A comparative study of different immunoassays to detect specific antibodies to Echinococcus spp. in human sera
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y | 05 ago 2020
y | 05 ago 2020
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Article Category: Research Article
Publicado en línea: 05 ago 2020
Páginas: 219 - 225
Recibido: 03 feb 2020
Aceptado: 30 mar 2020
DOI: https://doi.org/10.2478/helm-2020-0025
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© 2020 M. Fecková, D. Antolová, K. Reiterová, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Correct seropositive reactions in patients with confirmed alveolar or cystic echinococcosis.
| Antigens/ELISA tests used | |||||
|---|---|---|---|---|---|
| Parasitic disease | |||||
| AgEm | AgB | AgHF | NovaLisaTM | ||
| Alveolar echinococcosis (n = 10) | 9 | n.c. | n.c. | n.c. | 8 |
| Cystic echinococcosis (n = 17) | n.c. | 13 | 17 | 15 | 13 |
False positive reactions with sera of patients suffering from different parasitic/other infections and with control sera.
| Antigens/ELISA tests used | |||||
|---|---|---|---|---|---|
| Parasitic/other infections | |||||
| AgEm | AgB | AgHF | NovaLisaTM | ||
| Alveolar echinococcosis (n = 10) | n.c. | 9 | 7 | 10 | n.c. |
| Cystic echinococcosis (n = 17) | 0 | n.c. | n.c. | n.c. | n.c. |
| Ascariasis (n = 8) | 0 | 0 | 0 | 1 | 0 |
| Trichuriasis (n = 4) | 0 | 0 | 0 | 0 | 0 |
| Trichinellosis (n = 8) | 0 | 0 | 0 | 0 | 1 |
| Toxocariasis (n = 9) | 0 | 0 | 0 | 1 | 0 |
| Toxoplasmosis (n = 8) | 0 | 1 | 1 | 0 | 0 |
| Strongyloidiasis (n = 6) | 0 | 0 | 2 | 1 | 0 |
| Dirofilariasis (n = 1) | 0 | 0 | 0 | 0 | 0 |
| Ricketsiosis (n = 1) | 0 | 0 | 0 | 0 | 0 |
| Clinically healthy persons (n = 25) | 0 | 2 | 1 | 2 | 2 |
Sensitivity, specificity and Youden index of tested antigens/tests.
| Sensitivity (%) | Specificity (%) | PPV (%) | NPV (%) | |||
|---|---|---|---|---|---|---|
| Antigen/test | Parasitic disease | (95% CI) | (95% CI) | (95% CI) | (95% CI) | YI |
| AgEm | 90.0 | 100 | 100 | 98.9 | 0.90 | |
| (55.5–99.8) | (96.6–100) | n.c. | (93.1–99.8) | |||
| 76.5 | 85.0 | 52.0 | 94.4 | |||
| AgB | (50.1–93.2) | (75.4–91.4) | (37.7–66.0) | (87.8–97.6) | 0.61 | |
| 100 | 86.3 | 60.7 | 100 | |||
| AgHF | (83.8–100) | (76.7–92.9) | (47.2–72.8) | n.c. | 0.86 | |
| 88.2 | 83.8 | 53.6 | 97.1 | |||
| (63.6–98.5) | (73.8–91.1) | (40.5–66.2) | (90.1–99.2) | 0.72 | ||
| 77.8 | 95.7 | 87.5 | 91.8 | |||
| NovaLisaTM | (57.7–91.4) | (87.8–99.1) | (69.4–95.6) | (84.6–95.8) | 0.74 |