Molecular mechanisms of colistin resistance mediated by pmrCAB genes in Acinetobacter baumannii isolated from patients hospitalized in Isfahan medical centers Article Category: Original Study Publié en ligne: 28 avr. 2024 Pages: 52 - 57 Reçu: 02 juin 2023 Accepté: 10 janv. 2024 © 2024 Ali Abbasi et al., published by Sciendo This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Figure 1. The results of antimicrobial susceptibility of A. baumannii to the antibiotics investigated in this study. Overall, piperacillin/tazobactam, meropenem, and ciprofloxacin were the most commonly resistant antibiotics (100%). The two most effective antibiotics were colistin (84.5%) and amikacin (9.25%). SAM: Ampicillin-sulbactam; TZP: piperacillin/tozabactam; CAZ: Ceftazidime; FEP: cefepime; MEM: Meropenem; GM: Gentamicin; AN: amikacin; CIP: ciprofloxacin; SXT: Trimethoprim-sulfamethoxazole; and CL: Colistin Figure 2 PCR amplification of the PmrA, B and C genes. Ladder: 100 bp-2kb ladder, lane 1: positive control for PmrA (120bp), B (150bp) and C (132bp) genes; lane 2–9: positive results and negative control Oligonucleotide sequences used in this study
Primer
Primer sequence (5ʹ to 3ʹ)
Amplicon size
Annealing temperature
PmrA (F)
GATGGTTTAAATTTGGGTGCAGAT
120
58
PmrA (R)
TTGACTCGCAAGTTGAGCTTCT
PmrB (F)
GCCATTATTCGTCGTGGTTTAAA
150
57
PmrB (R)
GCGCTCAAAAAGACGGTTCA
PmrC (F)
CCATTTGGCTAGGTGCAATTT
132
57
PmrC (R)
CCGCATAATAGGTAGCAACAAG