The extent to which lupin seeds can replace soybean meal in diets for broiler chickens is limited, and one of the reasons for this may be a lack of careful consideration given to the age-related amino acid availability differences in the feed formulation process. This study aimed to determine and compare standardised ileal digestibility (SID) coefficients of amino acids (AA) in raw seeds of the Lupinus luteus (YL) and the Lupinus angustifolius (NLL) cultivars as sole sources of AA in the diet of broiler chickens aged 14 or 28 days. An additional purpose was to check the influence of exogenous mono-component protease added to lupin-based assay diets on AA SID in birds at both ages. Each assay diet was offered to six replicatecages. On both sampling days, the contents from the lower half of the ileum were collected for determination of the apparent digestibility values. The SID coefficients were calculated using the age-appropriate basal ileal endogenous AA losses determined from birds fed a N-free diet. Results indicated a substantial advantage of YL over the NLL in SID of Met, Cys, His, Leu, Gly, Asp, Glu, Pro, Tyr and the average of total AA for 14-d-old chickens. With the exception of Cys and Tyr, there was no significant difference between these Lupinus species in the SID of AA in 28-d-old birds. The significant age-related differences within species were found only for the seeds of NLL, where the higher SID for Met, His, Ala, Pro, and the averages of total and indispensable AA were noted in birds aged 28 d. The protease increased SID of most AA from L. luteus in older broilers, whereas for L. angustifolius its efficacy was more pronounced in younger birds, giving the SID values similar to those determined at 28 d on an enzyme-unsupplemented diet. In conclusion, the SID coefficients of indispensable AA determined at 14 or 28 days of age for yellow lupin should not be applied to the precise formulation of starter-type feeds containing seeds of NLL.
