human 5-HT2c receptor gene (HTR2C). Genomics. 1995;27(2):274-279. doi:10.1006/geno.1995.10427557992 Ouvrir le DOISearch in Google Scholar

6. Holmes C, Arranz MJ, Powell JF, Collier DA, Lovestone S. 5-HT2A and 5-HT2C receptor polymorphisms and psychopathology in late onset Alzheimer’s disease. Hum Mol Genet. 1998;7(9):1507-1509. doi:10.1093/hmg/7.9.15079700207 Ouvrir le DOISearch in Google Scholar

7. Holmes C, Arranz M, Collier D, Powell J, Lovestone S. Depression in Alzheimer’s disease: the effect of serotonin receptor gene variation. Am J Med Genet B Neuropsychiatr Genet. 2003;119B(1):40-43. doi:10.1002/ajmg.b.1006812707936 Ouvrir le DOISearch in Google Scholar

8. Brummett BH, Kuhn CM, Boyle SH, Babyak MA, Siegler IC, Williams RB. Cortisol responses to emotional stress in men: association with a functional polymorphism in the 5HTR2C gene. Biol Psychol. 2012;89(1):94-98. doi:10.1016/j.biopsycho.2011.09.013324575121967853 Ouvrir le DOISearch in Google Scholar

9. Brummett BH, Babyak MA, Jiang R, et al. A functional polymorphism in the 5HTR2C gene associated with stress responses also predicts incident cardiovascular events. PLoS One. 2013;8(12):e82781. Published 2013 Dec 18. doi:10.1371/journal.pone.0082781386739324386118 Ouvrir le DOISearch in Google Scholar

10. Rosenberg SL, Miller GE, Brehm JM, Celedón JC. Stress and asthma: novel insights on genetic, epigenetic, and immunologic mechanisms. J Allergy Clin Immunol. 2014;134(5):1009-1015. doi:10.1016/j.jaci.2014.07.005425239225129683 Ouvrir le DOISearch in Google Scholar

11. Potaczek DP, Harb H, Michel S, Alhamwe BA, Renz H, Tost J. Epigenetics and allergy: from basic mechanisms to clinical applications. Epigenomics 2017;9(4):539-571. DOI:10.2217/epi-2016-016228322581 Ouvrir le DOISearch in Google Scholar

12. Sánchez-García S, Cipriani F, Ricci G. Food Allergy in childhood: phenotypes, prevention and treatment. Pediatric Allergy and Immunology: Official Publication of the European Society of Pediatric Allergy and Immunology 2015 Dec;26(8):711-720. DOI: 10.1111/pai.12514.26595763 Ouvrir le DOISearch in Google Scholar

13. Dave ND, Xiang L, Rehm KE, Marshall GD Jr. Stress and allergic diseases. Immunol Allergy Clin North Am 2011;31(1):55-68. DOI:10.1016/j.iac.2010.09.009326404821094923 Ouvrir le DOISearch in Google Scholar

14. Barnthouse M, Jones BL. The Impact of Environmental Chronic and Toxic Stress on Asthma. Clin Rev Allergy Immunol. 2019;57(3):427-438. doi:10.1007/s12016-019-08736-x31079340 Ouvrir le DOISearch in Google Scholar

15. Theoharides TC, Enakuaa S, Sismanopoulos N, et al. Contribution of stress to asthma worsening through mast cell activation. Ann Allergy Asthma Immunol. 2012;109(1):14-19. doi:10.1016/j.anai.2012.03.00322727152 Ouvrir le DOISearch in Google Scholar

16. Priftis KN, Papadimitriou A, Nicolaidou P, Chrousos GP. Dysregulation of the stress response in asthmatic children. Allergy. 2009;64(1):18-31. doi:10.1111/j.1398-9995.2008.01948. Ouvrir le DOISearch in Google Scholar

17. Dreger LC, Kozyrskyj AL, HayGlass KT, Becker AB, Mac-Neil BJ. Lower cortisol levels in children with asthma exposed to recurrent maternal distress from birth. J Allergy Clin Immunol. 2010;125(1):116-122. doi:10.1016/j. jaci.2009.09.051 Ouvrir le DOISearch in Google Scholar

18. Haczku A, Panettieri RA Jr. Social stress and asthma: the role of corticosteroid insensitivity. J Allergy Clin Immunol. 2010;125(3):550-558. doi:10.1016/j.jaci.2009.11.005283905920153032 Ouvrir le DOISearch in Google Scholar

19. WHO and United Nations Definition of Adolescent. https://www.publichealth.com.ng/who-and-united-nations-definition-of-adolescent/#:~:text=The%20World%20Health%20Organization%20%28WHO%29%20and%20the%20United,the%20Convention%20on%20the%20Rights%20of%20the%20Child. (accessed on 24th June 2022) Search in Google Scholar

