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Molecular mechanisms of colistin resistance mediated by pmrCAB genes in Acinetobacter baumannii isolated from patients hospitalized in Isfahan medical centers

Ali Abbasi

Ali Abbasi

Department of Biotechnology, Faculty of Biological Sciences and Technology Shahid Ashrafi Esfahani UniversityIsfahan, Iran
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Abbasi, Ali
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Bahareh Hajihashemi

Bahareh Hajihashemi

Department of Microbiology, Science and Research Branch, Islamic Azad UniversityArak, Iran
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Hajihashemi, Bahareh
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Dariush Shokri

Dariush Shokri

Department of Biotechnology, Faculty of Biological Sciences and Technology Shahid Ashrafi Esfahani UniversityIsfahan, Iran
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Shokri, Dariush
  
28. Apr. 2024
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Artikel-Kategorie: Original Study
Online veröffentlicht: 28. Apr. 2024
Seitenbereich: 52 - 57
Eingereicht: 02. Juni 2023
Akzeptiert: 10. Jan. 2024
DOI: https://doi.org/10.2478/ahem-2024-0002
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© 2024 Ali Abbasi et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1.

The results of antimicrobial susceptibility of A. baumannii to the antibiotics investigated in this study. Overall, piperacillin/tazobactam, meropenem, and ciprofloxacin were the most commonly resistant antibiotics (100%). The two most effective antibiotics were colistin (84.5%) and amikacin (9.25%). SAM: Ampicillin-sulbactam; TZP: piperacillin/tozabactam; CAZ: Ceftazidime; FEP: cefepime; MEM: Meropenem; GM: Gentamicin; AN: amikacin; CIP: ciprofloxacin; SXT: Trimethoprim-sulfamethoxazole; and CL: Colistin
The results of antimicrobial susceptibility of A. baumannii to the antibiotics investigated in this study. Overall, piperacillin/tazobactam, meropenem, and ciprofloxacin were the most commonly resistant antibiotics (100%). The two most effective antibiotics were colistin (84.5%) and amikacin (9.25%). SAM: Ampicillin-sulbactam; TZP: piperacillin/tozabactam; CAZ: Ceftazidime; FEP: cefepime; MEM: Meropenem; GM: Gentamicin; AN: amikacin; CIP: ciprofloxacin; SXT: Trimethoprim-sulfamethoxazole; and CL: Colistin

Figure 2

PCR amplification of the PmrA, B and C genes. Ladder: 100 bp-2kb ladder, lane 1: positive control for PmrA (120bp), B (150bp) and C (132bp) genes; lane 2–9: positive results and negative control
PCR amplification of the PmrA, B and C genes. Ladder: 100 bp-2kb ladder, lane 1: positive control for PmrA (120bp), B (150bp) and C (132bp) genes; lane 2–9: positive results and negative control

Oligonucleotide sequences used in this study

Primer Primer sequence (5ʹ to 3ʹ) Amplicon size Annealing temperature
PmrA(F) GATGGTTTAAATTTGGGTGCAGAT 120 58
PmrA(R) TTGACTCGCAAGTTGAGCTTCT
PmrB(F) GCCATTATTCGTCGTGGTTTAAA 150 57
PmrB(R) GCGCTCAAAAAGACGGTTCA
PmrC(F) CCATTTGGCTAGGTGCAATTT 132 57
PmrC(R) CCGCATAATAGGTAGCAACAAG
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Biologie, Molekularbiologie, Mikrobiologie und Virologie, Medizin, Vorklinische Medizin, Grundlagenmedizin, Immunologie
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