Salivary HPV infection in healthy people
Małgorzata Radwan-Oczko
Department of Oral Pathology, Wrocław Medical UniversityWrocław, Poland
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, Joanna Owczarek-Drabińska
Department of Oral Pathology, Wrocław Medical UniversityWrocław, Poland
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, Anna Szczygielska
Department of Oral Pathology, Wrocław Medical UniversityWrocław, Poland
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, Marta Szczepaniak
Department of Oral Pathology, Wrocław Medical UniversityWrocław, Poland
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y Irena Duś-Ilnicka
Department of Oral Pathology, Wrocław Medical UniversityWrocław, Poland
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29 may 2022
Acerca de este artículo
Categoría del artículo: Original Study
Publicado en línea: 29 may 2022
Páginas: 143 - 148
Recibido: 30 dic 2020
Aceptado: 11 ago 2021
DOI: https://doi.org/10.2478/ahem-2022-0016
Palabras clave
© 2022 Małgorzata Radwan-Oczko et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Total sample description and detection of DNA-HPV in relation with gender and age groups
| Male | 82 | 11 |
| Female | 57 | 3 |
| 19–39 years old | 106 | 12 |
| 40–59 years old | 9 | 0 |
| 60+ years old | 24 | 2 |
Conditioning protocol of nested PCR technique using primer pairs: MY09/11 and GP5+/6+
| MY09/11 | Initial denaturation | 95 | 3 min | - |
| Denaturation | 95 | 30 s | 40× | |
| Hybridization of primers | 53 | 30 s | ||
| Amplification DNA | 72 | 45 s | ||
| Final amplification | 72 | 5 min | - | |
| - | 4 | ∞ | - | |
| GP5+/GP6+ | Initial denaturation | 94 | 5 min | - |
| Denaturation | 94 | 1 min | 30× | |
| Hybridization of primers | 48 | 2 min | ||
| Amplification DNA | 72 | 1 min | ||
| Final amplification | 72 | 5 min | - | |
| - | 4 | ∞ | - |