Accesso libero

A comparative study of different immunoassays to detect specific antibodies to Echinococcus spp. in human sera

INFORMAZIONI SU QUESTO ARTICOLO

Cita

Correct seropositive reactions in patients with confirmed alveolar or cystic echinococcosis.

Antigens/ELISA tests used
Parasitic diseaseE. multilocularisE. granulosus s.l.Echinococcus spp.
AgEmAgBAgHFEchinococcus granulosus(Bordier)NovaLisaTMEchinococcus IgG (NovaTec)
Alveolar echinococcosis (n = 10)9n.c.n.c.n.c.8
Cystic echinococcosis (n = 17)n.c.13171513
Total correctly positive/tested9/1013/1717/1715/1721/27
Sensitivity (%)90.076.510088.277.8

False positive reactions with sera of patients suffering from different parasitic/other infections and with control sera.

Antigens/ELISA tests used
Parasitic/other infectionsE. multilocularisE. granulosus s.l.Echinococcus spp.
AgEmAgBAgHFEchinococcus granulosus (Bordier)NovaLisaTMEchinococcus IgG (NovaTec)
Alveolar echinococcosis (n = 10)n.c.9710n.c.
Cystic echinococcosis (n = 17)0n.c.n.c.n.c.n.c.
Ascariasis (n = 8)00010
Trichuriasis (n = 4)00000
Trichinellosis (n = 8)00001
Toxocariasis (n = 9)00010
Toxoplasmosis (n = 8)01100
Strongyloidiasis (n = 6)00210
Dirofilariasis (n = 1)00000
Ricketsiosis (n = 1)00000
Clinically healthy persons (n = 25)02122
Total false positive/tested0/8712/8011/8013/803/70

Sensitivity, specificity and Youden index of tested antigens/tests.

Sensitivity (%)Specificity (%)PPV (%)NPV (%)
Antigen/testParasitic disease(95% CI)(95% CI)(95% CI)(95% CI)YI
AgEmE. multilocularis90.010010098.90.90
(55.5–99.8)(96.6–100)n.c.(93.1–99.8)
76.585.052.094.4
AgB(50.1–93.2)(75.4–91.4)(37.7–66.0)(87.8–97.6)0.61
10086.360.7100
AgHFE. granulosus(83.8–100)(76.7–92.9)(47.2–72.8)n.c.0.86
88.283.853.697.1
Echinococcus granulosus (Bordier)(63.6–98.5)(73.8–91.1)(40.5–66.2)(90.1–99.2)0.72
77.895.787.591.8
NovaLisaTMEchinococcus IgG (NovaTec)Echinococcus spp.(57.7–91.4)(87.8–99.1)(69.4–95.6)(84.6–95.8)0.74
eISSN:
1336-9083
ISSN:
0440-6605
Lingua:
Inglese
Frequenza di pubblicazione:
4 volte all'anno
Argomenti della rivista:
Life Sciences, Zoology, Ecology, other, Medicine, Clinical Medicine, Microbiology, Virology and Infection Epidemiology