<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec><h3>Introduction</h3>
<p>In recent years, interest in human papillomavirus infections as a causative factor in epithelial cancer development has grown. Literature indicates that HPV involvement in malignant transformations in oral mucosa can vary significantly, from 0 up to 87%. The aim of our study was to detect the prevalence of salivary HPV infection among generally healthy adults.</p>
</sec>
<sec><h3>Materials and Methods</h3>
<p>The examination involved 139 patients, from whom 139 whole, 1.5ml saliva samples were obtained. HPV DNA was detected by the nested PCR technique. To visualize the PCR products electrophoresis reactions were carried out.</p>
</sec>
<sec><h3>Results</h3>
<p>Sample analysis showed that DNA for HPV was detected in 14 patients: 11 positive results were obtained from men, and 3 from women. This yields a high infection rate: 10.07%.</p>
</sec>
<sec><h3>Conclusions</h3>
<p>The HPV prevalence in the male group was more than twice as high as in the female group. Also, subclinical oral HPV infection was detected more frequently in young (19–39 years old) and older (=>60 years old) adults.</p>
</sec>
</abstract>

Introduction
In recent years, interest in human papillomavirus infections as a causative factor in epithelial cancer development has grown. Literature indicates that HPV involvement in malignant transformations in oral mucosa can vary significantly, from 0 up to 87%. The aim of our study was to detect the prevalence of salivary HPV infection among generally healthy adults.

Materials and Methods
The examination involved 139 patients, from whom 139 whole, 1.5ml saliva samples were obtained. HPV DNA was detected by the nested PCR technique. To visualize the PCR products electrophoresis reactions were carried out.

Results
Sample analysis showed that DNA for HPV was detected in 14 patients: 11 positive results were obtained from men, and 3 from women. This yields a high infection rate: 10.07%.

Conclusions
The HPV prevalence in the male group was more than twice as high as in the female group. Also, subclinical oral HPV infection was detected more frequently in young (19–39 years old) and older (=>60 years old) adults.

Salivary HPV infection in healthy people

Postępy Higieny i Medycyny Doświadczalnej

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

{"article-title":"Salivary HPV infection in healthy people"}

Introduction
In recent years, interest in human papillomavirus infections as a causative factor in epithelial cancer development has grown. Literature...

	Patientsn=139	Positive HPV resultn=14
Male	82	11
Female	57	3
19–39 years old	106	12
40–59 years old	9	0
60+ years old	24	2

Set primers	Reaction step	Temperature, °C	Duration	Number of repetitions
MY09/11	Initial denaturation	95	3 min	-
	Denaturation	95	30 s	40×
	Hybridization of primers	53	30 s
	Amplification DNA	72	45 s
	Final amplification	72	5 min	-
	-	4	∞	-
GP5+/GP6+	Initial denaturation	94	5 min	-
	Denaturation	94	1 min	30×
	Hybridization of primers	48	2 min
	Amplification DNA	72	1 min
	Final amplification	72	5 min	-
	-	4	∞	-

Salivary HPV infection in healthy people

Article Category: Original Study

Publié en ligne: 29 mai 2022

Pages: 143 - 148

Reçu: 30 déc. 2020

Accepté: 11 août 2021

DOI: https://doi.org/10.2478/ahem-2022-0016

Mots clés
human papillomavirus, saliva, healthy adults

© 2022 Małgorzata Radwan-Oczko et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Total sample description and detection of DNA-HPV in relation with gender and age groups

Conditioning protocol of nested PCR technique using primer pairs: MY09/11 and GP5+/6+

Salivary HPV infection in healthy people

Article Category: Original Study

Publié en ligne: 29 mai 2022

Pages: 143 - 148

Reçu: 30 déc. 2020

Accepté: 11 août 2021

DOI: https://doi.org/10.2478/ahem-2022-0016

Mots cléshuman papillomavirus, saliva, healthy adults

© 2022 Małgorzata Radwan-Oczko et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Total sample description and detection of DNA-HPV in relation with gender and age groups

Conditioning protocol of nested PCR technique using primer pairs: MY09/11 and GP5+/6+

Mots clés
human papillomavirus, saliva, healthy adults