<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec id="j_bjmg-2022-0006_s_006"><h3>Purpose</h3>
<p>This study aimed at exploring the mechanisms underlying nonalcoholic fatty liver disease (NAFLD) and developing new diagnostic biomarkers for nonalcoholic steatohepatitis (NASH).</p></sec>
<sec id="j_bjmg-2022-0006_s_007"><h3>Methods</h3>
<p>The microarray dataset GES83452 was downloaded from the NCBI-GEO database, and the differentially expressed RNAs (DERs) were screened between the NAFLD and non-NAFLD samples of the baseline and 1-year follow-up time point group based on the Limma package.</p></sec>
<sec id="j_bjmg-2022-0006_s_008"><h3>Results</h3>
<p>A total of 561 DERs (268 downregulated and 293 upregulated) were screened in the baseline time point group, and 1163 DERs (522 downregulated and 641 upregulated) were screened in the 1-year follow-up time point group. A total of 74 lncRNA–miRNA pairs and 523 miRNA–mRNA pairs were obtained in order to construct a lncRNA–miRNA–mRNA regulatory network. Subsequently, functional enrichment analysis revealed 28 GO and 9 KEGG pathways in the ceRNA regulatory network. <italic>LEPR</italic> and <italic>CXCL10</italic> are involved in the Cytokine–cytokine receptor interaction (<italic>P</italic> = 1.86E-02), and the <italic>FOXO1</italic> is involved in both the insulin signaling pathway (<italic>P</italic> = 1.79E-02) and the pathways in cancer (<italic>P</italic> = 2.87E-02).</p></sec>
<sec id="j_bjmg-2022-0006_s_009"><h3>Conclusion</h3>
<p><italic>LEPR</italic>, <italic>CXCL10</italic>, and <italic>FOXO1</italic> were the characteristic target genes for NAFLD.</p></sec>
</abstract>

Purpose
This study aimed at exploring the mechanisms underlying nonalcoholic fatty liver disease (NAFLD) and developing new diagnostic biomarkers for nonalcoholic steatohepatitis (NASH).
Methods
The microarray dataset GES83452 was downloaded from the NCBI-GEO database, and the differentially expressed RNAs (DERs) were screened between the NAFLD and non-NAFLD samples of the baseline and 1-year follow-up time point group based on the Limma package.
Results
A total of 561 DERs (268 downregulated and 293 upregulated) were screened in the baseline time point group, and 1163 DERs (522 downregulated and 641 upregulated) were screened in the 1-year follow-up time point group. A total of 74 lncRNA–miRNA pairs and 523 miRNA–mRNA pairs were obtained in order to construct a lncRNA–miRNA–mRNA regulatory network. Subsequently, functional enrichment analysis revealed 28 GO and 9 KEGG pathways in the ceRNA regulatory network. LEPR and CXCL10 are involved in the Cytokine–cytokine receptor interaction (P = 1.86E-02), and the FOXO1 is involved in both the insulin signaling pathway (P = 1.79E-02) and the pathways in cancer (P = 2.87E-02).
Conclusion
LEPR, CXCL10, and FOXO1 were the characteristic target genes for NAFLD.

Identification of key target genes and pathway analysis in nonalcoholic fatty liver disease via integrated bioinformatics analysis

Balkan Journal of Medical Genetics

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Purpose
This study aimed at exploring the mechanisms underlying nonalcoholic fatty liver disease (NAFLD) and developing new diagnostic biomarkers for...

Identification of key target genes and pathway analysis in nonalcoholic fatty liver disease via integrated bioinformatics analysis

Article Category: Original Article

Publié en ligne: 01 mars 2023

Pages: 25 - 34

DOI: https://doi.org/10.2478/bjmg-2022-0006

© 2022 Chen X., Zhang L., Wang Y., Li R., Yang M., Gao L., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.