<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>The present study was conducted to isolate, identify and characterize a lactic acid bacteria strain from turkey ileum content (46-day-old). The new strain was phenotypical confirmed as <italic>Lactobacillus acidophilus (L. acidophilus)</italic> and conserved under the code IBNA 09. Bacterial profile of <italic>L. acidophilus</italic> was compared with other strains known as <italic>L. paracasei</italic> CCM 1837 and <italic>L. plantarum</italic> ATCC 8014, based on cultural, morphological, biochemical and enzymatic activity (amylase and cellulase). The strains appear as Gram positive bacilli, thin, non-spore-forming, isolated, diplo form, in short chains or in small irregular piles on Man Rogosa and Sharp (MRS) broth and agar medium. The identification and biochemical traits were performed by catalase assay, API 50 CHL V 5.1 soft (L. <italic>acidophilus</italic> biotype 2, 99.9% ID; good identification to the genus <italic>L. paracasei</italic> spp. <italic>paracasei</italic> 1 or 3, 48-51% ID; <italic>L. plantarum</italic> 1, 99.9% ID) and ABIS online <italic>(L. acidophilus</italic> ~ 88%; <italic>L. paracasei</italic> spp. <italic>paracasei,</italic> ~ 90%; <italic>L. plantarum,</italic> ~91%). The highest total score of extracellular amylase activity was recorded by <italic>L. acidophilus</italic> IBNA 09 at 24-48 h (5.10 ± 0.176 U/mL, 4.99 ± 0.409 U/mL), follow by <italic>L. paracasei</italic> CCM 1837(0.12 ± 0.002 U/mL, 0.15 ± 0.001 U/mL). During entire period, cellulase production was observed only for <italic>L. acidophilus</italic> (0.28 ± 0.019 U/mL), comparative with <italic>L. paracasei</italic> where the activity was observed in the first 24 h, respectively at 72 h for <italic>L. plantarum.</italic> These results suggest that <italic>L. acidophilus</italic> IBNA 09 possesses potential probiotic traits as a suitable candidate for amylase and cellulase production, and starter culture can improve cereal fermentation and the process of digestion in poultry nutrition.</p>
</abstract>

The present study was conducted to isolate, identify and characterize a lactic acid bacteria strain from turkey ileum content (46-day-old). The new strain was phenotypical confirmed as Lactobacillus acidophilus (L. acidophilus) and conserved under the code IBNA 09. Bacterial profile of L. acidophilus was compared with other strains known as L. paracasei CCM 1837 and L. plantarum ATCC 8014, based on cultural, morphological, biochemical and enzymatic activity (amylase and cellulase). The strains appear as Gram positive bacilli, thin, non-spore-forming, isolated, diplo form, in short chains or in small irregular piles on Man Rogosa and Sharp (MRS) broth and agar medium. The identification and biochemical traits were performed by catalase assay, API 50 CHL V 5.1 soft (L. acidophilus biotype 2, 99.9% ID; good identification to the genus L. paracasei spp. paracasei 1 or 3, 48-51% ID; L. plantarum 1, 99.9% ID) and ABIS online (L. acidophilus ~ 88%; L. paracasei spp. paracasei, ~ 90%; L. plantarum, ~91%). The highest total score of extracellular amylase activity was recorded by L. acidophilus IBNA 09 at 24-48 h (5.10 ± 0.176 U/mL, 4.99 ± 0.409 U/mL), follow by L. paracasei CCM 1837(0.12 ± 0.002 U/mL, 0.15 ± 0.001 U/mL). During entire period, cellulase production was observed only for L. acidophilus (0.28 ± 0.019 U/mL), comparative with L. paracasei where the activity was observed in the first 24 h, respectively at 72 h for L. plantarum. These results suggest that L. acidophilus IBNA 09 possesses potential probiotic traits as a suitable candidate for amylase and cellulase production, and starter culture can improve cereal fermentation and the process of digestion in poultry nutrition.

Evaluation of <italic>Lactobacillus</italic> spp. Based on Phenotypical Profile as Direct-Fed Microbial Candidate for Poultry Nutrition

Department of Animal Nutrition and Biotechnology, National Research and Development Institute for Biology and Animal Nutrition (IBNA)

Archiva Zootechnica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Evaluation of  spp. Based on Phenotypical Profile as Direct-Fed Microbial Candidate for Poultry Nutrition

The present study was conducted to isolate, identify and characterize a lactic acid bacteria strain from turkey ileum content (46-day-old). The new strain was...

Evaluation of Lactobacillus spp. Based on Phenotypical Profile as Direct-Fed Microbial Candidate for Poultry Nutrition

Publié en ligne: 30 déc. 2021

Pages: 150 - 166

DOI: https://doi.org/10.2478/azibna-2021-0020

Mots clés
identification, enzymatic activity, probiotic, poultry

© 2021 Ioannis Chrysikos, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Evaluation of Lactobacillus spp. Based on Phenotypical Profile as Direct-Fed Microbial Candidate for Poultry Nutrition

Publié en ligne: 30 déc. 2021

Pages: 150 - 166

DOI: https://doi.org/10.2478/azibna-2021-0020

Mots clésidentification, enzymatic activity, probiotic, poultry

© 2021 Ioannis Chrysikos, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Mots clés
identification, enzymatic activity, probiotic, poultry