20. Fidalgo S, Ivanov DK, Wood SH. Serotonin: from top to bottom. Biogerontology 2013;14(1):21-45. DOI:10.1007/s10522-012-9406-323100172 Ouvrir le DOISearch in Google Scholar

21. Kim TH, An SH, Cha JY, et al. Association of 5-hydroxytryptamine (serotonin) receptor 4 (5-HTR4) gene polymorphisms with asthma. Respirology (Carlton, Vic.) 2011 May;16(4):630-638. DOI: 10.1111/j.1440-1843.2011.01963.x.21382128 Ouvrir le DOISearch in Google Scholar

22. Shajib MS, Khan WI. The role of serotonin and its receptors in activation of immune responses and inflammation. Acta Physiol (Oxf) 2015;213(3):561-574. DOI:10.1111/apha.12430 Ouvrir le DOISearch in Google Scholar

23. Farjadian S, Moghtaderi M, Fakhraei B, Nasiri M, Farjam M. Association between serotonin transporter gene polymorphisms and childhood asthma. J Asthma 2013;50(10):1031-1035. DOI:10.3109/02770903.2013.834 503 Ouvrir le DOISearch in Google Scholar

24. Heine RG. Preventing atopy and allergic disease. Nestle Nutr Inst Workshop Ser 2014;78:141-153. DOI:10.1159/000354954 Ouvrir le DOISearch in Google Scholar

25. Schatz M, Rosenwasser L. The allergic asthma pheno-type. J Allergy Clin Immunol Pract 2014;2(6):645-649. DOI:10.1016/j.jaip.2014.09.004 Ouvrir le DOISearch in Google Scholar

26. Fuchs O, Bahmer T, Rabe KF, von Mutius E. Asthma transition from childhood into adulthood. Lancet Respir Med 2017;5(3):224-234. DOI:10.1016/S2213-2600(16)30187-4 Ouvrir le DOISearch in Google Scholar

27. Toskala E, Kennedy DW. Asthma risk factors. Int Forum Allergy Rhinol 2015; 5 Suppl 1(Suppl 1): S11-S16. DOI:10.1002/alr.21557715977326335830 Ouvrir le DOISearch in Google Scholar

28. Okada M, Northup JK, Ozaki N, Russell JT, Linnoila M, Goldman D. Modification of human 5-HT(2C) receptor function by Cys23Ser, an abundant, naturally occurring amino-acid substitution. Mol Psychiatry. 2004;9(1):55-64. doi:10.1038/sj.mp.400135714699441 Ouvrir le DOISearch in Google Scholar

29. Land MA, Chapman HL, Davis-Reyes BD, et al. Serotonin 5-HT2C Receptor Cys23Ser Single Nucleotide Polymorphism Associates with Receptor Function and Localization In Vitro. Sci Rep. 2019;9(1):16737. Published 2019 Nov 13. doi:10.1038/s41598-019-53124-2685391631723224 Ouvrir le DOISearch in Google Scholar

30. Human Gene Mutation Database Professional 2022.1, at the Insitute of Medical Genetics in Cardiff. https://my.qiagendigitalinsights.com/bbp/view/hgmd/pro/mut.php?acc=CM981007. (accessed on 25th June 2022) Search in Google Scholar

31. Drago A, Serretti A. Focus on HTR2C: A possible suggestion for genetic studies of complex disorders. Am J Med Genet B Neuropsychiatr Genet 2009;150B(5):601-637. DOI:10.1002/ajmg.b.3086418802918 Ouvrir le DOISearch in Google Scholar

32. Omari-Asor L, Stewart R, Artero S, Ritchie K. 5-HTTLPR genotype, asthma, diabetes and late-life depression in an older French population. Int J Geriatr Psychiatry 2015;30(10):1017-1022. DOI:10.1002/gps.425525640317 Ouvrir le DOISearch in Google Scholar

33. Royo JL. Hardy Weinberg Equilibrium Disturbances in Case-Control Studies Lead to Non-Conclusive Results. Cell J. 2021;22(4):572-574. doi:10.22074/cellj.2021.7195721127332347052 Ouvrir le DOISearch in Google Scholar

34. Choudhury A, Hazelhurst S, Meintjes A, et al. Population-specific common SNPs reflect demographic histories and highlight regions of genomic plasticity with functional relevance. BMC Genomics 2014;15(1):437. Published 2014 Jun 6. DOI:10.1186/1471-2164-15-437.409222524906912 Ouvrir le DOISearch in Google Scholar

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